Cloning of Omp1 Gene from Chlamydia trachomatis F Genotype-中国期刊网

摘要 Objective:TodirectionallyclonetheomplgenefromChlamydiatrachomatis(Ct)FGenotypeontoaplasmidvectorforconstructingarudimentaryDNAvaccine.Methods:ThecompleteomplgenefromgenomicDNAofCtFgenotypewildspecieswasamplifiedwithprimersdesignedbycomputer.Therecombinantgenewasobtainedbyrestrictionenzymecutting,linkingthegenewiththeplasmidvectorinvitro,transformingtherecombinantgeneintobacteria,andextractingtheDNAfromthebacteria.Results:DNAextractingfromthebacteriawascomposedoftheimplgeneandplasmid,whichisidentifiedbythreemethodsofsingularrestrictiveenzymecutting,doublerestrictiveenzymecuttingandPCR.Conclusion:CloningoftheomplgenefromtheCtFgenotypemeansthatarudimentaryDNAvaccinewassuccessfullyconstructed.

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Cloning of Omp1 Gene from Chlamydia trachomatis F Genotype

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Cloning of Omp1 Gene from Chlamydia trachomatis F Genotype

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