简介：Rats(Rattusnorvegicus)havemanyadvantagesovermiceinscientificstudies,forexample,theyaremorerelevanttohumaninphysiologicalandpharmacologicalresponses.Therefore,ratsarebroadlyusedinexperimentalstudies.Therecentbreakthroughinthegenerationofratembryonicstemcells(rESCs)opensthedoortoapplicationofgenetargetingtocreatemodelsforthestudyofhumandiseases.Inaddition,theinvitrodifferentiationofrESCsintoderivativesofthreegermlineswillserveasapowerfultoolandresourcefortheinvestigationofmammaliandevelopment,cellfunction,tissuerepair,anddrugdiscovery.However,thedistinctcultureconditionandsignalinhibitor-dependedmaintenanceofrESCsstandasaconsiderablechallengeforitsinvitrodifferentiation.Toaddressit,weinvestigatedwhetherrESCsarecapableofformingterminaldifferentiatedcardiomyocytes.Wefoundthattheembryoidbodies(EBs)-basedmethodusedinmouseESC(mESC)differentiationfailedtoworkinthecultivationofrESCs.WethenmodifiedthedifferentiationprotocolandsuccessfullydevelopedaninvitrodifferentiationsystemtodifferentiaterESCsintothreeembryonicgermlayers.Byusingthismethod,therESCsformspontaneousbeatingcardiomyocyteswiththepropertiessimilartothosederivedfromfetalratheartsandmESCs.Thisuniquecellularsystemwillprovideanewapproachtostudytheearlydevelopmentandcardiacfunctionaswellastoperformpharmacologicaltestandcelltherapystudy(Grants:theStateMajorResearchProgramofChina(2009ZX09503-024,2010CB945603)andCAS(XDA01030000).

简介：Itiswellestablishedthatstemcellscandifferentiateintocelltypesoftheorganinwhichthesearetransplanted.However,theprocessisveryslowduetolackofunderstandingofsignalsimportantfortheirsurvivalanddifferentiation,mostoptimalstemcellsandtheirplasticity.Limitationsandadvantagesofvariouscellsubtypeswillbedescribed.Therateofstemcellsmobilizationandtheirsurvivalintheischemicenvironmentaremajorobstaclesinengraftmentanddifferentiationofstemcellsformeaningfulrepairoftheinfarctedmyocardium.Manipulationofstemcellswithischemicpreconditioning,combinedgeneandcelltherapytogetherwithsimultaneousactivationofdiversesignalingpathwaysformassivestemcellmobilization®enerationhassignificantimpactontherepairprocessbystemcells.Theseandotherdifficultiesencounteredinefficientuseofvariousstemcellshaveresultedininventionofinducedpluripotentstemcellswhichcouldrevolutionizethestemcellbasedtherapyandtheirapplicationsforunderstandingofhumandiseaseanddrugscreeninginthenearfuture.ReprogrammingofadultcellsintoiPScellswithouttheuseofviralvectorsisamajorchallengetowardsgettingiPScellswithoutviralintegrationintocells.Tomeetthischallengewehaverepro-grammedskeletalmyoblastsintoiPScellswithhighefficiencyusingepigeneticmodifiers.TransplantationofiPScellsderivedpurecardiacprogenitorsintoinfarctedmyocardiumledtoextensiverepopulationofscarareawithfullydevelopedmyocyteswithouttumorformationandresultinginmarkedimprovementincardiacfunction.Reprogrammingwithpurechemicalmeanswillmaketherapeuticuseofthesecellsmoresafer.Targetingtheinducedpluripotentstemcellstowardscardiacprogenitorsandtheirapplicationtowardstransplantationisamajorstepforwardinenhancingthemyocardialrepaircapacitybythesecells.

简介：backgroundBonemarrowmesenchymalstemcells(BMSCs)canbeisolatedandculturedtomanypassages.However,StemcellsincludingBMSCsquicklyundergosenescenceinculture.Thecellsenescenceandmulti-directionaldifferentiationhavehamperedproducingBMSCsinquantitywiththeirundifferentiatedstate.Inthisstudywereportanaturalcompound,vitaminC(Vc),maintainsBMSCsstemproperty.MethodsHumanBMSCswereisolatedfrombonemarrowandpurifiedby1.073g/mLdensitygradientcentrifugation.50ng/mLVcwereaddedtoBMSCsfordifferenttimepoint.FlowcytometrywasusedtodetectcellsurfacemarkersofBMSCswithorwithoutVctreatment.BMSCsproliferationwasanalyzedbyMTTassay.PCR(polymerasechainreaction)andreal-timePCRwereusedfordetectingc-kit,nanog,andOct-4genesexpressionlevels.DNAmethyltransferase(Dnmt)1andDnmt3blevelswerealsodetectedbyreal-timePCR.ResultsFlowcytometryshowedthatafterVctreatmentfor6h,thesurfacemarkersofBMSCswerealmostunchanged.VcincreasedtheproliferationactivityofBMSCsfrom6hto24h.PCRshowedtheexpressionofc-kit,nanog,andoct-4geneswereobviouslyincreasedinVctreatedgroupthancontrolgroupat12h.Real-timePCRshowedthatthelevelofc-kit,nanog,andoct-4geneswereunregulatedfrom6hto12hcomparedwithcontrolgroup.VcalsoincreasedDnmt3bbutnotDnmt1geneexpression.ConclusionsOurresultsshowedVcactsatleastacceleratesBMSCsproliferationandmaintainsstemcellproperty.Inourstudy,wehighlightedamethodofimprovingthespeedofBMSCsgenerationandprovidedadditionalinsightsintothemechanisticbasisofpreventingBMSCssenescence.

简介：ObjectivesToinvestigatetheanti-apoptoticeffectsofmesenchymalstemcells(MSCs)onhypoxicinjuredcardiacmyocytesinvitro.MethodsMSCswereisolatedfrombonemarrowofSprague-Dawley(SD)rats,andcardiacmyocytesfromneonatalrats.Theratcardiacmyocyteswereco-culturedwithMSCsorMSC-conditionedmediainanoxia(95%N2+5%CO2)for72hours.CellapoptosiswasmeasuredbyHoechst33258staining.TheexpressionofBcl-2andBaxincardiacmyocyteswastestedbyWesternBlot.ResultsTheapoptoticratewas51.6%±2.4%whencardiacmyocyteswereculturedincontinuoushypoxiaandwassignificantlydecreasedwhencardiacmyocyteswerecoculturedwithMSCsorMSC-conditionedmedia(15.1%±5.4%and24.0%±4.2%respectively,P<0.001).ThedecreasedexpressionofBaxinthecardiacmyocyteswasgreatlyrelatedtothedecreasingofapoptosis,buttherewasnodifferenceinBcl-2expressionamongthesegroups.ConclusionsCo-culturedMSCsshowedsignificantanti-apoptoticeffectsoncardiacmyocytesincontinuoushypoxia.ThemechanismmaybetheinteractofcelltocellandparacrineofcytokineswhicheffectedtheexpressionofBaxinthecardiacmyocytes.

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简介：ObjectivesToconstructarecombinantplasmidcarryingenhancedgreenfluorescentprotein(EGFP)andhumanvascularendothelialgrowthfactor(VEGF)121geneanddetectitsexpressioninratmesenchymalstemcells(MSCs).MethodsHumanVEGF121cDNAwasamplifiedwithpolymerasechainreaction(PCR)frompCD/hVEGF121andwasinsertedintotheeukaryoticexpressionvectorpEGFPC1.AfterbeingidentifiedwithPCR,doubleenzymedigestionandDNAsequencing.TherecombinantplasmidpEGFP/hVEGF121wastransferredintoratMSCswithlipofectamine.TheexpressionofEGFP/VEGF121fusionproteinweredetectedwithfluorescencemicroscopeandimmunocytochemicalstainingrespectively.ResultsTherecombinantplasmidwasconfirmedwithPCR,doubleenzymedigestionandDNAsequencing.ThefluorescencemicroscopeandimmunocytochemicalstainingresultsshowedthattheEGFPandVEGF121proteinwereexpressedinMSCs48haftertransfection.ConclusionsTherecombinantplasmidcarryingEGFPandhumanVEGFwassuccessfullyconstructedandexpressedpositivelyinratMSCs.ItoffersapromisetoolforfurtherresearchondifferentiationofMSCsandVEGFgenetherapyforischemialcardiovasculardisease.

简介：BackgroundOurpreviousstudyshowedthe150mg/mLfetalcardiacsupernatant(FCS)couldinducedifferentiationofBMSCsintocardiomyocye-likecellswithoutcardiomyocytetouch,butdifferentiationefficiencyisnothighenough.Inhibitionofglycogensynthasekinase-3enhancedtheproliferationandsurvivesofstemcells.Wetestedif6-bromoindirubin-3-oxime(BIO,glycogensynthasekinase-3inhibitor)enhancestheeffectsofFCSondifferentiationofBMSCsandexplorethegrowthfactorsinFCS.MethodsBMSCswereisolatedfromthefemurandtibiaoffour-week-oldmaleSprague-Dawleyratsandco-culturedwithFCS(150mg/mL)thatwasmadefromfetalheartsfromnineteen-daypregnantWistarrats.BIOwithdifferentconcentration(0,1,10,and100nM)wasintroducedinculturedishes.Transforminggrowthfactorbeta1(TGF-β1),bonemorphogeneticprotein2(BMP-2)andAktincardiacsupernatantandculturemediumwereassayedwithELISAmethods.ResultsAfterco-culturingwithFCS,beatingmyotubeswereobservedin25.9%BMSCsdishesafter1to2weeks’culture.ThelevelsofTGF-β1andBMP-2inFCSconcentrationswerenomorethanthatinyoungandadultcardiacsupernatant.AllBIOgroupssignificantlyenhancedtheeffectsofFCSondifferentiationofBMSCsintothecardiomyocyte-likecells(1nM,83%;10nM,73%;100nM,100%).AktlevelswerehigherinBMSCsculturalmediumwithFCS.ConclusionsFCScouldinducethedifferentiationofBMSCsintothecardiomyocyte-likecells.TGF-β1andBMP-2mightnotplayaroleinthedifferentiationofBMSCsinducedbyFCS.BIOenhancedtheeffectsofFCSonthedifferentiationofBMSCsintocardiomyocyte-likecells,whichmightinvolvetheAktpathway.

简介：健康教育是整体护理的重要内容之一,它贯穿于对病人进行整体护理的始终.健康教育的效果对于病人的治疗和护理直接相关,但由于脑卒中病人的特点,使健康教育存在一定的难度,为此,谈一点我们实施的体会.1.脑卒中病人疾病特点和健康教育难点1.1.疾病特点:由于脑卒中病人多数是老年患者.多伴有偏瘫、长期卧床、生活不能自理、言语困难、老年性痴呆等.因此,病人理解力和记忆力明显下降,由于这一年龄段病人年纪较大、文化程度不高、接受能力差、因此,给健康教育带来一定难度,结果往往达不到预期目标.1.2.家庭原因:由于一些家庭经济原因或子女照顾不够,宣教方面得不到家属的配合,而不能取得预期效果.

简介：摘要儿科护理中为了使影响健康的危险行为被降低或消除，养成良好的行行为习惯和生活方式，应进行健康教育，教育人们树立健康意识。使患儿及其家长的自我保健意识和能力得到提高，做好儿科护理，密切护患关系，因此做好健康教育在儿科护理工作中也是至关重要的。

简介：目的观察健康教育对社区高血压干预的效果。方法以某高校社区高血压为研究对象，进行健康教育。结果高血压患者高血压知晓率、服药依从率及血压控制率均明显提高。结论健康教育可提高社区高血压患者的高血压病知晓率，减低患者的血压水平，应在社区广泛开展。

简介：目的探析高血压护理中健康教育的应用效果。方法选取2016年1月至2018年1月于我院就诊的76例高血压病患,采用抽签法随机将其分为两组,各38例。两组患者均行常规护理,研究组搭配健康教育,对比两组患者治疗依从率及住院期间安全事件发生率。结果研究组病患治疗依从率(进食、就寝、服药、检查)高于对照组,差异显著(P<0.05)。研究组安全事件发生率低于对照组,但无明显差异(P>0.05)。结论健康教育应用于高血压护理中可提高患者治疗依从性,减少安全事件,值得临床推广应用。

简介：摘要目的探讨健康教育路径对剖宫产产妇的影响。方法将100例符合剖宫产指征的孕妇按照入院的先后顺序随机分为试验组和对照组，试验组52例，对照组48例。对照组给予剖宫产术常规护理，试验组应用健康教育路径对剖宫产产妇进行护理，观察两组剖宫产手术产妇对健康教育知识的掌握情况及对护理人员满意度情况。结果试验组剖宫产产妇对护理人员满意度、健康知识掌握情况高于对照组（P<0.05），差异均具有统计学意义。结论健康教育路径应用到剖宫产术后，增强了产妇对健康知识的掌握水平，同时提高了对护理工作的满意度，具有重要的临床实践意义。

简介：摘要目的分析我院神经内科开展健康教育的方法及效果评价。方法选取我院2013年6月至2015年2月神经内科患者150例，随机分为研究组和对照组，对照组应用常规护理法，研究组在常规护理基础上，增加健康教育护理，对两组的护理效果进行比较。结果研究组满意度97.33%，明显高于对照组85.33%，，具有统计学意义（P<0.05）；研究组健康知识掌握水平96.00%，明显高于对照组88.00%，具有统计学意义（P<0.05）。结论将健康教育应用于神经内科护理中，取得了良好效果，不仅提高了护理质量，还提高了患者对护理的满意度以及健康知识掌握水平。

简介：每月约有10,000名美国人接受心脏除颤器（ICD）植入治疗，以帮助建立健康的心脏节律并预防心源性猝死。但美国心脏协会提出，装置心脏除颤器患者，同时应植入与之相适应的心理支持。

简介：冠状动脉及其大的分支主要走行于心外膜下脂肪组织或心外膜的深面,但有时可能被浅层心肌纤维覆盖,这部分冠状动脉被称为壁冠状动脉,该心肌纤维称为冠状动脉心肌桥（myocardialbridge,MB）,部分心肌桥病人可有心绞痛、心肌梗死、严重心律失常甚至猝死[1].随着近年来冠状动脉造影（Coro-naryangiography）的广泛开展,冠状动脉心肌桥的检出病例不断增加,心肌桥的临床意义也越来越受到关注,对心肌桥病人的健康教育在其临床症状的改善、治疗依从性及生活质量的提高上有重要的意义.

简介：［摘要］通过多种途径的教育方法，提高了癌症患者接受健康教育的积极性，增强了战胜疾病的信心，使患者在放疗期间减轻或避免了放疗反应和并发症。提示实施的健康教育收到良好效果。

简介：目的探讨健康教育在小儿心肌炎护理中的作用。方法选取2016年12月-2017年12月本院72例小儿心肌炎患儿进行前瞻性对照研究。按照入院时间分为对照组(常规护理)与研究组(常规护理+健康教育)。持续干预2个月,对比两组家属健康知识知晓率及护理满意度。结果干预后,研究组家属健康知识知晓率明显高于对照组,结果有统计学意义(P<0.05);研究组护理满意度评分高于对照组,结果有统计学意义(P<0.05)。结论小儿心肌炎护理中实施健康教育的效果更为理想,能提升家属对疾病健康知识的掌握程度,改善护理满意度,值得进行深入研究和推广。

简介：目的：分析健康教育在高血压病人中的应用价值。方法随机我院2010年1月至2013年12月间收治的高血压病人240例，按照护理方案分为对照组和观察组，对照组给予常规护理，观察组在对照组基础上给予系统性健康教育，比较两组高血压知识知晓程度、用药情况、健康生活改善情况及降压情况。结果观察组病人高血压知识合格率、用药依从性、健康生活改善情况均明显优于对照组，患者降压有效率明显高于对照组（P〈0.05）。结论高血压病人护理中实施健康教育可有效提升患者对疾病相关知识的知晓程度，提高患者用药依从性，提高降压治疗有效性，临床推广应用价值显著。

简介：目的探讨心理护理和健康教育对冠心病心绞痛患者的应用效果。方法选择2015年8月-2017年8月期间我院收治的冠心病心绞痛患者52例为研究对象，均分为两组，对照组为常规护理，观察组为心理护理和健康教育，对比两组护理情况。结果干预后，两组的焦虑、抑郁评分低于干预前，观察组低于对照组，观察组的健康知识掌握程度、护理质量、满意度评分高于对照组。结论心理护理和健康教育对冠心病心绞痛患者的应用效果显著，症状和生活质量得到改善，值得应用。