摘要
Soilmicrobialbiomassandcommunitystructuresarecommonlyusedasindicatorsforsoilqualityandfertility.Ainvestigationwasperformedtostudytheeffectsoflong-termnaturalrestoration,cropping,andbarefallowmanagementsonthesoilmicrobialbiomassandbacterialcommunitystructuresindepthsof0–10,20–30,and40–50cminablacksoil(Mollisol).Microbialbiomasswasestimatedfromchloroformfumigation-extraction,andbacterialcommunitystructuresweredeterminedbyanalysisof16SrDNAusingpolymerasechainreaction-denaturinggradientgelelectrophoresis(PCR-DGGE).Experimentalresultsshowedthatmicrobialbiomasssignificantlydeclinedwithsoildepthinthemanagementsofrestorationandcropping,butnotinthebarefallow.DGGEprofilesindicatedthatthebandnumberintop0–10cmsoilswaslessthanthatindepthof20–30or40–50cm.Thesesuggestedthatthemicrobialpopulationwashighbutthebacterialcommunitystructurewassimpleinthetopsoil.ClusterandprinciplecomponentanalysisbasedonDGGEbandingpatternsshowedthatthebacterialcommunitystructurewasa?ectedbysoildepthmoreprimarilythanbymanagements,andthesuccessionofbacterialcommunityasincreaseofsoildepthhasasimilartendencyinthethreemanagements.FourteenpredominatingDGGEbandswereexcisedandsequenced,inwhich6bandswereidentifiedasthetaxaofVerrucomicrobia,2bandsasActinobacteria,2bandsasα-Proteobacteria,andtheother4bandsasδ-Proteobacteria,Acidobacteria,Nitrospira,andunclassifiedbacteria.Inaddition,thesequencesof11DGGEbandswerecloselyrelatedtounculturedbacteria.Thus,thebacterialcommunitystructureinblacksoilwasstable,andthepredominatingbacterialgroupswereuncultured.
出版日期
2009年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
