简介：ＡＮｅｗＭｅｔｈｏｄｆｏｒＳｃｒｅｅｎｉｎｇＩｍｍｕｎｅＤｒｕｇｓｂｙＬｉｎｅａｒＳｗｅｅｐＰｏｌａｒｏｇｒａｐｈｙＡＮｅｗＭｅｔｈｏｄｆｏｒＳｃｒｅｅｎｉｎｇＩｍｍｕｎｅＤｒｕｇｓｂｙＬｉｎｅａｒＳｗｅｅｐＰｏｌａｒｏｇｒａｐｈｙ￥ＸｉａｏＦｅｎｇ...

简介：Thymopentinderivative(septapeptide)waspreparedbymeansofthesolid-phasepeptidesynthesismethodinwhichstepwiseandsegmentcondensationswereinvolved.ThestructuresofthekeyintermediateandtargetmoleculewereconfirmedbyESI-MS,elementaryanalysis,IR,1HNMRand13CNMRspectrometriesinourexperiments.Inthisstudy,theauthorsfoundthatboththymopentinderivetive(septapeptide)andthymopentincouldpromoteTcellproliferationandincreaseofE-rosefomationcells.

简介：ToanalyzetheantitumorpotentialandmechanismofactionofsimultaneousNewcastlediseasevirus(NDV)hemagglutinin-neuraminidase(HN)andhumaninterleukin18(hIL-18)genetransferinC57BL/6micewithH22hepatoma,themousemodelwithH22hepatomawasestablishedinC57BL/6mice,andtheantitumoreffectsofthecombinedapplicationofNDVHNandhIL-18wereevaluatedinvivo.Theresultsshowthatthegrowthofestablishedtumorsinmiceimmunizedwithadenovirus(Ad)-HNinconjunctionwithAd-hIL-18wassignificantlyinhibitedcomparedwiththatinmiceimmunizedwithAd-HN,Ad-hIL-18alone,ortheemptyvector(Ad-mock).Furthermore,theimmunizationofmicewithAd-HNinconjunctionwithAd-hIL-18elicitedstrongnaturalkilleractivityandH22tumor-specificcytotoxicTlymphocyte(CTL)responsesinvivo.Inaddition,TcellsfromthelymphnodesofmiceimmunizedwithAd-hIL-18orAd-HN+Ad-hIL-18secretedhighlevelsoftheTh1cytokineIL-2andinterferon-γ(IFN-γ),indicatingthattheregressionoftumorcellsisrelatedtoaTh1-typedominantimmuneresponse.TheseresultsdemonstratethatvaccinationwithNDVHNtogetherwithhIL-18maybeanovelandpowerfulstrategyforcancerimmunotherapy.