简介：Thewaxygene(Wx)inrice,whichencodesthegranuleboundstarchsynthaseenzyme,isresponsibleforamylosesynthesis.Glutinous(sticky)ricehaslittleornoamylosethatcanbeusedinvariousapplications,suchasbrewing.Inthisstudy,knockoutoftheWxgenewithCRISPR/Cas9technologywasconductedintwoelitejaponicaricelines,Huaidao5(HD5)andSuken118(SK118),aimingtodevelopelitestickyricevarieties.WeachievedsixhomozygousT0plantswithmorethan200bpdeletionintheWxgene,aswellas36wx-HD5and18wx-SK118homozygoustransgene-freeplantsintheT1generation.Theseedsofallthemutantswerewhiteandopaque,similartothoseofstickyrice,andcontainedonly2.6%–3.2%amylose.Resultsofscanningelectronmicroscopyshowedthatthequalityofricedidnotchange.Inconclusion,wesuccessfullydevelopedtwoelitestickyricevarieties.

简介：IntroductionSomewritingisgoodinsentencestructurebuteitherpoorincoherenceorincohesion.Theproblemofcoherenceisthatthesequenceofinformationisnotalwaysclearandtheproblemofcohesionisthatthesequenceofinformationisacceptable,whiletheconnectionbetweenitisnotsogood.Thequestionishowtoanalyzeandimprovethesedifferentweaknessinwriting.Wehavetwomethods.Onetofocusoncoherence,theothertofocusoncohesion.Inthisessay,themethodusedisbasedontheprinciplethatcoherenceandcohesionshouldworktogether.Iusethisapproachasatooltoeditanddisplaytheanalysisofcoherenceandcohesioninthepassageentitled’Father&Son".ThispassageispresentedasaguidedwritingexerciseinCollegeEnglishCourse,IntensiveReading,Band3,p271.

简介：Wepresentamethodtorepresentmulti-resolutionvectorgraphicssuchasroadnetworksorrailwaynetworksinvirtualenvironment.Thesevectordatacanbeinteractivelyeditedandthelandscapeandbeexploredinrealtimeatanyaltitudefromflightviewtocarview.Wedesignacontext-focusedvectordescriptionoflinearcanarealfeatures,withassociatedcustomizeddefinitionpaintertospecifytheirappearance(colorandmaterial)andtheirdisplaymode(detailedmodeorsimplifiedmode).Therearesomespecialproblemsindrawingvectorgraphicsinvirtualenvironment.Floating-pointround-offerrorappearwhenweusealowviewpointtoobservethescene,anditleadstoscenejittering.Drawing3Dwidelinesturnsintoaproblemon3Dterrain.Wedesignaview-basedself-adaptiveinterpolationalgorithmandanoffsetlinegeneratingalgorithmtosolveit.Ourresultsshowhighperformancewithgoodvisualquality.

简介：GesarisagreatheroicepiccreatedcollectivelybyTibetans.Forthousandsofyears,ithasbeencirculatingfarandwidewithinTibetanareasandhasbeenlovedbyTibetans.GesarrepresentsanhistoricalpinnacleofancientTibetanculture.AsnotonlyacrystallizationofTibetan'sintelligence,butalsoaculturaltreasurehouse,Gesarisofhighacademicandaestheticvalue.

简介：AbstractThe development of applications for the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) system has increased greatly in recent years, especially in the area of gene therapy by efficient in vivo genome editing. Although great success has been achieved in repairing and rewriting genomes through homology-directed repair coupled with Cas9 nuclease cleavage, its in vivo efficiency is insufficient for gene therapy. Base editing is a next-generation genome-editing tool that does not involve double-stranded DNA breaks and uses components of the CRISPR system together with other enzymes to make point mutations directly in cellular DNA or RNA. Base editors, composed of an engineered deaminase and a catalytically impaired CRISPR/Cas9 variant, are powerful tools for targeted base editing in cells and organisms. In non-dividing cells, base editors can directly transform one base or base pair into another, efficiently installing a point mutation. Undesired by-products of editing are seldom generated during this procedure. Herein we review the different base-editing platforms, including their deaminase recruitment strategies and editing outcomes, and the in vivo delivery of base editors. Additionally, we summarize therapeutic applications of base editing in disorders of the inner ear.

简介：Precisebaseeditingishighlydesiredinplantfunctionalgenomicresearchandcropmolecularbreeding.Inthisstudy,weconstructedarice-codonoptimizedadeninebaseeditor(ABE)-nCas9toolthatinducedtargetedA·TtoG·Cpointmutationofakeysinglenucleotidepolymorphismsiteinanimportantagriculturalgene.Combinedwiththemodifiedsingle-guideRNAvariant,ourplantABEtoolcanefficientlyachieveadeninebaseeditinginthericegenome.

简介：

简介：ObjectiveThestudyistoidentifythecarrierrateofcommondeafnessmutationinChinesepregnantwomenviadetectingdeafnessgenemutationswithgenechip.MethodsThepregnantwomeninobstetricclinicwithouthearingimpairmentandhearingdisordersfamilyhistorywereselected.Theinformedconsentwassigned.PeripheralbloodwastakentoextractgenomicDNA.Applicationofgeneticdeafnessgenechipfordetecting9mutationalhotspotofthemostcommon4Chinesedeafnessgenes,namelyGJB2(35delG,176del16bp,235delC,299delAT),GJB3(C538T),SLC26A4(IVS72A>G,A2168G)andmitochondrialDNA12SrRNA(A1555G,C1494T).Furthergenetictestingwereprovidedtothespousesandnewbornsofthescreenedcarriers.ResultsPeripheralbloodof430pregnantwomenweredetected,detectionofdeafnessgenemutationcarriersin24cases(4.2%),including13casesoftheGJB2heterozygousmutation,3casesofSLC26A4heterozygousmutation,1casesofGJB3heterozygousmutation,and1caseofmitochondrial12SrRNAmutation.18spousesand17newbornstookfurthergenetictests,and6newbornsinheritedthemutationfromtheirmother.ConclusionThecommondeafnessgenesmutationhasahighcarrierrateinpregnantwomengroup,235delCandIVS7-2A>Gheterozygousmutationsarecommon.

简介：Genestellcellswhattodo,forexample,whentorepairDNAmistakesorwhentodie,andcanbeturnedonorofflikealightswitch.Knowingwhichgenesareswitchedon,orexpressed,isimportantforthetreatmentandmonitoringofdisease.Now,forthefirsttime,Researchersinthelaboratoryof

简介：Wehavereviewedthegenetherapyingastrointestinaldiseases^[1].GastriccanceriscommoninChina^(2-20),anditsearlydiagnosisandtreatmentarestilldifficultuptonow^(13-36).Theex-pressionofanexogenousgeneintroducedbygenetherapyintopa-tientswithgliomascanbemonitorednon-invasivelybypositron-emissiontornography^[4].

简介：Objective:TostudythechangesofthegeneexpressionpatternofspinalcordtissuesintheearlystageafterinjurybyDNAmicroarray(genechip).Methods:ThecontusionmodelofratspinalcordwasestablishedaccordingtoAllen'sfallingstrikemethodandthegeneexpressionpatternsofnormalandinjuredspinalcordtissueswerestudiedbygenechip.Results:Theexpressionof45geneswassignificantlychangedintheearlystageafterspinalcordinjury,inwhich22genesup-regulatedand23genesdown-regulated.Conclusions:Theexpressionofsomegeneschangessignificantlyintheearlystageafterspinalcordinjury,whichindicatesthecomplexityofsecondaryspinalcordinjury.

简介：这篇论文为基于的自我类似论述一个便宜方法由使用高度和反照率地图真实世界的3D表面质地编辑。编辑的基于的2Dtexture接近仅仅做变化到象素颜色或紧张的不同自我类似珍视，这种技术也允许表面几何学和捕获的3D表面质地的反射到用原版不同于那些的照明条件和视场角是ited和relit。在一个给定的地点的单个编辑操作影响所有类似的区域并且在在不同条件下面显示的样品的所有图象上生产变化。后来，表面高度和反照率地图能被用来描述海床地形学和地质的特征，它在许多海洋的进程起重要作用，建议方法能有效地关于海洋的现象的可视化和模拟在应用程序被采用。

简介：客观；在忍受肝癌症的老鼠调查鼠科的IL-12基因和HSV-TK基因治疗的synergistic反肿瘤效果。方法：老鼠肝癌症MM45T李(H-2d)房间是有包含IL-12基因或HSV-TK基因的retroviral向量的transfected插入修改基因的肝癌症房间，MM45TLi/IL-12和MM45TLi/TK，与IL-12和TK的稳定的表示被获得。Balb/c老鼠与2X105MM45T李房间皮下地被接种。当肿瘤到达了0.5鈥?.0厘米的一种尺寸时，MM45T.Li/TK房间的混合物照耀and60Co的MM45TLi/IL-12房间intratumoraly被注射。Ganciclovir(GCV)是注射ip(40mg.kg?1.d?1)为10天。Intratumoral注射照耀of60Co的MM45TLi/IL-12房间分开在一个星期内被重复两次。有远肿瘤的老鼠根据一样的协议被对待。怒气房间的CTL活动是测量by51Cr版本试金和由染色的immunohistochemical渗入淋巴细胞的肿瘤的显型。结果：在与MM45T对待的老鼠，Li/IL-12或MM45TLi/TK+GCV个别地在肿瘤生长，而是两个都不导致了中等减小能根除肿瘤完全，当时在加GCV与MM45TLi/IL-12和MM45TLi/TK细胞的混合物对待的60%老鼠，完全的肿瘤回归被观察，没有为二个月的肿瘤复发。远肿瘤的生长也在同样对待的老鼠显著地被禁止。大多数收到的老鼠加GCV联合了基因治疗有的丰富的CD4+，CD8+T淋巴细胞渗入。他们的CTL活动比在老鼠显著地高收到的单个基因治疗。有加GCV的IL-12基因和HSV-TK基因的结论联合治疗为老鼠肝癌症是有效的。

简介：MicroRNAs(miRNAs)是他们在植物和动物的目标基因的重要post-transcriptional管理者。miRNAs通常长是20-24核苷酸。尽管有他们的不平常地小的尺寸，miRNA基因家庭的进化历史似乎类似于编码theirprotein对应物。与在动物染色体的小却丰富的miRNA家庭相对照，植物有少数但是更大的miRNA基因家庭。植物miRNA基因家庭的成员经常是高度类似的，建议经由双人脚踏车基因复制和部分复制事件的最近的扩大。尽管许多miRNA基因越过植物种类被保存，一样的基因家庭在不同种类在尺寸和genomic组织显著地变化，它可以在目标基因规定引起剂量效果和空间、时间的差别。在这评论，我们在理解植物miRNA基因家庭的进化总结当前的进步。

简介：ＥＬＥＭＥＮＴＡＬＳＴＵＤＹＯＦＧＥＮＥＴＨＥＲＡＰＹＷＩＴＨＴＨＲＯＭＢＯＰＯＩＥＴＩＮＬｕＣｈｅｎｇｒｏｎｇ陆承荣ＺｈａｏＪｉａｎｚｅｎｇ赵建增ＷａｎｇＸｉａｏｐｉｎｇ王小平Ｌｉｕｌｉ刘丽ＲｅｓｅａｒｃｈＣｅｎｔｅｒｏｆＭｏｌｅｃｕｌａｒＢｉｏｌ...

简介：Duetoconcernsregardingtheoverlappingfiguresinthisreviewthatareidenticaltothosecontainedinareviewarticlethatwehaveco-authoredandpublishedearlier,weretracttheabovepaperwepublishedinCellResearch.Weapologizeforanyconfusionthatmaybecausedbythismatter,althoughwestandbythescientificcontentscontainedintheCellResearchpaper.

简介：Asearlyas2000yearsago,ancientChinesemedicalrecordshaddescribedtherelationshipbetweendiseasesandappearanceindetail.Moreover,modernmedicinehasalsoconstantlystudiedtherelationshipbetweenfacialfeaturesandhealthinevolutionaryterms.Itiswellknownthatmanyhereditarydiseasesinvolvecertainabnormalfacialfeaturesandgenemutations.Thetumorisalsoconsideredasgeneticdisordertosomeextent,sowhatistherelationshipbetweencancergeneticsandcongenitaldevelopmentoffacialfeatures?Here,wereviewedsomecluestotheappearance-gene-tumorrelation,whichmightbecomethetargetsintheearlypreventionorgenetherapyofcancerinthefuture.Thissummaryprovidedusanewstrategyforthecancergeneticscreeningandanewresearchdirectionforgeneticdiagnosisofthepotentialdisease.

简介：Directgenetransferintosomatictissueinvivoisadevelopingtechnologywithpotentialapplicationforcancergenetherapy.Retrovirusvector,whichwasaneffectivevehicle,stillhassomedisadvantagesingeneratinghightiterrecombinantvectorsandmanipulatingtomediateinvirogenetransfer.Inthispaper,recombinantvacciniavirusvectorencodinghuman

简介：Thehomeobox(Hox)genesformanevolutionarilyconservedfamilyencodingtranscriptionfactorsthatplaymajorrolesinsegmentalidentityandorganspecificationacrossspecies.ThecanonicalgroupingofHoxgenespresentintheHOM-CclusterofDrosophilaorrelatedclustersinotherorganismsincludeseight"typical"genes,whicharelocalizedintheorderlabial(lab),proboscipedia(pb),Deformed(Dfd),Sexcombsreduced(Scr),Antennapedia(Antp),Ultrabithorax(Ubx),abdominalA(abdA),andAbdominalB(AbdB).ThemembersofHoxclusterareexpressedinadistinctanteriortoposteriororderintheembryo.AnalysisoftherelatednessofdifferentmembersoftheHoxgeneclustertoeachotherinfourevolutionarilydiverseinsecttaxarevealedthatthelocipb/DfdandAbdB,whicharefarthestapartinlinkage,hadahighdegreeofevolutionaryrelatedness,indicatingthatpb/DfdtypeanteriorgenesandAbdBareclosesttotheancestralanteriorandposteriorHoxgenes,respectively.ThegreaterrelatednessofotherposteriorgenesUbxandabdAtothemoreanteriorgenessuchasAntpandScrsuggestedthattheyarosebygeneduplicationsinthemoreanteriormembersratherthantheposteriorAbdB.

简介：<正>Acutepromyelocyticleukaemia(APL)hasbeenattractingawideinterestfarbeyondhematologicalfieldinthelastdozenyearsduetothepresenceofspecificchromosometranlocationsandclinicalresponsibilitytoall-transretinoicacid(ATRA)bydifferentiationinductionaswellastoarsenictrioxide(ATO).Most(>95%)APLpatientscarryspecificchromosometranslocationt(15;17),whichleadstodiscoveryofPMLgeneonchromosome15.SuchatranslocationcausesthefusionofPMLtoretinoicacidreceptor-alpha