简介：DragforcesactingonSchlegel'sblackrockfishSebastesschlegeliwerestudied.AnewdragforcetransducerwasdesignedandusedtomeasurethewaterdragonSchlegel'sblackrockfishinaverticalrecirculatingflumetank.Fourteenindividualswereinvestigated,yieldingtwomeandragcoefficientsreferredtothecross-sectionalareaandvolume2/3respectivelyatwatervelocitiesrangingfrom0.3to1.0ms-1.ThedragcoefficientscanbeusedforestimatingthedragforcesactingonSebastesschlegeliinwater.

简介：Vasa,whichisaconservedmemberoftheDEAD-boxproteinfamily,playsanindispensableroleinprimordialgermcellproliferation.However,theexpressionofvasageneduringthereproductivecycleinovoviviparousfishhasnotbeendocumented.Inthisstudy,thefull-lengthsequenceofvasawasobtainedfromtheovaryofKoreanrockfish(Sebastesschlegeli)usingreversetranscription-PCRandrapidamplificationofcDNAends.TheVasawithamatureproteinof650aminoacidsshowedgreatesthomology(84%)withgiantgourami(Osphronemusgoramy)andPacificbluefintuna(Thunnusorientalis).TheexpressionofvasamRNAinKoreanrockfishwasdetectedingonadsonly,suggestingitsspecificroleingonadaldevelopment.Inaddition,seasonalchangesinthevasaexpressionlevelswereexaminedingonadsbyquantitativereal-timePCR.Thevasatranscriptlevelsinadulttestiswerefoundhigherduringspermatogenesisthanduringspermiation.Thevasatranscriptlevelsremainedrelativelyhighattheearlyovarystagebutdeclinedduringovarymaturationinadultfemalefish.TheseresultssuggestthatthevasageneplayanimportantroleinspermatogenesisandearlyoogenesisduringthereproductivecycleofKoreanrockfish.

简介：EvolutionaryevidencesuggeststhatSox3,amemberofthehigh-mobility-group(HMG)familyoftranscriptionfactors,isanancestralprecursorofSryandisinvolvedinsexdeterminationsimilartoSry.However,thereislimitedinformationregardingtheSOX3geneoftheblackrockfish(Sebastesschlegeli).Inthisstudy,wefirstisolatedSOX3genefromthegonadsofS.schlegelibyhomologycloning.Thefull-lengthofS.schlegeliSOX3(SsSOX3)cDNAwas1386bp,comprisinga906-bpopenreadingframe,whichencodesapeptideshowing93.6%and93.9%homologywiththeSox3proteinsofEpinepheluscoioidesandOryziaslatipe,respectively.ComparisonofthecDNAsequenceoftheSsSOX3genewiththecorrespondinggenomicDNAfragmentrevealedthattheSsSOX3geneconsistsofasingleexon.PhylogeneticanalysisdemonstratedtheevolutionaryrelationshipofSsSOX3withotherknownSOXB1genesinfishandtetrapods.ThepromoterregioncontainsbindingsitesofseveraltranscriptionalfactorsthatmightparticipateintheregulationofSsSOX3expression.Quantitativereal-timePCRanalysisindicatedthatSsSOX3wasexpressedinalltheinvestigatedlarvaldevelopmentalstagesfrom1to35daysafterbirthandthelevelofexpressiongraduallydecreasedasthedevelopmentproceeded.SsSOX3exhibitedsexuallydimorphicexpressioninadultgonads,withhighexpressionintheovarybutlowexpressioninthetestis.InsituhybridizationrevealedthatSsSOX3wasstronglyexpressedinoocytesandfollicularcellsofovariesbutslightlyexpressedingermcellsoftesticulartissues.Therefore,thisstudysuggeststhatSsSOX3playsanimportantroleinoogenesisandovarydifferentiationinS.schlegeli.