摘要
Objective:Todetectthemodulationofcytokinesproductionbyacutepromyelocyticleukemia(APL)cellsbeforeorafterexposuretoall-transretinoicacid(ATRA).Methods:DiagnoseswereperformedaccordingtotheFABcytologicalclassificationcriteriaandcytogeneticcriteria.BonemarroworbloodsamplesfromAPLpatientswerecollectedinheparinizedmicrofugetube.PrimaryAPLcellswereseparatedandpurifiedbytraditionalFicoll-Hypaquedensitycentrifugationandenrichedafteradherencetoplasticsurfaces.IL-1(,IL-6,IL-8,TNF(andG-CSFlevelsinthesupernatantsofculturedleukemiacellswereestimatedbyELISAmethod.NBTmethodwasusedtodetectthedifferentiationofAPLcellsatthesametime.Results:96hafterexposuretoATRAat10-6Minvitroor60mg/dayinvivo,APLcellsshowedasignificantincreaseofIL-1((P<0.05)andG-CSF(P<0.05)production,andasignificantdecreaseofIL-6(P<0.05)andIL-8(P<0.05),however,therewasnoobviousvariationofTNF(.Ontheotherhand,theproliferationofAPLcellsinvitrowasstatisticallycorrelatedtotheIL-1(secretionorG-CSFsecretion.AndthecellnumberratioinpatientswithdetectableIL-1(orG-CSFwashigherthanthatwithoutdetectableIL-1(orG-CSF.Conclusion:IL-1(andG-CSFsecretionmayplayanimportantroleintheproliferationofAPLcellsafterexposuretoATRA.
出版日期
2003年01月11日（中国期刊网平台首次上网日期，不代表论文的发表时间）
