简介：Objectives:TodescribeandanalyzeHIV/AIDS/STD-relatedhighriskbehaviorsandaffectingfactorsofmenwhohavesexwithmen(MSM)inmainlandChina~Methods:426self-administeredorinterview-basedquestionnairescollectedfromMSMwereanalyzedwithSPSSorSASSystems.Results:OftheMSM,four-fifthshadengagedinoral-genitalintercourse,abouthalfhadengagedinanalintercourse,andinthepastyearmorethantwo-fifthshadcasualsexualpartner(s).Veryfewmaintainedmonogamoussexualrelationships,andnearlyhalfalsohadintercoursewithwomen.Onefourthhadpreviouslybeendiagnosedwithavenerealdiseases.OneHIV+individualwasfoundamongthe40whoreportedhavingbeentestedforHIV.Onlytwomenconsistentlyusedcondomsamongtheone-thirdwhohadeverusedcondoms,despitealmostuniversalknowledgeofHIVanditstransmission.Conclusions:MSMinmainlandChinaareverysexuallyactive,highlightingthepossibilityofaseriousHIVepidemicthatmaybedirectlyspreadtothefemalepopulation.However,AIDSinterventionstargetingMSMfacesevereobstructionsresultingfromculturalbiasandotherfactors.

简介：Objectives:Toevaluatetheefficacyofnestedpolymerasechainreaction(PCR)withfirstvoidurine(FVU)forthediagnosisofMycoplasmahominisinmalepatients.Methods:MatchedFVUspecimensandurethralswabswerecollectedfrom194malepatientswithNongonococcalUrethritisandtestedbynestedPCRandcellculture.Cellculturewasusedasagoldstandardforevaluatingotherassaytechniques.Results:ForFVUnestedPCRassayandFVUcellculture,ourresultsshowedthatthesensitivitywas100%and93.3%;specificitywas97.0%and98.2%;positivepredictivevalue(PPV)was85.7%and90.3%,negativepredictivevalue(NPV)was100%and98.8%,respectively.Thetotalconsistencybetweenthetwotechniqueswas97.4%.Conclusions:ForthediagnosisofMycoplasmahominisinmen,nestedPCRdetectingFVUisahighlysensitiveandspecificmethod.FirstvoidurinecanreplaceswabcultureorPCRintermsofacceptabilityandfeasibility.