简介：

简介：我们曾报道了短梗霉菌产生的高纤维素酶产量98。在这项研究中，羧甲基纤维素酶（CMCase）在培养的细胞。短梗霉98的纯化至均一，与酶的最大产量为4.51U（mg蛋白）-1。SDS-PAGE分析表明，纯化的酶的分子量为67.0kda。具有相当的敏感性为40℃纯化的酶的最适温度，比从其他真菌的cmcases低得多。该酶的最佳pH值为5.6，和活动的个人资料被稳定在一个范围内的酸度（pH5,0-6.0）。这种酶被激活Na＋，Mg2＋，Ca2＋，K＋，Fe2＋和Cu2＋，然而，它是由Fe3＋，Ba2＋，Zn2＋，Mn2＋和银离子抑制。公里和纯化的酶的Vmax值4.7mgml-10.57pmolL-1min-1（mg蛋白）1，分别。只有大小不同的低聚糖，羧甲基纤维素（CMC）释放与纯化的酶水解后。该基因编码的酶是A.霉98个克隆，其中包含一个开放阅读框（eu978473）意义。推导的蛋白质含有酶超家族的保守结构域（糖基水解酶家族5）。的N-末端氨基酸序列的纯化的酶是m-a-p-h-a-e-p-q-s-q-t-t-e-q-t-s-s-g-q-f，这与从克隆的基因推导一致。这表明，纯化的酶是由克隆纤维素酶基因在酵母编码。

简介：以辣椒素为海洋附着生物防污剂,开发了一种用于海水养殖的网衣材料中的防污期长、无毒且高效的防污涂料.辣椒素防污涂料以从天然辣椒中提取的生物活性物质为驱避剂,并不破坏海洋生物链.挂海防污实验证明涂有辣椒素防污涂料的网衣材料具有极佳的防污效果.并进一步讨论了防污涂料的防污效果及影响防污效果的诸多因素.

简介：Amutant(GQQ-M6)ofaSponge-Derivedstreptomycessp.GQQ-10obtainedbyUV-inducedmutationwasusedforproducingprodiginines(PGs).Singlefactorexperimentsandorthogonalarraydesign(OAD)methodswereemployedformediumoptimization.Inthesinglefactormethod,theeffectsofsolublestarch,glucose,soybeanflour,yeastextractandsodiumacetateonPGsproductionwereinvestigatedindividually.InthesubsequentOADexperiments,theconcentrationsofthese5keynutritionalcomponentscombinedwithsalinitywerefurtheradjusted.ThemutantstrainGQQ-M6gavea2.2-foldhigherPGsproductionthanthatoftheparentstrain;OADexperimentsofferedaPGsyieldof61mgL-1,whichwas10timeshigherthanthatoftheinitialGQQ-10strainundertheoriginalcultivationmode.

简介：SixactivecompoundsareisolatedfromthemarinebryozoanBugulaneritina,inhabitingtheNanwanBayintheSouthChinaSea,usingthebioassay-guidedmethodwithacombinationofextractionandpartitionationwithsuitablesolventsaswellasmultiplecolumnchromatographies(SephadexLH-20,ODSandpreparativeHPLC).Theirstructuresareidentifiedasknownbryostatins-bryostatins4,5,6,10,11and18throughintensiveanalysisofthedataofhighresolution2DNMR(600MHz,DQF-COSY,TOCSY,HMQCandROESY)andESI-MS.Amongthem,bryostatins10,11and18areforthefirsttimeobtainedfromthisbryozoanintheSouthChinaSeaandtheyshowsignificantantineoplasticactivitiesinvitro.

简介：基于2011年12月和2012年6月洪、枯季大潮北港上段河道水域的现场水文观测资料,以及1999、2002、2003、2004、2005、2006、2007、2010年历史水文测验资料,分析潮流历时、流速、优势流和含沙量等水沙现状和变化特征,并探讨近年来该水域水沙变化的主要影响因素。结果表明：（1）目前北港上段河道水域落潮占主导优势;青草沙水库和长江大桥建成后,落潮优势明显增强,洪季大潮涨、落潮垂线平均含沙量减少,枯季大潮涨、落潮垂线平均含沙量增多;（2）流域径流量的季节性变化是造成北港上段河道水域垂线平均流速洪季大、枯季小的主要原因;大型工程的建设是该水域落潮优势增强的主要影响因素;北港上段河道水域含沙量变化可能与近岸工程建设、入海泥沙量减少等因素有关。

简介：AWRF-Chemmodelincludingacomprehensivegas-phasenitrogenchemistrymodulewasusedtosimulateaseveredusteventappearingintheeasternChinaon19-25March,2002.ThemodelingresultwellreproducedPM10concentrationsinvariousdistancesfromthedustsourcesandthetransportpathwayoftheduststrom.TheresultsshowedthatboththeconcentrationsandthedrydepositionfluxesofPM10increasedovertheChinaseasduringthedusteventfollowingthepassageofacoldfrontsystem.ThemaximumfluxesofPM10intheYellowSeaandtheEastChinaSeaduringthedusteventwere5.5and8.4timesofthosebeforetheevent,respectively.However,thetemporalvariationsofthedrydepositionfluxesofparticulateinorganicnitrogendifferedovertheYellowSeafromthoseovertheEastChinaSea.NitrateandammoniuminthewholenorthernChinarapidlydecreasedbecauseoftheintrusionofdust-loadedairon19March.ThedustplumearrivedintheYellowSeaon20March,decreasingtheparticulateinorganicnitrogeninmassconcentrationaccordingly.TheminimumdrydepositionfluxesofnitrateandammoniumintheYellowSeawereabout3/5and1/6ofthosebeforethedustarrival,respectively.Incontrast,whenthedustplumecrossedovertheYangtzeDeltaarea,itbecameabundantinnitrateandammoniumandincreasedtheconcentrationsanddrydepositionfluxesofparticulateinorganicnitrogenovertheEastChinaSea,wherethemaximumdrydepositionfluxesofnitrateandammoniumincreasedapproximatelyby4.1and2.6timesofthosepriortothedustarrival.