简介: 摘要目的:探讨标本放置温度、时间及凝血真空采血管对凝血检验的影响。方法: 2018年 3-8月收治体检健康志愿者 80例,应用 2种不同的凝血真空采血管采集标本进行凝血检验。甲组使用 BD凝血真空采血管,乙组使用国产抗凝管。分析不同时间、不同温度对凝血检验结果的影响。结果:两组凝血酶时间( TT)、纤维蛋白原( FIB)、活化部分凝血活酶时间( APTT)、血浆凝血酶原时间( PT)比较,差异无统计学意义( P>0.05) ;低温保存 8h后, TT、 APTT、 PT高于即时检查,差异有统计学意义( P<0.05) ;室温保存 4h后, TT、 PT、 APTT高于即时检查结果,差异有统计学意义( P<0.05) ;FIB在室温、低温保存以及即时检查中差异均无统计学意义( P>0.05)。结论:不同质量的真空采集血管会对检查结果造成一定影响,而保存时间、放置温度均会影响检查结果,为了确保检验的准确性,应选择合适的凝血真空采集管,并在 2h内完成检查。 关键词 凝血检验 ;真空采集血管 ;放置时间 ;放置温度 Objective: To investigate the effects of temperature, time and blood vessel collection on blood coagulation test. Methods: from March to August 2018, 80 healthy volunteers were admitted for physical examination. Two kinds of blood coagulation vacuum blood collection specimens were used for coagulation test. In group A, blood vessels were collected by BD coagulation vacuum, and in group B, anticoagulation tubes made in China were used. The influence of different time and temperature on coagulation test results was analyzed. Results: there was no significant difference in TT, FIB, APTT and Pt between the two groups (P > 0.05); after 8 hours of cryopreservation, TT, APTT and PT were higher than those of the immediate examination (P < 0.05); after 4 hours of room temperature preservation, TT, Pt and APTT were higher than those of the immediate examination (P < 0.05) There was no significant difference in FIB in room temperature, cryopreservation and instant examination (P > 0.05). Conclusion: different quality of vacuum collection vessels will affect the results of the examination, while the storage time and temperature will affect the results of the examination. In order to ensure the accuracy of the examination, we should select a suitable coagulation vacuum collection tube and complete the examination within 2 hours.