简介:Recombinantvacciniavirushasmanyadvantagesovermorerestrictedvectorslikeretrovirusandadenovirus.Theprovensafetyofvacciniavirus,whichisrestrictedtolocalandtransitoryinfection,favorsclinicalapplicationofvacciniavirustodelivercytokineslocally.
简介:Directgenetransferintosomatictissueinvivoisadevelopingtechnologywithpotentialapplicationforcancergenetherapy.Retrovirusvector,whichwasaneffectivevehicle,stillhassomedisadvantagesingeneratinghightiterrecombinantvectorsandmanipulatingtomediateinvirogenetransfer.Inthispaper,recombinantvacciniavirusvectorencodinghuman
简介:Oncolysate,adebrisoftumorcells,hasbeenproventobeeffectiveintumoractiveimmunotherapy,itwasreportedthatthevacciniavirus,especiallyrecombinantvacciniavirusesencodinghumanIL-2(rVV-IL-2),enhancedtheimmunogenicityoftransfectedtumorcells.Inthisexperiment,themurinemelanomacellB16-F10oncolysatestransfectedbyrVV-IL-2(IL-2VBO)wereusedasvaccine.TheIL-2VBOorTK-VBOwaspreparedbyincubatingB16-F10cellswithrVV-IL-2orrVV-TKat