简介:石油开发导致的污染令人堪忧,因此,在石油污染场地对土著石油烃降解菌进行分离鉴定,并进行石油的微生物降解研究是十分必要的。在天津大港油田分离出三株石油烃降解菌株,利用16sRNA基因序列相似性分析确定三株菌株分别为Pseudomonassp.和Bacillussp.。通过单因素实验确定了各菌株最适宜的降解条件为:#1菌株最适宜的温度30°C、pH7.2、盐度3%;#2菌株最适宜的温度35°C、pH7.5、盐度3%;#3菌株最适宜的降解温度37°C、pH7.5、盐度5%。菌株按照2:1:2的比例投加时原油降解率最高。通过正交试验分析,在接菌量10%、原油浓度0.2%、pH7.0、N:P为3:1、盐度3.5%、温度35°C的条件下,原油降解率最高,可达到71.03%。
简介:Fifty-sevenstations(48gridstationsandninestratifiedstations)weresampledacrossthestudyregion(67.000°–88.394°N,152.500°–178.643°W)duringthe4thChineseNationalArcticResearchExpedition(CHINARE4)fromJulytoAugust2010bytheicebreakerR/VXuelong.Atotalof24speciesofHydromedusaewereidentifiedfrom130zooplanktonsamples,ofwhichsevenspeciesbelongedtoAutomedusa,eightspeciestoAnthomedusae,fourspeciestoLeptomedudae,andthreespeciestoSiphonophora.CatablemamulticirratumKishinouye,1910,BougainvilliabitentaculataUchida,1925,andEuphysajaponica(Maas,1909)wererecordedforthefirsttimeintheArcticsea.Inthepresentpaper,18speciesofHydromedusaeweredescribedandillustrated,ofwhichthreespeciesweredescribedforthefirsttimeintheArcticsea,and15speciesweredescribedforthefirsttimeinChina.
简介:类胡萝卜素具有多种生物学活性,红色嗜盐古菌胞膜上大量积累的色素是具有生物技术应用潜力的类胡萝卜素.本文从中国汉沽盐场结晶池卤水中分离得到-株红色单菌落,经16SrRNA基因测序和Blast比对,确定其为极端嗜盐古菌Halobacteriaceae属Haloarcula种.为了确定不同碳源对Haloarcula细胞生长的影响,在含有10g/L酵母提取物和酸水解酪蛋白(4:3,w/w)的培养基中分别添加10g/L蔗糖、乳糖、葡萄糖和可溶性淀粉,摇瓶培养后发现,蔗糖是Haloarcula细胞生长的最适碳源.进一步将5g/L、10g/L和15g/L蔗糖分别加入改良CM培养基,摇瓶培养后发现10g/L蔗糖添加水平最佳.对色素提取物进行TLC和硅胶柱层析分析表明,在Haloarcula胞内有五种主要色素的积累,根据它们的光谱扫描吸收特性初步判定,其中三种色素为番茄红素,菌红素和菌红素的衍生物.
简介:Astochastictwo-dimensionalFornasini-Marchesini'sModelⅡ(2-DFMMⅡ)withmultiplicativenoiseisgiven,andafilteringalgorithmforthismodel,whichisoptimalinthesenseoflinearminimum-variance,isdeveloped.Thestochastic2-DFMMⅡwithmultiplicativenoisecanbereducedtoa1-Dmodel,andtheproposedoptimalfilteringalgorithmforthestochastic2-DFMMⅡwithmultiplicativenoiseisobtainedbyusingthestateestimationtheoryof1-Dsystems.Anexampleisgiventoillustratethevalidityofthisalgorithm.
简介:Inthisstudy,thecombinedactionsofwavesandtidalcurrentsinestuarineandcoastalareasareconsideredanda2Dmathematicalmodelforsedimenttransportbywavesandtidalcurrentshasbeenestablishedinorthogonalcurvilinearcoordinates.Non-equilibriumtransportequationsofsuspendedloadandbedloadareusedinthemodel.Theconceptofbackgroundconcentrationisintroduced,andtheformulaofsedimenttransportcapacityoftidalcurrentsfortheOujiangRiverestuaryisobtained.TheDouGuorenformulaisemployedforthesedimenttransportcapacityofwaves.SedimenttransportcapacityintheformofmudandtheintensityofbacksiltingarecalculatedbyuseofLuoZaosen'sformula.Thecalculatedtidalstagesareingoodagreementwiththefielddata,andthecalculatedvelocitiesandflowdirectionsof46verticallinesfor8crosssectionsarealsoingoodagreementwiththemeasureddata.Onsuchabasis,simulationsofbacksiltingafterexcavationofthewaterwaywithasandbarundercomplicatedboundaryconditionsinthenavigationchannelinducedbysuspendedload,bedloadandmudbywavesandtidalcurrentsarediscussed.
简介:污损生物是影响贝类养殖业生产效率的重要因素。附着在养殖生物贝壳上的污损生物影响养殖生物的生长和存活。大部分污损生物是滤食性捕食者,这些污损生物附着在养殖生物贝壳上竞争食物与空间导致贝类生长速度减慢。本实验主要研究有污损生物附着的扇贝及没有污损附着的扇贝在摄食及排泄上的差异,用以说明污损生物对养殖生物摄食及排泄的影响。本研究对chlorophylla和TPM的吸收速率进行了测定,对NH4-N,NO2-N,NO3-N,PO4-P的排泄速率进行了测定,对摄食的浮游植物进行了种类鉴定。研究结果表明,有污损生物附着的扇贝及没有污损附着的扇贝在对浮游植物及颗粒物的吸收速率上存在明显差异(P〈0.001)。在氨及亚硝态氮排泄速率上也存在明显差异(P〈0.001)。两者吸收NO3-N,且吸收速率无差异。通过对摄食的浮游植物种类的鉴定表明,有污损生物附着的扇贝摄食的浮游植物种类要高于没有污损附着的扇贝,且摄食种类有重叠。对同一类的某些浮游植物,有污损生物附着的扇贝的摄食要高于干净扇贝。本研究结果表明,污损生物对浮游植物的摄食及水中氨氮浓度的提高贡献很大,与养殖生物之间存在食物竞争。
简介:Thegrowth,biochemicalcontentandbioaccumulationquantityof2,2’,4,4’-tetrabromodiphenylether(BDE-47)inSkeletonemacostatumwerestudiedunderdifferentN:Pratios(1,4,16,64and128).AllcellularbiochemicalcontentsofS.costatumpresenteddecreasingtrendovercultivationtime.Atearlystageofcultivation,thecellularprotein,carbohydrateandlipidinS.costatumpresentedhighervaluesintreatmentsofN:P=4and16.However,theywerelowerinthesetreatmentsatthelatestage,buthigherintreatmentsN:P=1and128.Similarly,BDE-47levelspercellofS.costatumwerehigherintreatmentsofN:P=4and16atearlystageofcultivation,whichwere3.8and3.7ng(106cells)-1,respectively.Atthemiddlestageofcultivation,theBDE-47levelperS.costatumcelllowered;anditfurtherreducedinthetreatmentsofN:P=4and16atthelatestagewiththevalues0.6and0.5ng(106cells)-1,respectively.However,itroseinN:P=128,reachingupto2.3ng(106cells)-1.ComparedwithBDE-47percell,BDE-47peralgalvolumeunderdifferentN:Pratiosdidnotpresentobviousdifference.ThequantityBDE-47accumulatedpercellofS.costatumwaspositivelycorrelatedwithprotein,carbohydrateandlipidpercell;meanwhile,theBDE-47pervolumehadapositivecorrelationwithbiochemicalcontentpervolume.ThevariationofbioaccumulationabilityofBDE-47inS.costatumcanbeexplainedbybiochemicalchangesduetoN:Pratios.
简介:Combinedmulti-bodydynamicswithstructuraldynamics,anewdiscreteelementwithflexibleconnector,whichisapplicablefor3-Dbeamstructures,isdevelopedinthispaper.Boththegeneralizedelasticcoefficientmatrixoftheflexibleconnectorandthemassmatrixofdiscreteelementmaybeoff-diag-onalinageneralcase.Thezero-lengthrigidelementisintroducedtosimulatethenodeatwhichmultipleel-ementsarejointedtogether.Itmayalsobeeffectivewhentheaxesofadjacentelementsarenotinthesameline.Theexamplesforeigenvaluecalculationshowthatthemodelissuccessful.Itcanbeextendedtothegeometricnonlinearresponseanalysis.
简介:Acomprehensivestrengthmonitoringsystemusedonafixedjacketplatformispresentedinthispaper.Thelong-termmonitoringofW-11-4Aplatformachieved.Structuralresponses(strainandacceleration)atselectedlocations,aswellasassociatedenvironmentalparameters,havebeenobtained.Theemphasisofthepaperisplacedonthesystemdesign,andtheinstrumentationandoperationmethodologyemployedinthemonitoringofthestructuralresponses.Theperformanceofthesystemandthecharacteristicresultsobtainedduringits13-monthoperationarealsosummarized.
简介:Cytarabine(1-β-D-arabinofuranosylcytosine,Ara-C),isolatedfromaCaribbeanspongespeciesTethyacrypta,isthefirstantitumordrugfromamarineresource.In1980,theUSFoodandDrugAdministrationapprovedthisdrugforthetreatmentofdifferenttypesofleukemia.Thisdrughasashortplasmahalf-life,lowstability,limitedbioavailability,andseveresideeffects.Toimprovestabilityandbioavailability,wesynthesizedninenovelderivativesbyblockingthecytarabinemetabolicsitesandimprovinglipophilicity.ThecLogPvaluesofthenewlysynthesizedcompoundswerecalculated.Allthesynthesizedcompoundsweremorelipophilicthancytarabine,resultinginmembranepermeabilityandbioavailabilityimprovement.TheantitumoractivitiesagainstleukemiacelllineHL-60wereevaluatedbyusingtheMTTassay.ThebioassayresultsrevealedthattheIC50valuesofcompounds5,8and9were0.080,0.090and0.057μmolL-1,respectively,whichwassimilarwiththatofcytarabine(0.056μmolL-1).Incomparison,compound4withaphosphategroupatO5’wasinactive.Becausephosphoesterbondsareeasilyhydrolyzedbyalkalinephosphataseandarecommonlyusedinproducingprodrugstrategies,compound4mightalsobemetabolizedinvivoandgeneratecompound3orevencytarabinethroughamulti-stepreaction.Thus,compound4mightbeapromisingcompoundtobedevelopedasaprodrug.
简介:Inthispaper,thefocusedwavegroupswithdifferentparametersandtheiractionsonaverticalcylinderareexperimentallystudied.Theharmonicwavecharacteristicsofthefocusingwavesareanalyzedbytheadditionandsubtractionofthecrestandtroughfocusingwaves.Theanalyzedresultsshowthathigherorderharmonicscanbegeneratedbecauseoftheinteractionofcomponentwaves.Nonlinearityincreaseswiththeinputtedwaveamplitudeandthefrequencywidthincrement.Further,thewaverun-uparoundtheverticalcircularcylinderisexperimentallystudied.Itincreaseswiththewavesteepnessandtherelativecylinderdiameterincrease.However,thevariationsofwaverun-uparoundthecircularcylinderaredifferent.Theresearchesprovideareferenceforfurthernumericalstudies.
简介:Thispaperpresentsthedetaildesignconditions,designcriteriaandmethodsaswellasdesignconclusionsoftheprocess,structureandcorrosionoftheproductionflowlinesandhighpressurewaterinjectionlinesinBZ34-2/4oilfieldinBohaiBay.AtpresentthisdesignrepresentsthedesigncapabilityandlevelinthefieldofthesubmarinepipelineengineeringofChina.
简介:Atime-domainmethodisappliedtosimulatenonlinearwavediffractionaroundasurfacepiercing3-Darbitrarybody.ThemethodinvolvestheapplicationofTaylorseriesexpansionsandtheuseofperturbationproceduretoestablishthecorrespondingboundaryvalueproblemswithrespecttoatime-independentfluiddomain.AboundaryelementmethodbasedonB-splineexpansionisusedtocalculatethewavefieldateachtimestep,andthefreesurfaceboundaryconditionissatisfiedtothesecondorderofwavesteepnessbyanumericalintegrationintime.Anartificialdampinglayerisadoptedonthefreesurfacefortheremovalofwavereflectionfromtheouterboundary.Asanillustration,themethodisusedtocomputethesecond-orderwaveforcesandrun-uponasurface-piercingcircularcylinder.Thepresentmethodisfoundtobeaccurate,computationallyefficient,andnumericallystable.
简介:Onthebasisofthetemperatureobservationsduring1961-2000inChina,sevencoupledgeneralcirculationmodels'(GCMs)extremetemperatureproductsareevaluatedsuppliedbytheIntergovernmentalPanelonClimateChange's4thAssessmentReport(IPCC-AR4).Theextremetemperatureindicesinusearefrostdays(FD),growingseasonlength(GSL),extremetemperaturerange(ETR),warmnights(TN90),andheatwavedurationindex(HWDI).Resultsindicatethatallthesevenmodelsarecapableofsimulatingspatialandtemporalvariationsintemperaturecharacteristics,andtheirensembleactsmorereliablethananysingleone.Amongthesevenmodels,GFDL-CM2.0andMIROC3.2performancesaremuchbetter.Besides,mostofthemodelsareabletopresentlineartrendsofthesamepositive/negativesignsastheobservationsbutforweakerintensities.Thesimulationeffectsaredifferentonanationwidebasis,with110°Nasthedivision,east(west)ofwhichtheeffectsarebetter(worse)andthepoorerovertheQinghai-TibetanPlateauinChina.Thepredictionsforthe21stcenturyonemissionsscenariosshowthatexceptdecreasesintheFDandETR,otherindicesdisplaysignificantincreasingtrend,especiallyfortheindicesofHWDIandTN90,whichrepresentthenotableextremeclimate.Thisindicatesthatthetemperature-relatedclimateismovingtowardstheextreme.Inthelate21stcentury,theGSLandTN90(HWDI)increasemostnotablyinsouthwestChina(theQinghai-TibetanPlateau),andtheFDdecreasemostremarkablyintheQinghai-TibetanPlateau,northwestandnortheastofChina.ApartfromSouthChina,theyearlychangerangeoftheextremetemperatureisreducedinmostofChina.