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简介：在米饭的Phytochromes被由三个成员，PHYA，PHYB，和PHYC组成的一个基因家庭编码。通过以photomorphogenesis描绘phytochrome异种和野类型(WT)，单个phytochromes的角色preliminarily在调整米饭de青白化，flowering时间和富饶被探索了。然而，很少信息都没关于是否或怎么被报导phytochromes在米饭影响叶绿素生合成和叶绿体开发。在这研究，我们比较了野类型和phyA的叶绿素内容，在任何一个白人光(WL)下面成年或红的phyB和phyAphyB异种点亮(R)。结果建议phyB察觉R断然调整叶绿素生合成，当phyA的角色能仅仅在phyB缺乏的异种被检测时。涉及叶绿素生合成的基因的表示层次的分析表明phytochromes由调整protochlorophylloxidoreductaseA(PORA)影响了叶绿素生合成表示。在叶绿体开发的phyB的角色也被分析，并且结果建议phyB察觉R由影响叶绿体和grana的数字调整叶绿体开发，以及叶绿体膜系统。

简介：有低glutelin内容的瑞斯作为为肾失败影响的病人的功能的食物合适。在米饭的低glutelin内容基因Lgc1有在二高度类似的glutelin基因GluB4和GluB5之间的3.5-kb删除，它在染色体2的短手臂上定位。在低glutelin内容米饭改进选择效率繁殖，指定为InDel-Lgc1-1和InDel-Lgc1-2的二个分子的标记被开发检测低glutelin内容基因Lgc1。双PCR察觉显示二个标记的联合使用能容易把Lgc1的遗传型与不同米饭变化区分开来。作为一种简单、便宜的技术，因此，分子的标记能广泛地被用来与Lgc1基因识别不同变化并且在低glutelin内容米饭的帮助标记的选择适用。

简介：Riceblack-streakeddwarfvirus(RBSDV)isarecognizedmemberofthegenusFijivirus,familyReoviridae.Itsgenomehastendouble-strandedRNA(dsRNA)segments(S1-S10),inwhichthefifthgenomesegment(S5)containstwoopenreadingframes(ORFs)withapartiallyoverlappingregion.ThesecondORFofRBSDVS5encodesaviralnonstructuralproteinnamedp5bwithunknownfunction.Torevealthefunctionofp5b,itsgenewasligatedintothebaitplasmidpGBKT7andanexpressionlibrarycontainingricecDNAswasconstructedusingplasmidpGADT7foryeasttwo-hybridassay.Thebaitproteinp5bwasdetectedinyeastbywesternblot,andtheresultofanauto-activationtestshowedthatp5bcouldnotautonomouslyactivatetheexpressionofreportergenesinyeast.Thenthebaitproteinp5bwasusedforscreeningthecDNAexpressionlibrariesofrice.Genefragmentsofsomepivotalenzymesinvolvedinphotosynthesis,respirationandotherimportantmetabolicprocesses,wereidentifiedtointeractwithp5binyeast,suggestingthattheseinteractionsmayplayrolesinsymptomdevelopmentininfectedplants.

简介：Themajormatesterilegenesinanewphoto/thermo-sensitivegenicmalesterile(PTGMS)lineB06Sofricewereanalyzedbythemanipulationofmixturedistributiontheory.TheresultsindicatedthatapairofmajormalesterilenucleargeneswithlargeeffectswereresponsibleforcontrollingthemalesterilityofB06S.

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简介：Rice(Oryzasativa)issensitivetosalinity,butthesalttoleranceleveldiffersamongcultivars,whichmightresultfromnaturalvariationsinthegenesthatareresponsibleforsalttolerance.High-affinitypotassiumtransporter(HKTs)hasbeenproventobeinvolvedinsalttoleranceinplants.Therefore,wescreenedfornaturalnucleotidepolymorphisminthecodingsequenceofOsHKT1,whichencodestheHKTproteinineightVietnamesericecultivarsdifferinginsalttolerancelevel.Intotal,sevennucleotidesubstitutionsincodingsequenceofOsHKT1werefound,includingtwonon-synonymousandfivesynonymoussubstitutions.Furtheranalysisrevealedthatthesetwonon-synonymousnucleotidesubstitutions(G50TandT1209A)causedchangesinaminoacids(Gly17ValandAsp403Glu)atsignalpeptideandtheloopofthesixthtransmembranedomain,respectively.Toassessthepotentialeffectofthesesubstitutionsontheproteinfunction,the3DstructureofHKTproteinvariantswasmodelledbyusingPHYRE2webserver.Theresultsshowedthatnodifferencewasobservedwhencomparedthosepredicted3DstructureofHKTproteinvariantswitheachother.Inaddition,thecodonbiasofsynonymoussubstitutionscannotclearlyshowcorrelationwithsalttolerancelevel.Itmightbeinterestingtofurtherinvestigatethefunctionalrolesofdetectednon-synonymoussubstitutionsasitmightcorrelatetosalttoleranceinrice.

简介：ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.

简介：Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...

简介：米饭镉(Cd)敏感变异的cadB-1用Agrobacteriumtumefaciens被获得调停的系统。在cadB-1和野类型(WT)的暴露以后米饭幼苗到为有增加外部Cd集中的cadB-1和WT的10d，在根积累到高水平的Cd，茎和叶子的Cd集中的一个范围，并且在cadB-1的幼苗生长的抑制比在WT更严肃。氢过氧化物累积在cadB-1的叶子和根是更高的。减少的谷胱甘肽(GSH)的比率/oxidized谷胱甘肽(GSSG)，ascorbate(ASC)/dehydroascorbate(DHA)和减少的菸碱腺嘌dinucleotide磷酸盐(NADPH)/oxidized菸碱腺嘌dinucleotide磷酸盐(NADP+)在高Cd层次下面在叶子和根两个都比在WT在cadB-1是更低的。ascorbateperoxidase(APX)的活动，谷胱甘肽peroxidase(GR)，dehydroascorbatereductase(DHAR)和monodehydroascorbatereductase(MDHAR)在Cd的高水平的处理下面在叶子和根两个都比在WT在cadB-1是也更低的。我们的结果建议在Cd应力下面，ASC-GSH周期更严重比在WT在cadB-1被禁止，显示变异的cadB-1不太能清除反应的氧种类并且对Cd敏感。

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简介：一个实验被进行用荧光灯的微分显示器(软式磁碟机)方法在干旱应激和正常条件下面在米饭叶子和根比较信使rna表达式差别。一积极碎片被H.A的联合孤立黄页(contained0.1%H.A。黄)分离和宏数组屏蔽方法。比作ArabidopsisthalianaNADPH氧化还原酶基因，它有96%身份。cDNA是1423bp，并且包含了与345氨基酸残余编码蛋白质的1048bp的一个完全的开的读物框架。而且，基因表示水平在正常条件下面比那在干旱应激下面是更高的。在干旱反应下面的NADPH氧化还原酶基因的可能的角色也被讨论。

简介：到在米饭germplasm91-1A2的米饭胆量小蚊的抵抗被识别并且遗传上分析了。米饭人口的F1s从作为一个男父母与米饭材料Jinggui，TN1，W1263(Gm1)，IET2911(Gm2)，BG404-1(gm3)，OB677(Gm4)，ARC5984(Gm5)和Duokang1(Gm6)交叉的91-1A2被导出。到米饭胆量小蚊的所有父母线和F1，BC1F1和F2人口的抵抗被识别。结果证明91-1A2和所有F1s对中国米饭胆量小蚊遗传因子型IV抵抗。到在BC1F1和F2的易受影响的抵抗植物的分离比率被X2测试与1:3和9:7规则给予，建议到中国米饭胆量小蚊遗传因子型IV的91-1A2的抵抗被是新抵抗基因的二主导的基因控制，对已知的米饭胆量小蚊抵抗基因非突变而产生之遗传因子。

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