简介：摘要：慢性胆囊炎是临床常见病。多由慢性细菌感染、结石、化学刺激或急性胆囊炎迁延所致。临床多表现为反复发作的右上腹胀痛或隐痛，伴口干口苦，恶心呕吐、厌食油腻等症状。这些症状在多食油腻及情志不畅后发作或加重。腹部B超可以确诊。本病常为急性胆囊炎症消退后遗留下来的病理状态，且多发生于胆石症的基础上，其病因主要是胆囊长期或间断地受到细菌、结石、梗阻或代谢方面的刺激而导致明显的慢性炎症功能障碍。笔者2019年2 月～2021年10月采用丘墟透照海治疗慢性胆囊炎患者，具有较好的效果，现将结果报道如下。

简介：Mycoplasmagenitaliumisthemaincausativeagentfornon-gonococcalandnon-chlamydialurethritis.P32istheputativesurface-exposedmembraneproteinofM.genitaliumandithassubstaintialidentityinaminoacidsequencewithadhesinproteinP30fromM.pnewnoniae.SinceM.pneumoniaemutantslackingP30proteinisdefectiveincytadherence,P32proteinhasbeenproposedtobeanessentialadhesinimplicatedintheadherenceofM.genitaliumtohostcells.TheprokaryoticexpressionvectorpET-30(+)/p32wasconstructedinthepresentstudy,andtherecombinantproteinwasexpressedinE.coliandpurifiedunderdenaturingcondition.Asdemonstratedbytheimmunoblottinganalysis,therecombinantproteincouldreactwithrabbitantiseraagainstM.genitalium,andadherenceinhibitionassayswerepetformedwithantiseraagainstthisrecombinantprotein.ItwasdemonstratedthatP32proteinapperaredtobeanadhesionproteinofM.genitalium,thusprovidingtheexperimentalbasisforbetterunderstandingofthepathogenesisofM.genitaliuminfectionandforthedevelopmentoftherelatedvaccinesagainsttheinfection.

简介：【摘要】目的：分析 32磷在治疗皮肤血管瘤方面的临床效果。方法：收集 2016年 6月 -2019年 6月我院收治的 52例先天性皮肤血管瘤患者为研究对象，所有患者均接受放射性核素 32磷敷贴治疗，观察患者临床治疗效果。结果： 52例患者中 14例为单纯性毛细血管瘤，有效率为 100.00%； 12例鲜红斑痣，有效率为 83.33%； 14例海绵状血管瘤有效率为 42.86%； 12例混合型血管瘤，有效率为 33.33%。结论：皮肤血管瘤采取 32磷治疗临床效果显著，无瘢痕遗留，美观度高，值得临床推广应用。

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简介：摘要：目的：探讨甲状腺相关性眼病非手术治疗的患者的护理。方法：选取2018年9月至2020年6月于眼科住院的甲状腺相关性眼病非手术治疗的患者作患者为研究对象，对的临床资料进行分析研究，并回顾该患者的护理措施，包括：饮食护理、戒烟护理、药物不良的护理、心理护理、眼部护理、安全护理等，总结经验，本组32例的患者住院期间均达到了较理想的治疗效果，顺利出院。结论：通过对甲状腺相关性眼病非手术治疗患者全方位的护理措施，患者病情得到了很好的控制，增加了患者的自信心，提高了患者生活的质量，提高了患者对护理工作的满意度，值得在临床上推广。

简介：摘要：目的：探讨甲状腺相关性眼病非手术治疗的患者的护理。方法：选取2018年9月至2020年6月于眼科住院的甲状腺相关性眼病非手术治疗的患者作患者为研究对象，对的临床资料进行分析研究，并回顾该患者的护理措施，包括：饮食护理、戒烟护理、药物不良的护理、心理护理、眼部护理、安全护理等，总结经验，本组32例的患者住院期间均达到了较理想的治疗效果，顺利出院。结论：通过对甲状腺相关性眼病非手术治疗患者全方位的护理措施，患者病情得到了很好的控制，增加了患者的自信心，提高了患者生活的质量，提高了患者对护理工作的满意度，值得在临床上推广。

简介：Toinvestigatetheexistenceofthemajoroutermembranepmtein(MOMP)geneLip132in15dominantChinesestrainsof15semgroupsofLeptospirairaerrogansand2intemationalstrainsof2semgroupsofLeptospirabiflexa,andtocloneandconstructtheexpressionsystemaswellastoidentifytherecombinantpmteins,genomicDNAsfromstrainsofleptospirawerepreparedbymutinephenol-chloroformmethod,andthefragmentsoftheLipL32genewiththewholelengthfromthestrainswereamplifiedwithhighfidelityPCR.Thetargetamplificationproductswere,sequencedafterT-Acloning,andtheexpressionsystemforthegenesweretherebyconstructed,ExpressionoftherecombinantproteinswasidentifiedbyusingSDSPAGEafterinductionwithIPTGatdifferentdosages.WesternblotassayswithrabbitantiserumagainstthewholecellofTR/PatocⅠofLeptospiraandimmunizedserumwithrMOMPswereusedtodeterminetheimmunoreactivityandimmunogenicityoftherecombinantproteins.Microscopicagglutinationtestwasusedtodeterminethecross-agglutinationtitresinrabbitseraimmunizedwithrMOMPs,andthecelladherencemodelofLeptospirawasusedtoexaminetheblockingeffectsofrabbitantiseraagainsttheserMOMPs.ItwasfoundthattheLipL32genecouldbefoundinallthe17strainsofLeptospiramentionedabovewithtwodifferentgenotypes,i.e.LipL32/1andLipL32/2.AmountsofexpressionsofrMOMP1andrMOMP2afterIPTGaccountedfor40%and10%ofthetotalbacterialpmteinsrespectively.BothrMOMP1andrMOMP2couldcombinewiththerab-bitantiserumagainstleptospiralTR/PatocⅠ,andcouldinducetheproductionofagglutinationantibodiestothese17strainsofLeptospirawith1:2to1:64MATtitres.Therabbitanti-rMOMP1andanti-MOMP2antibodiesat1:2to1:16dilutionscouldefficientlyblockadherenceofLeptospira.ItconcludesthatalltheLeptospiratestedinthepresentstudypossessLipL32/1orLipL32/2genes,andtheconstructedexpressionsystemcanexpresstherMOMP1andrMOMP2.

简介：ExposureofnaivemurineCD4^+TlymphocytestosuperantigensuchasstaphylococcalenterotoxinB(SEB)inducesastrongproliferativeresponse.ProlongedexposureorsubsequentrestimulationoftherespondingTcellpopulationwithSEBleadstotheapoptoticeventsofactivation-inducedcelldeath(AICD).ThesignalingmechanismresponsiblefortheAICDisatargetofintensiveinvestigation.However,theprecisedownstreamsignahngpathwaysofSEB-inducedAICDremainsunclear.Ourresultshereshowthatthesequentialactivationofcaspase-1/ICE-hkeandcaspase-3/CPP32-hkecysteineproteasesprobablyplaysaroleinthesignalingtransductionofSEB-inducedAICD,butcaspase-3/CPP32-hkeproteasesactivationdoesnotdependoncaspase-1-likeproteasesactivation.HerbimycinA,aspecificinhibitorofproteintyresinekinases,inhibitcaspase-3/CPP32-1ikecysteineproteasesactivation.However,itdoesnotpreventDNAfragmentationofCD4^+TcellsapoptosisinducedbySEB.TheseresultsindicatethatproteintyrosinekinasespathwayisprobablyinvolvedinthesignalingtransductionofCD4^+TcellsapoptosisinducedbySEBand“crosstalks”withthepathwayofcaspase-3/CPP32-1ikeproteasesactivation.