简介：Outerdensefibersarespecificsubcellularcomponentsofmammalspermflagella,apartfromtheaxonemeanditsassociatedproteins.Asoneoftheouterdensefiberscomponents,ODF3isessentialfortheformationofmammalspermflagella.Inthepresentstudy,weisolatedtheOdf3gene,1033bpinlength,fromZhikongscallopChlamysfarreri,whichwasnamedasCf-Odf3.ItwaspredictedthatCf-Odf3encodesaproteinof240aminoacidswhichcontains5discontinuousPro-Gly-Prorepeats.TheCf-Odf3transcriptsweredetectedinbothtestisandovaryofC.farreriatvariousstagesthroughqRT-PCR.TheCf-ODF3proteinsynthesizedbyprokaryoteswaspurifiedusingNi2+-NTAaffinitychromatographyandusedtoproduceitspolyclonalantibody.TheantibodyproductwaseffectiveandspecificaswasexaminedbyWesternblotting.TheCf-Odf3transcriptandCf-ODF3proteinwerelocatedinintragonadalsomaticcells(ISCs),spermatogoniaandspermatocytesofC.farreritestes,whilenotranscriptionofCf-Odf3andsynthesisofCf-ODF3weredetectedinspermatidsandspermatozoa.ThelocationofOdf3expressionisdifferentfromthatofvertebrateswhereODF3issynthesizedspecificallyinspermatidsandspermflagella.Moreover,theCf-Odf3expressionwasalsodetectedinISCs,oogoniaandoocytesofthescallopovaries.OurfindingrevealedadifferentcharacteristicofOdf3expressionbetweenscallopandvertebrates,whichimpliedthatCf-Odf3playedaroleinthegametogenesisofC.farreri.

简介：摘要本文介绍了一种FC型尾纤专用插拔工具，该工具简单方便，维护方便，可提高光纤配线箱尾纤操作的安全作业水平和操作效率。

简介：本文讨论基于两维纤维分布模型的非织造布激光散射测定纤维取向分布函数的基本算法，给出了影响测量结果的漫散强度的确定方法，实际测定了四种不同加工方法的非织造布纤维取向分布函数，并将数据与显微投影法测得的纤维取向分布实测数据进行了比较。结果表明，本文所述的方法适用于不同加工方法的非织造布纤维取向分布函数的测量。