简介：现在的学习被设计决定Pinelliapedatisecta的茎tuber的化学成分。化学成分被各种各样的色析法的技术，和他们的结构孤立并且净化根据物理化学的性质被阐明并且光谱数据。三新碱(加重1，2，和3)作为9-被获得并且识别((5-methoxypyridin-2-yl)甲基)-9H-purin-6-amine(1)，4-(2-(2，5-dioxopyrrolidin-1-yl)乙醇)苯基醋酸盐(2)，并且N-(9-((5-methoxypyridin-2-yl)甲基)-9H-purin-6-yl)acetamide(3)。这些混合物与人的颈的癌症HeLa房间为他们的cytotoxicity被作比较。混合物1和3显著地与是的IC50价值禁止了HeLa房间的增长3.02???????┱??????????????愠摮潲牧灡潨楬敤？？

简介：Consideringthegreatpotentialofnaturalproductsasanticanceragents,thepresentstudywasdesignedtoexplorethemolecularmechanismsresponsibleforanticanceractivitiesofMesuaferreastembarkextractagainsthumancolorectalcarcinoma.BasedonMTTassayresults,bioactivesub-fraction(SF-3)wasselectedforfurtherstudiesusingHCT116cells.Repeatedcolumnchromatographyresultedinisolationoflessactiveα-amyrinfromSF-3,whichwasidentifiedandcharacterizedbyGC-MSandHPLCmethods.α-amyrinandbetulinicacidcontentsofSF-3weremeasuredbyHPLCmethods.Fluorescentassaysrevealedcharacteristicapoptoticfeatures,includingcellshrinkage,nuclearcondensation,andmarkeddecreaseinmitochondrialmembranepotentialinSF-3treatedcells.Inaddition,increasedlevelsofcaspases-9and-3/7levelswerealsoobservedinSF-3treatedcells.SF-3showedpromisingantimetastaticpropertiesinmultipleinvitroassays.Multi-pathwayanalysisrevealedsignificantdown-regulationofWNT,HIF-1α,andEGFRwithsimultaneousup-regulationofp53,Myc/Max,andTGF-βsignallingpathwaysinSF-3treatedcells.Inaddition,promisinggrowthinhibitoryeffectswereobservedinSF-3treatedHCT116tumourspheroids,whichgiveahintaboutinvivoantitumorefficacyofSF-3phytoconstituents.Inconclusion,theseresultsdemonstratedthatanticancereffectsofSF-3towardscoloncancerarethroughmodulationofmultiplemolecularpathways.