摘要
Objective:ToestablishafluoregenicprobequantitativeRT-PCR(FQ-RT-PCR)methodfordetectionoftheexpressionofMDR1geneintumorcellsandtoinvestigatetheexpressionofMDR1geneinpatientswithlungcancer.Methods:ThefluorogenicquantitativeRT-PCRmethodfordetectionoftheexpressionofMDR1genewasestablished.K562/ADMandK562celllinesor45tumortissuesfrompatientswithlungcancerwereexaminedonPEAppliedBiosystems7700SequenceDetectionmachine.Results:theaveragelevelsofMDR1geneexpressioninK562/ADMcellsandK562cellswere(6.86±0.65)×107copies/mgRNAand(8.49±0.67)×105copies/mgRNA,respectively.Theformerwas80.8timesgreaterthanthelatter.Eachsamplewasmeasured10timesandthecoefficientvariation(CV)was9.5%and7.9%,respectively.VariouslevelsofMDR1geneexpressionweredetectedin12of45patientswithlungcancer.Conclusion:QuantitativedetectionofMDR1geneexpressionintumorcellswasachievedbyusingFQ-RT-PCR.FQ-RT-PCRisanaccurate,andsensitivemethodandeasytoperform.Usingthismethod,lowlevelsofMDR1geneexpressioncouldbedetectedin24%ofthepatientswithlungcancer.
出版日期
2001年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
