简介:Inordertoselectasuitablegenotypeforthestudiesongenetictransformation,thedifferenceamongthreegenotypes(DN04-18,DN04-25andDN04-26)ofPopulus×euramericana(Populusdeltoides×P.nigra)intermsofshootregenerationwasinvestigatedinthispaper.AnefficientinvitromicropropagationmethodwassuccessfullyestablishedforgenotypeDN04-18.Thehighfrequencyshootregeneration(90.0%)fromleafdiscswasobtainedingenotypeDN04-18onthemodifiedMurashigeandSkoog(mMS)mediumw...
简介:在激光,微波和类似的技术的进展导致了包含皮组织的热治疗的最近的开发。这些处理的有效性被管理由联合了影响织物的热、机械、生物、神经的回答:一个有利相互作用相对小的疼痛和没有持续副作用地导致一个过程。当前,尽管每个行为的方面到建立并且理解的某个程度,任何一个都不在学科交差的区域存在到日期。一条高度学科交差的途径为学习皮肤的biothermomechanical行为被要求,包含简历热转移,简历力学和生理学。对这个题目恰当的全面文学评论在这篇论文被介绍,盖住四个题目区域:(a)皮肤结构,(b)皮肤简历热转移和热损坏,(c)皮肤简历力学,和(d)皮肤biothermomechanics。为进一步的研究的主要问题,问题,和话题也被构画出。这评论发现在每这些方面的重要进展在最近的年里被完成了。尽管焦点被放在皮肤织物的biothermomechanical行为之上,在这篇论文考察的基本概念和方法论可能也为学习另外的软纸巾是适用的。
简介:Extractingrare-earthelementsfromcoalcouldsoonbeeconomicalinU.S.ExtractedfromthereportofUNIVERSITYPARK,Pa.TheU.S.couldsoondecreaseitsdependenceonimportingvaluablerare-earthelements,accordingtoateamofPennStateandU.S.DepartmentofEnergyresearcherswhofoundacost-effectiveandenvironmentallyfriendlywaytoextractthesemetalsfromcoalbyproducts.UsingbyproductsofcoalproductionfromtheNorthernAppalachianregionoftheU.S.,theteaminvestigatedwhetherachemicalprocesscalledionexchangecouldextractREEsinasafermannerthanotherextractionmethods.Resultsshowthationexchangeismoreenvironmentallyfriendlyandrequiresless
简介:HighqualitynaturalmeteoriticmagnetsuccessfullyproducedinashorttimeSource:websiteofTohokuUniversityResearchersfromTohokuUniversityinJapanhavesucceededinproducingacompletelyrare-earthfreehigh-qualityFeNimagnet.Theteam,ledbyProfessorAkihiroMakinoasprincipalinvestigatorissupportedbyaMEXT(MinistryofEducation,Culture,Sports,ScienceandTechnology,Japan)projectentitled,"Ultra-low
简介:Anopen-endedcoaxiallinereflectionmethodespeciallysuitableformeas-uringthedielectricpropertiesofbiologicaltissueinvivoisdescribed.Thismethodofferstheadvantageofnotrequiringanyspecialpreparationofthesamplestobemeasuredbutaclosecontactwiththeopenendofacoaxialline.Itis,therefore,veryconvenienttoacquirealargenumberofmeasurementdatainbroadbandrapidly.Themethodmayalsobeusedtomeasurethepropertiesofothersubstances.ThemeasuringsystemconsistsofanetworkanalyzercontrolledbyamicrocomputerandcalibratedbyusingANAproceduretoelimi-hatetheinflunceoferrornetworkintroducedbytheadapter,someconnectors,etc.Inor-dertoreachhigheraccuracy,theiterativemethodisusedtodeterminetheparametersoftheequivalentcircuit.Measurementsofpermeativitiesofsomelivingtissueshavebeenper-formedinthefrequencybandof0.5-2GHz.Comparedwiththeresultsknowninsomepapers,thevalidityofthismethodhasbeenconfirmed.Thedifferenceindielectricproper-tiesbetweenlivinganddeadtissues,andthetissuepermeativites(ε)versusfrequencyanddurationofmeasurementafterdeathhavealsobeenmeasured.
简介:Astrongdelegationof12membersheadedbyProf.Dr.lng.BrunoMueller,theVicePresidentandmem-beroftheBoardofthePowerTransmissionandDistributionGroupofSiemensAGcametoChinaonOct.23,1991torunseminarsonSiemenstechnology,introducingsystematicallythetrendsandstate-of-the-artpracticesinpowertransmissionanddistributionsystems.ThedelegationwasontheinvitationoftheDepartmentofIn-ternationalCooperationofMinistryofEnergyandChinaElectricity
简介:AbstractObjective:Cyclophosphamide (CTX), which is commonly used in clinical chemotherapy, has a damaging effect on ovarian follicles. This study aimed to establish a new method to count the number of follicles in mouse ovaries using benzyl alcohol-benzyl benzoate (BABB)-based tissue-clearing technology and evaluate the follicle-damaging effects of different doses of CTX.Methods:C57BL/6 mice were divided into four groups and administered intraperitoneal injections of 0, 40, 80, or 120 mg/kg CTX. The serum levels of estradiol (E2) and follicle-stimulating hormone (FSH) were detected using an ELISA kit. Mouse ovaries were subjected to BABB clearing and labeled with DAPI, β-actin, and DDX4 to observe the ovarian structure and follicles. A three-dimensional software, Imaris, was used to reconstruct the ovarian structure and automatically count the number of follicles. The effects of different CTX doses on the total follicle number and estrous cycle were determined.Results:As the CTX dose increased, E2 levels in CTX mice declined from 212.3 to 57.7 pg/mL; the FSH levels increased from 3.2 to 29 ng/mL. Mouse ovaries became transparent after BABB treatment. After fixation, microscopy, and Imaris processing, immunofluorescence signals of β-actin and DAPI from all levels in intact ovaries could be obtained and follicle number in half ovaries could be automatically counted using anti-DDX4 antibody labeling. In the NC, CTX40, CTX80, and CTX120 groups, the proportion of mice in the diestrus phase was 26.67%, 51.67%, 73.33%, and 95.00%, respectively, and the total follicle number was 2,603, 1,761, 1,043, and 262, respectively. E2 levels were positively correlated with follicle number and FSH levels were negatively correlated with follicle number, indicating that the damaging effect of CTX on follicle number may be dose dependent.Conclusion:BABB can be used to clear intact ovaries from adult mice, and follicle number in half ovaries can be automatically counted. The damaging effect of CTX on follicles and the endocrine system is dose dependent.