摘要
Thisstudyinvestigatedthebacterialdiversityofgutcontentofseacucumber(Apostichopusjaponicus)anditshabitatsurfacesedimentinabottomenhancementareausingPCR-baseddenaturinggradientgelelectrophoresis(DGGE)technique.BacterialdiversityevaluationshowedthatthevalueoftheShannon-WienerindexofgutcontentindifferentintestinalsegmentsofA.japonicusvariedbetween2.88and3.00,lowerthanthatofthesurroundingsediment(3.23).PhylogeneticanalysisshowedthatbacterialphylotypesingutcontentandthesurroundingsedimentofA.japonicuswerecloselyrelatedtoProteobacteriaincludingγ-,α-,δ-andε-proteobacteria,Bacteroidetes,Firmicute,andActinobacteria,ofwhichγ-proteobacteriawerepredominant.TheseresultssuggestedthattheseacucumberA.japonicuswascapableoffeedingselectively,andPCR-DGGEwasapplicableforcharacterizingthebacterialcommunitycompositioningutcontentandthesurroundingsedimentofseacucumber.Furtherinvestigationtargetinglonger16SrDNAgenefragmentsand/orfunctionalgeneswasrecommendedforobtainingmoreinformationofthediversityandfunctionofbacterialcommunityinthegutcontentofseacucumber.
出版日期
2014年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
