摘要
Objective:ToexplorereciprocalactionbetweenBMP-2(bonemorphogeneticprotein-2)andBMP-3forbetterunderstandingofthemechanismofBMPduringbonefractureunion.Methods:rhBMP-2wasaddedintotheculturedfibroblastswiththeconcentrationof1200ng/ml.TheexpressionofBMP-3infibroblastswasdetectedbyimmunohistochemistry.EukaryoticexpressionvectorpcDNA3-BMP-3wastransfectedintothefibroblasts.AftertheeffectiveexpressionofBMP-3wasidentified,BMP-2wasalsodetectedbyimmunohistochemistryinBMP-3expressioncells.ThefibroblaststransfectedwithemptyvectorpcDNA3wereusedasthecontrol.Results:ExogenousrhBMP-2couldpromotetheexpressionofBMP-3infibroblasts.BMP-3alsocouldbedetectedinthesecells.Conclusions:BMP-2andBMP-3couldreciprocallyadjusttheexpressioninfibroblasts.
出版日期
2003年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)