摘要
TheyoungthalliofPorphyrayezoensisandP.haitanensisareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthatnomutantshavebeeninvestigatedinallcontrolgroups,whileintheinducedgroups,itiseasytofindmutantcellsormutantcellmassesinthethallus.ThepigmentationmutantsmaybetheresultofNGinducement.Themutantrateincreasesobviouslywiththemutagenconcentrationorbyprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofPorphyrayezoensisareraisedfrom11.2%to28.7%,andthoseofP.haitanensisfrom10.1%to20.2%.Accordingtoourexperiments,theamountofmutantcellsinthesameareadoesnotincreasewiththeinductionintensityTheamountofmutantcellsineverygroupofinducementofbothspeciesofPorphyrachangesverylittlewiththefixedfieldscheckmethod.Thelowestdose(25μg/mL)ofthisexperimenthasahighmutantrate.Themutantrateshowsthepercentageofmutantcellsinallthesurvivedcells,whiletheamountofmutantcellsreflectstheeffectofinducement.ThustheoptimuminductionconcentrationofNGis25μg/mL,andtheoptimuminductiontimeis30minutes.
出版日期
2002年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
