简介:为了解烟草内生细菌的多样性,采用改良的NA培养基和稀释平板法从不同生长期的烟草的根、茎、叶中分离得到127株内生细菌。利用细菌16SrDNA特异引物扩增得到16SrDNA的部分片段,长约700bp,分别用内切酶MspⅠ、HaeⅢ、AfaⅠ、HinfⅠ对扩增产物进行限制性酶切。16SrDNAPCR-RFLP分析中,在100%相似性水平处,除了cj23、ty16、cg23、wy6、cj25、wy13、my23、ty7、cj14、cg25、cg2、cj13、my11,my27单独成群外,其余菌株分为15个分类操作单元,其中Otu1、Otu2,Otu27类群最大,分别包括了27、18,26个菌株。对29个代表菌株16SrDNA序列系统发育分析表明,这些菌株主要分布于芽孢杆菌(Bacillus)分支,其余菌株分布于葡萄球菌属(Staphylococcus)、微杆菌属(Microbacterium)、单胞菌属(Sphingomonas)、中华根瘤菌属(Sinorhizobium)、土壤杆菌属(Agrobac-terium)、根瘤菌属(Rhizobium)、纳西杆菌属(Naxibacter)、贪铜菌属(Cupriavidus)、寡养食单胞菌属(Stenotrophomonas)、不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)、泛菌属(Pantoea)、克雷伯菌属(Klebsiella)、Bifissio属系统发育分支。通过Shannon多样性指数对不同生长期分离到的菌株进行多样性分析和比较,结果表明团棵期叶片中分离到的内生细菌多样性最大,随后是旺长期,苗期次之,成熟期最小。
简介:FROMWOMEN'SMOUNTAINPRODUCTIONBRIGADESTOWOMEN'STREEFARMS¥ByXieJingxiangYouxicountyissituatedinamountainous,poorlyforestedregio...
简介:Aseriesofnovel(S,S)-2,8-diazabicyclo[4.3.0]nonanecontainingbenzimidazolemoietywassynthesizedandtheirstructuresweredeterminedby1HNMRandHRMS.ThebiologicalactivityresultsshowedthatthesecompoundspossessedmoderateantifungalactivitytosomeplantpathogenousfungiandmoderateinsecticidalactivityagainstMythimnaseparataWalkerandCulexpipienspallens.Compounds6eand6fexhibitedgoodantibacterialactivityagainstSclerotiniasclerotiorum,PhytophthorainfestansandFusariumgraminearum.Thelethalityratesofcompounds6eand6kagainstMythimnaseparataWalkerwere100%at200mg/Landthelethalityratesofcompounds6hand6kagainstC.pippienspallenswere75%at2mg/L.
简介:WOMEN’SPARTICIPATIONINSOCIALFORESTRYByLuoRonghuaiLuoshuiisavillageinNinglangCountyinYunnanProvince.MostofoftheMosuonationalit...
简介:按照《行政许可法》、《农药管理条例》、《农药管理条例实施办法》规定,因更名、迁址、合并、分立、重组、新增定点等原因,2017年3月,核准下列农药生产企业变更农药登记证持有人。
简介:Inrecentyears,thequantityandqualityofglobalforestresourcesaredecliningcontinually.Contrarily,thebothinChinaareincreasing,whichisdeeplyrelatedtothesustainableforestmanagementandeco-systemmanagementinChina.Withthetrendthatworldcommunityknowsmoreabouttheimportantroleofforest,moreandmorefunctionsofforestarerecognized.TheimpactandfunctionsofChina'sforestrydeservemoreconcern.Basedonsomeexperiencesinthefieldofforestryresearch,theauthors...
简介:Sulfurtransformationin3soilsmaintainedinaclosedincubationsystemanditsavailabilitytoplantswereinvestigatedusingcarrier-free^35S-SO4^2-and^35S-labeledryegrassstraw.Forcarrier-freeNa2^35SO4treatment,78%,70%and64%of^35SappliedwerefoundinCa(H2PO4)2-extractaleSfraction,4%,5%and7%inslowlysolubleinorganicS,11%,15%and18%inC-O-S,5%,7%,and6%inC-bondedS,and5%,7%and6%inunidentifiedorganicS120daysafterincubationinblacksoil,cinnamonsoilandchestnutsoil,respectively.Mostof35Suptakebyplantscamefromextractable35SO4^2-,andlittlefromC-O-^35SandC-bonded^35S,Inthetreatmentwith35S-labeledstraw,51%,46%and36%of35SincorporatedwerefoundinCa(H2PO4)2-extractableSfraction,7%,6%and7%inslowlysolubleinorganicS,13%,15%and18%inC-O-S,8%,8%and6%inC-bondedS,and18%,25%and35%inunidentifiedorganicSattheendofincubationinabove-mentonedthreesoils,respectively.HigheravailabilityofC-O-35S,C-bonded35Sandunidentifiedorganic35Sfrom35S-labeledstrawwasobservedin35S-labeledstrawtreatmentcomparedtocarrier-freeNa2^35SO4treatment.
简介:Forestshavemultiplebenefitsandfunctions,includingmitigationofclimatechange.Theimpactsofforestsontheglobalcarboncycleincludeforestsascarbonsinks,wood-basedproductsascarbonsinks,bio-energy,andproductionanduseofnon-timberproducts.Inthepastdecades,forestcoverofChinahasincreasedfrom8.6%to18.21%bylarge-scaleafforestationandconversionofcroplandintoforests.Forestbiomasscarbon(C)stockincreasedfrom4.3PgC(1PgC=1015gC)intheearly1980sto5...