摘要
BACKGROUND:Astrocytesreactsensitivelytocerebralischemia,causingreactiveproliferationandactivation,whichmaycontributetotheireffectinprotectingorinjuringneuronalregeneration.Whetheracupuncture,asatreatmentforcerebralischemia,regulatestheactivatedstateofastrocyteshasbecomeafocusofrecentinvestigations.OBJECTIVE:Toobservetheeffectsofelectroacupuncture(EA)onultrastructurechangesandreactiveproliferationofastrocytesinthemarginalzoneoffocalcerebralischemiainrats.DESIGN,TIMEANDSETTING:Randomized,controlledanimalstudy.ThisstudywasperformedattheExperimentalAnimalCenterofGuangzhouUniversityofTraditionalChineseMedicinebetweenDecember2007andJuly2008.MATERIALS:Atotalof90maleWistarratswererandomlydividedintoshamoperated,modelandEAgroups.Eachgroupwassubdividedinto1hour,1,3,7,and21dayspost-cerebralischemiagroups,withsixanimalsforeachtimepoint.Rabbitanti-ratglialfibrillaryacidicprotein(GFAP)andgoatanti-rabbitIgG/tetramethylrhodamineisothiocyanatewereprovidedbyBeijingBiosynthesisBiotechnology.TheG-6805electricacupunctureapparatuswasprovidedbyShanghaiHuayi.METHODS:Heat-coagulation-inducedocclusionofthemiddlecerebralarterywasperformedtoestablishamodeloffocalcerebralischemia,inthemodelandEAgroups.Middlecerebralarterieswereexposedwithoutocclusioninshamoperatedgroup.EAwasappliedimmediatelyaftersurgeryintheEAgroup,4/20Hz,2.0-3.0V,1-3mA,toBaihui(GV20)andDazhui(GV14),for30minutes.Thetreatmentwasperformedonceaday.Theshamoperatedandmodelgroupsdidnotreceiveacupuncture.MAINOUTCOMEMEASURES:Inthemarginalzoneoffocalcerebralischemiainratsatdifferenttimepointsafterintervention,theultrastructurechangesofastrocyteswereobservedbyusingtransmissionelectronicmicroscopy.GFAPexpressioninastrocyteswasalsomeasuredbylaserconfocalscanningmicroscopy.RESULTS:Cellswellingandrapidproliferationofastrocytes
出版日期
2009年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
