摘要
Soakedindifferentconcentrationsofholmiumnitratesolutionfordifferenttime,roottipsofViciafabawerewashedandfetched.DNAoftheroottipswasextractedandperformedagarosegelelectrophoresis.Slidesoftheroot-tipcellswerepreparedandexaminedundermicroscopeforanomalousnuclei.Meanwhile,root-tipcellswerepreparedandperformedsinglecellgelelectrophoresis.Theresultsshowthatanomalyofnucleiisinducesbyholmiumions.Theresltsalsoshowthatcomettailsarelengthenedandnumbersoftailedcellsareontheincreaseswiththeextensionofdisposalwithin24hatconcentraitionof8mg·L^-1.andthatatdisposalof24h,anobviousandcontinualtapeappearesmagarosegelelectrophoresls,butatdisposalof48h,thecomettailsareshortenedandonlyashorttailingtapeshowsinagarosegelelectrophoresis.ItmaybetheresultofDNArepairorDNAcross-link.Atconcentrationof64mg·L^-1fordisposalof24h,thecomettailsareshorterthanthoseat8mg·L^-1forthesametime.Furthermore,fracturesofnucleiwereobservedundermicroscope,butnotailingtapewasfoundinagarosegelelectrophoresis.NeithertypicalDNAladder,norcharacteristiccomettails'shapeofapoptosiswasfoundintheexperiments.
出版日期
2004年05月15日（中国期刊网平台首次上网日期，不代表论文的发表时间）
