简介:Ourpreviousreportshaveshownthatlamininglycopeptides(LN-GPs),thetotalglycopeptidespreparedfromlaminin(LN),canpreventtheexperimentallungmetastasisandlivermetastasisofmousecancercells.Inordertoexploretheanti-metastaticmechanismofLN-GPs,westudiedtheeffectsofLN-GPsonmetastasisrelatedbehaviorsofcancercellsinvitro.LN-GPsdidnotaffectcellsurvival.However,LN-GPsinhibitedcellattachmentandspreadingofS180cellsonLN-andMatrigelsubstrateindose-dependentandtime-dependentmanners.Moreover,inhibitionofcellattachmentandspreadingonMatrigelsubstratesweremuchgreateronMatrigelsubstratethanonLNsubstrate.InthegresenceofLN-GPs,S180cellsonLNsubstratechangedfromaflattenedpolygonalshapetoaroundone,themigrationofS180cellsonLNsubstratedecreased,andthenumberofahighlyinvasivehumanpulmonarygiantcarcinomaPGcellsinvadingMatrigelfilterinaBoydenchamberwasreduced.LN-GPsthushavemultipleinhibitoryeffectsoncancermetastasisrelatedbehaviors.