简介：AstudywasmadeonthepolycultureoftheredalgaeGracilariatenuistipitataChangetXiaf.LiuiZhangetXia,f.nov.,theshrimpPenaeuspenicillatusAlcockandthemudcrabScyllaserrata(Forskal).Thephotosynthesisofthealgaeproducestheoxygenonwhichtheshrimpandthecrabbreathe.Thealgaearethereproductiveplaceoftheorganismforthefoodoftheshrimpandthecarb,alsoistheshelteroftheshrimpandthecrab.Theshrimpandthecrabregardthealgaeasthevegetable

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简介：<正>ThebrineshrimpArtemiaisoftenabundantlyfoundinhypersalineenvironments.Thelarvae(‘nauplii’)hatchingfromitsdormantembryos(‘cysts’)areacruciallivefooditemforthelarvalstagesoffishandshrimpinaquaculture

简介：OnthebasisoftheresearchofDObudgetorkineticsinshrimppond,themaininfluenceprocessofDOanditsseasonalvariationsarequantitativelydescribedthroughredividingthebudgetprocessandmodifyingthequantitativemethodoftheprocess.Thepercentagesofoxygendemandofvariousprocessesinthetotaloxygendemandaredifferentinshrimpcultivationseasons.Itisshowedthatthedissolvedoxygendemandofmini-organismsisthemajoraffectedfactorofDOinthisenvironmentandapproximatelyaccountsfor64.1～74.1%ofthetotaloxygendemand.Intheearlyperiodofshrimpculture,thedissolvedoxygendemandofallotrophicbacteriadegradingorganicmattersismuchlowerthanthatofphytoplanktonrespiration.Butinthemidterrnandlaterperiod,itisabout50%ofthetotaloxygendemandbecauseofthehigherwatertemperatureandmoreseriousself-pollution.Thedissolvedoxygendemandofsedimentislowerandjust19.1～28.8%,whilethepercentageofshrimpoxygendemandislower.TheeffectofphytoplanktononDOinshrimpculturingwaterhasdualism.Oneistheoxygenproducingprocessofphotosynthesisandtheotheristheoxygenconsumptionprocessofrespiration.Itisestimatedthatthedissolvedoxygendemandofphytoplanktonrespirationisapproximatelyone-fifthoftheoxygenproducedbyphotosynthesisundernormalilluminationconditions.Thedissolvedoxygendemandofallotrophicbacteriadegradingorganicmattersandthetotaloxygendemandofsedimentincrease4timesand1.7timesrespectivelyfromtheearlyperiodtothemidtermandlaterperiod.Obviously,theDOofculturingwatercanbealsogreatlyimprovedbycontrollingtheselfpollutionoforganicmattersduringshrimpculture.

简介：TwelvespeciesofcrustaceanfromFujiancoastwereexaminedforlectinswithdifferentanimalerythrocytes.Serumextractsfromallof12speciesshowedagglutinatingactivityagainstatleasttwotypesoftheerythrocytesused,whichrevealedtheexistenceoflectinsinthesespecies.Therewerefivespecies(Penaeusjaponicus,Lophosquillacostata,Charybdisferiatus,Portunuspelagicus,Scyllaserrata)whoseserumscouldagglutinatealltheerythrocytestested.Thelowestserumproteinconcentrationrequiredtoproduceerythrocytesagglutinationvariedremarkably,rangingfrom0.7μg/mLto8080μg/mL.ThestrongestactivitywasshownintheagglutinationofrabbiterythrocytebyserumofPenaeusvanaminas.Inhibitionassaysperformedwithsevenmono-andbisaccharidesshowedthatagglutinationofquailerythrocytesbyserumsofthreespecies(Portunuspelagicus,ScyllaserrataandSesarmasp.)werenotinhibitedbyanysugars,whileotherswereinhibitedbyatleastthreetypesofsugars.TheassayoftemperatureinfluenceonagglutinatingactivityshowedthatonlyPenaeusjaponicusretainedactivitywhentheserumwasheatedto90℃,andotherserumslosttheiractivityat40℃,50℃,60℃,70℃,80℃for10minutes,respectively.

简介：TheBeijingSHRIMPCenter(BSC)isoneoftheNationalScienceandTechnologyInfrastructures,jointlyrecognizedbytheMinistryofScienceandTechnologyandtheMinistryofFinanceofChina.ItwasestablishedinDecemberof2001andisincorporatedintotheInstituteofGeology,ChinaGeologicalSurveyinBeijing.ThemajorobjectivesoftheBSCaretoundertakeresearchingeochronologyandcosmochronology,theformationandearlyhistoryofthesolarsystemandtheEarthandtectonicevolutionoforogenicbelts.TheBSCalsodevelopsEarthSciencesrelatedinstrumentsandrelatednewdatingtechniquesandmethods.

简介：Thispaperdealsmainlywithetiology,pathology,sympotomatology,harmfulness,preventivemeasuresandtreatmentoflarvalmycosis(causedbyLagenidiumsp.)andofciliatedisease(causedbyZoothamniumsp.)ofpenaeidshrimplarvae.

简介：Afterthesingle-strainabilitiesoforganic-pollutant-degradingbacteriainbioremediationofsedimentenvironmentofshrimpculturearedetermined,themultistraindegradingeffectofthecompositionsofdifferentstrainsismeasured.Theresultsindicatethatthemulti-strainsgroupshavehigherdegradingabilitythanthesingle-straingroups.Three-straingroupsarebetterthantwo-straingroups,andfourstraingroupsarebetterthanthree-straingroupsandfive-straingroups,thegroupscomposedofstrainsLt7222,Lt7511,Fc6308andGy7018hasthebestdegradingeffect,theCODMnremovalrateis73.2%in66h,andgetsto82.7%in114h,30%higherthanthatofthebestsingle-straingroup;GroupsofLt7222,Lt7511,Lt7451andGy7018arethesecond,whoseCODMnremovalrateis82.1%in114h,Itissuggestedthatmulti-speciesbacteriabeusedasfunctionalbacteriainbiorernediationofmaricultureenvironment.

简介：DNAbarcodingisapowerfulapproachforcharacterizingspeciesoforganisms,especiallythosewithalmostidenticalmorphologicalfeatures,therebyhelpingtotoestablishphylogeneticrelationshipsandrevealevolutionaryhistories.Inthisstudy,wechosea648-bpsegmentofthemitochondrialgene,cytochromecoxidasesubunit1(COI),asastandardbarcoderegiontoestablishphylogeneticrelation-shipsamongbrineshrimp(Artemia)speciesfrommajorhabitatsaroundtheworldandfurtherfocusedonthebiodiversityofArtemiaspeciesinChina,especiallyintheTibetanPlateau.SamplesfromfivemajorsaltlakesoftheTibetanPlateaulocatedataltitudesover4,000mshowedcleardifferencesfromotherArtemiapop-ulationsinChina.Wealsoobservedtwoconsistentaminoacidchanges,153A/Vand183L/F,intheCOIgenebetweenthehighandlowaltitudespeciesinChina.Moreover,indelsintheCOIsequencewereidentifiedincystandadultsamplesuniquetotheCoQenpopulationfromtheTibetanPlateau,demonstratingtheneedforadditionalinvestigationsofthemitochondrialgenomeamongTibetanArtemiapopulations.

简介：现在的学习被承担决定紧张超声是否高能减少虾变应原的过敏性质。减少这些变应原的引起过敏症的性质将对过敏个人有益。虾蛋白质摘录和虾肌肉的样品被高紧张的超声对待，水为不同时间时期在0℃or50℃洗澡。对待，未经治疗的样品然后被SDS页，西方的污点和竞争性抑制ELISA(Ci-ELISA)分析决定虾allergenicity。Theresults表演当摘录在0℃被对待时，那高紧张的超声没在allergenicity上有效果。然而，重要减少在主要的虾变应原的水平被观察，钢笔一1，当样品在50℃被对待时。在allergenicitywithCi-ELISA的决心，在IgE绑定的减小也被观察。

简介：Fortilinisamultifunctionalproteinimplicatedinmanyimportantcellularprocesses.SinceinjectionofPm-fortilinreducesshrimpmortalitycausedbywhitespotsyndromevirus(WSSV),thereispotentialapplicationoffortilininshrimpculture.Inthepresentstudy,inordertoimprovetrans-membranetransportationefficiency,theproteintransductiondomainofthetransactivatoroftranscription(TAT)peptidewasfusedtofortilin.ThePichiapastorisyeastexpressionsystem,whichiswidelyacceptedinanimalfeeds,wasusedforproductionofrecombinantfusionprotein.Greenfluorescenceprotein(GFP)wasselectedasareporterbecauseofitsintrinsicvisiblefluorescence.Thefortilin,TATandGFPfusionproteinwereconstructed.Theirtrans-membranetransportationefficiencyandeffectsonimmuneresponseofshrimpwereanalyzedinvitro.ResultsshowedthatTATpeptideimprovedinvitrouptakeoffortilinintothehemocytesandmidgutofLitopenaeusvannamei.Thephenoloxidase(PO)activityofhemocytesincubatedwithGFP-FortilinorGFP-Fortilin-TATwassignificantlyincreasedcomparedwiththatinthecontrolwithoutexpressedfortilin.ThePOactivityofhemocytesincubatedwith200μgmL-1GFP-Fortilin-TATwassignificantlyhigherthanthatinthegroupwiththesameconcentrationofGFP-Fortilin.HemocytesincubatedwithGFP-Fortilin-TATatallconcentrationsshowedsignificantlyhighernitricoxidesynthase(NOS)activitythanthoseinthecontrolorintheGFP-Fortilintreatment.ThepresentinvitrostudyindicatedthatTATfusionproteinimprovedtheimmuneeffectoffortilin.

简介：Thepresentstudywasconductedtoevaluatethefeasibilityofreplacingfishmeal(FM)withbioactivepeptides(BPs)indietofwhiteshrimp(Litopenaeusvannamei).Thechangesingrowthperformance,bodycomposition,non-specificimmunity,andwaterqualitywereexaminedaftertheshrimpwerefedfourdiets,inwhich0%(control),33.3%,66.7%and100%ofFMwasreplacedbyBPs,respectively.ThegroupsweredesignatedasCon,1/3BPs,2/3BPs,and3/3BPs.Atotalof720shrimpwithaninitialbodyweightof1.46±0.78gwerefedtheexperimentaldietsfor56days.Theresultsrevealedthat:1)theweightgainrate(WGR)in1/3BPs,2/3BPs,and3/3BPswassignificantlyhigherthanthatinCon(P<0.05),whilenosignificantdifferencewasfoundonsurvivalrateandfeedconversionratio(FCR);2)thewhole-bodycrudeprotein(CP)andcrudelipids(CL)weresignificantlydifferentamonggroups,whiletherewasnosignificantdifferencebetweencrudeashandphosphoruscontents;3)thelevelsofacidphosphatase(ACP),lysozyme(LZM),superoxidedismutase(SOD),phenoloxidase(PO)andbactericidalactivityincreasedsignificantlywiththeinclusionofBPs;4)intermsofwaterquality,nosignificantdifferencewasfoundinpHanddissolvedoxygenamongdietsduringthewholeexperimentalperiod.Moreover,eventhoughnitriteandammoniumlevelstendedtoincreasewithtime,therewasnosignificantdifferenceamonggroups.TheresultsindicatedthatBPsisanapplicablealternativeofproteinsource,whichcansubstituteFMinthedietsofL.vannamei;itisabletoeffectivelypromotegrowthperformanceandimproveimmunity.Moreover,BPsinthedietshadnonegativeimpactonwaterquality.

简介：通过从中国虾(Fenneropenaeuschinensis)的随机的染色体序列探索微卫星教材，一些微卫星教材与富有的多态的遗传信息被获得，并且三重的PCR用三份教材(RS1101，RS0683和H081教材)被建立。由调整Mg~(2+)的最后的集中，dNTP和教材，并且用一个触摸镇PCR程序，PCR系统的最佳扩大参数被获得，它能成功地在PCR反应放大三份教材。在使中毒的页胶化，三教材RS1101，RS0683和H081的放大DNA碎片能是容易识别了对方。为三重的PCR系统，PPE(渊源排除的可能性)是0。9679，并且DP(辨别力量)是0。999327。用到出身和他们的父母的测试十个人的三重的PCR，一个个人threemicrosatelliteloci在所有从出身被排除，它可能为象虚假失误那样的一些未知理智被混合进出身。三重的PCR将具有在识别F的出身的大实际价值。chinensis。

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简介：在结晶化以后沿着Yangtzecraton和他们的变形历史的西方边缘澄清变形侵入的关系，石油化学并且踪迹元素geochemical人物，标明日期的锆石虾U-Pb，和化学药品从在Kangding的Neoproterozoic变形侵入的共存的角闪石和斜长石的作文()，四川()省，和Yuanmou()，云南()省，被学习。与以前的出版数据结合了，在Yangtzecraton的西方边缘的Kangding花岗石的plutons和Yuanmou建筑群通常在一样的句号形成了，这被显示。在700妈的变形事件广泛地存在，至少在四川从Kangding区域盖住到Eshan()在中央云南的区域。而且，角闪岩外形可以代表山峰地区性的变形状况。

简介：Henglingguan和Beiyu变形granitoids，在Zhongtiaoshan前寒武纪的建筑群的西北分布式，包括trondhjemites和计算碱的monzogranites，与太古代的ZhaiziTTG片麻岩显示恼人的接触。并且Beiyumetamorphicgranitoids主要由trondhjemites组成，在Hujiayu背斜褶层的核心分布式。分别地，标明日期加权平均值~(207)Pb/~(206)Pb变老的数据表演的新虾锆石U-Pb是为Henglingguan变形计算碱的monzogranites和Beiyumetamorphictrondhjemites的2435.9Maand2477妈并且揭示600妈继承了的～2在magmatic锆石的核心。Whole-rockgeochemical数据显示那所有Henglingguan和Beiyu变形trondhjemitesandcalc碱的monzogranites属于遇见了铝土的中等钾、高钾的calc-alkalineseries。这些岩石被相对高的全部的碱内容描绘(Na_2O+K_2O，在to9.08%上面)，弄空的Nb，Ta，P和Ti，和衰退权利的REE模式与对高LREEs/HREEsfractionation中等(吝啬的比率(La/Yb)_n=25)。在多元素蜘蛛图的Henglingguan和Beiyumetamorphictrondhjemites显示negativeRb，Th和K异例由原始披风使正常化。Sm-Nd同位素的数据表明这些granitoids有起始的ε_(Nd)到+2.4和Nd的(t)=-1.2弄空T_(MD)=的披风模型年龄2622马马。所有thesegeochemical特征显示这些granitoids在一条大陆边缘的弧被形成，并且trondhjemites主要从少年的部分融化发源玄武岩的材料并且然而，Henglingguan变形计算碱的monzogranites源于在一条大陆边缘的弧下面在古老的外壳中材料再循环。花岗石的岩浆在他们的形成期间经历了污染和分离结晶。

简介：北山黑山岭位于塔里木板块北部北山裂谷带内．该带侵入岩较为发育，主要为华力西构造岩浆带产物．通过对区内基性岩体同位素测定，获得岩体锆石SHRIMPU—Pb年龄为（404±3）Ma，（375±3）Ma和（280±2）Ma．认为基性岩与裂谷玄武岩和洋中脊玄武岩具同源性质，证实其形成于拉张构造环境．提出该带具岩浆熔离型铜镍矿成矿可能．

简介：Thenitrogen(N)andphosphorus(P)budgetandtheecologicalefficiencyofapolyculturesystemofseacucumber(Apostichopusjaponicus),jellyfish(Rhopilemaesculenta)andshrimp(Fenneropenaeuschinensis)werestudiedinacofferdam,120.2hainsize.Thenutrientsweresuppliedbyspringtideinflow.Intotal,139600kgNyr-1and9730kgPyr-1inputtothesystem;while118900kgNyr-1and2840kgPyr-1outflowedfromthesystemconcurrently,thustheoutflowwas85.7%(N)and29.2%(P)ofinflow.TheproductionofNandPwas889.5kgyr-1and49.28kgyr-1(seacucumber)and204kgyr-1and18.03kgyr-1(jellyfishandshrimp),respectively.TheutilizationrateofNandPbypolyculturedanimalswas7.8‰and6.9‰,respectively,21.9%and38%higherthanthatofmonoculturedseacucumber.Ourresultsindicatedthatthepolyculturesystemwasanefficientculturesystemofanimalsandaremediationsystemofcoastalenvironmentaswell;itscavenged14.3%and70.8%ofNandP,respectively.Suchanecologicalefficiencymaybeimprovedfurtherbyincreasingeitherthestockingdensityorthesizeofseacucumberorboth.

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简介：one-bead-one-compound(OBOC)组合的肽图书馆是一个强大的工具识别ligand和受体相互作用。这里，我们使用了OBOC图书馆技术识别对主要贝壳变应原tropomyosin的免疫球蛋白E(IgE)epitopes特定的mimotopes。有8-12氨基酸残余的OBOC肽图书馆为tropomyosin的IgEmimotopes与贝壳过敏症从病人与浆液样品被屏蔽。25mimotopes从屏蔽被识别，他们到tropomyosin特定的IgE的有约束力的反应被肽ELISA证实。这些mimotopes能基于顺序相同被划分成七簇，并且由聚类的mimotopes的EpiSearch印射的epitope被执行描绘并且证实mimotopes的有效性。五从六预言的epitopes被发现与tropomyosin的以前识别的epitopes重叠。为了推进，证实mimotopes的模仿潜力，BALB/c老鼠被使免疫，mimotopes结合了到锁眼恋栈者hemocyanin和assayed让他们的能力导致tropomyosin特定的抗体。那收到的mimotope免疫被发现有tropomyosin特定的免疫球蛋白G的提高的水平的BALB/c老鼠，然而并非收到了无关的mimotope的老鼠。这研究用整个sera开创OBOC图书馆的成功的申请屏蔽并且识别多重虾变应原mimotopes并且验证他们在vitro使用的模仿潜力，在vivo，并且在silico方法。