简介：Withinthenervoussystem,regenerationislimited,andthisisduetothesmallamountofneuralstemcells,theinhibitoryoriginofthestemcellnicheandoftentothedevelopmentofascarwhichconstitutesamechanicalbarrierfortheregeneration.Regardingtheseaspects,manyeffortshavebeendoneintheresearchofacellcomponentthatcombinedwithscaffoldsandgrowthfactorscouldbesuitablefornervousregenerationinregenerativemedicineapproaches.Autologousmesenchymalstemcellsrepresentnowadaystheidealcandidateforthisaim,thanktotheirmultipotencyandtotheiramountinsideadulttissues.However,issuesintheirharvesting,throughtheuseofinvasivetechniques,andproblemsinvolvedintheirageing,requiretheresearchofnewautologoussources.Tothispurpose,therecentdiscoveryofastemcellscomponentinteeth,andwhichderivefromneuralcrestcells,hascametothelightthepossibilityofusingdentalstemcellsinnervoussystemregeneration.Inthiswork,inordertogiveguidelinesontheuseofdentalstemcellsforneuralregeneration,webrieflyintroducetheconceptsofregenerationandregenerativemedicine,wethenfocustheattentiononodontogenesis,whichinvolvestheformationandthepresenceofastemcomponentindifferentpartsofteeth,andfinallywedescribesomeexperimentalapproacheswhichareexploitingdentalstemcellsforneuralstudies.

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简介：Stemcellshavetheremarkablepotentialtodevelopintomanydifferentcelltypes,essentiallywithoutlimittoreplenishothercellsaslongasthepersonoranimalisstillalive,offeringimmensehopeofcuringAlzheimer’sdisease,repairingdamagedspinalcords,treatingkidney,liverandlungdiseasesandmakingdamagedheartswhole.Untilrecently,scientistsprimarilyworkedwithtwokindsofstemcellsfromanimalsandhumans:embryonicstemcellsandnon-embryonic'somatic'or'adult'stemcells.Recentbreakthroughmakeitpossibletoconvertor'reprogram'specializedadultcellstoassumeastemstem-likecellswithdifferenttechnologies.Thereviewwillbrieflydiscusstherecentprogressesinthisarea.更多还原

简介：Asingeniousasnature'sinventionofmyelinsheathswithinthemammaliannervoussystemis,asfatalcanbedamagetothisspecializedlipidstructure.Long-termlossofelectricalinsulationandoffurthersupportivefunctionsmyelinprovidestoaxons,asseenindemyelinatingdiseasessuchasmultiplesclerosis(MS),leadstoneurodegenerationandresultsinprogressivedisabilities.Multiplelinesofevidencehavedemonstratedtheincreasinginabilityofoligodendrocyteprecursorcells(OPCs)toreplacelostoligodendrocytes(OLs)inordertorestorelostmyelin.Muchresearchhasbeendedicatedtorevealpotentialreasonsforthisregenerationdeficitbutdespitepromisingapproachesnoremyelination-promotingdrugshavesuccessfullybeendevelopedyet.InadditiontoOPCsneuralstemcellsoftheadultcentralnervoussystemalsoholdahighpotentialtogeneratemyelinatingOLs.Thereareatleasttwoneuralstemcellnichesinthebrain,thesubventricularzoneliningthelateralventriclesandthesubgranularzoneofthedentategyrus,andanadditionalsourceofneuralstemcellshasbeenlocatedinthecentralcanalofthespinalcord.Whileasubstantialbodyofliteraturehasdescribedtheirneurogeniccapacity,stilllittleisknownabouttheoligodendrogenicpotentialofthesecells,evenifsomeanimalstudieshaveprovidedproofoftheircontributiontoremyelination.Inthisreview,wesummarizeanddiscussthesestudies,takingintoaccountthedifferentniches,theheterogeneitywithinandbetweenstemcellnichesandpresentcurrentstrategiesofhowtopromotestemcell-mediatedmyelinrepair.

简介：BACKGROUND:Gliomaisthemostcommonintracranialtumorandhasapoorpatientprognosis.Thepresenceofbraintumorstemcellswasgraduallybeingunderstoodandrecognized,whichmightbebeneficialforthetreatmentofglioma.OBJECTIVE:Tousebibliometricindexestotrackstudyfocusesongliomastemcell,andtoinvestigatetherelationshipsamonggeographicorigin,impactfactors,andhighlycitedarticlesindexedinWebofScience.METHODS:AlistofcitationclassicsforgliomastemcellswasgeneratedbysearchingthedatabaseofWebofScience-Expandedusingtheterms"gliomastemcell"or"glioma,stemcell’"or"braintumorstemcell".Thetop63citedresearcharticleswhichwerecitedmorethan100timeswereretrievedbyreadingtheabstractorfulltextifneeded.Eacheligiblearticlewasreviewedforbasicinformationonsubjectcategories,countryoforigin,journals,authors,andsourceofjournals.Inclusivecriteria:(1)articlesinthefieldofgliomastemcellswhichwascitedmorethan100times;(2)fundamentalresearchonhumansoranimals,clinicaltrialsandcasereports;(3)researcharticle;(4)yearofpublication:1899-2012;and(5)citationdatabase:ScienceCitationIndex-Expanded.Exclusivecriteria:(1)articlesneedingtobemanuallysearchedoraccessedonlybytelephone;(2)unpublishedarticles;and(3)reviews,conferenceproceedings,aswellascorrectedpapers.RESULTS:Of2040articlespublished,the63top-citedarticleswerepublishedbetween1992and2010.Thenumberofcitationsrangedfrom100to1754,withameanof280citationsperarticle.Thesecitationclassicscamefromnineteencountries,ofwhich46articlescamefromtheUnitedStates.DukeUniversityandUniversityofCalifornia,SanFranciscoledthelistofclassicswithsevenpaperseach.The63top-citedarticleswerepublishedin28journals,predominantlyCancerResearchandCancerCell,followedbyCellStemCellandNature.CONCLUSION:Ourbibliometricanalysisprovidesahistoricalpersp

简介：Parthenogeneticembryonicstemcellshavepluripotentdifferentiationpotentials,akintofertilizedembryo-derivedembryonicstemcells.Theaimofthisstudywastocomparetheneuronaldifferentiationpotentialofparthenogeneticandfertilizedembryo-derivedembryonicstemcells.Beforedifferentiation,karyotypeanalysiswasperformed,withnormalkaryotypesdetectedinbothparthenogeneticandfertilizedembryo-derivedembryonicstemcells.SexchromosomeswereidentifiedasXX.Immunocytochemistryandquantitativereal-timePCRdetectedhighexpressionofthepluripotentgene,Oct4,atboththemRNAandproteinlevels,indicatingpluripotentdifferentiationpotentialofthetwoembryonicstemcellsubtypes.Embryonicstemcellswereinducedwithretinoicacidtoformembryoidbodies,andthendispersedintosinglecells.SinglecellsweredifferentiatedinN2differentiationmediumfor9days.Immunocytochemistryshowedparthenogeneticandfertilizedembryo-derivedembryonicstemcellsbothexpresstheneuronalcellmarkersnestin,βIII-tubulinandmyelinbasicprotein.Quantitativereal-timePCRfoundexpressionofneurogenesisrelatedgenes(Sox-1,Nestin,GABA,Pax6,Zic5andPitx1)inbothtypesofembryonicstemcells,andOct4expressionwassignificantlydecreased.NestinandPax6expressioninparthenogeneticembryonicstemcellswassignificantlyhigherthanthatinfertilizedembryo-derivedembryonicstemcells.Thus,ourexperimentalfindingsindicatethatparthenogeneticembryonicstemcellshavestrongerneuronaldifferentiationpotentialthanfertilizedembryo-derivedembryonicstemcells.

简介：OBJECTIVE:Theaimofthisstudywastoevaluatetheeffectivenessandsafetyofstemcelltransplantationforspinalcordinjury(SCI).DATASOURCES:PubMed,EMBASE,Cochrane,ChinaNationalKnowledgeInfrastructure,ChinaScienceandTechnologyJournal,Wanfang,andSinoMeddatabasesweresystematicallysearchedbycomputertoselectclinicalrandomizedcontrolledtrialsusingstemcelltransplantationtotreatSCI,publishedbetweeneachdatabaseinitiationandJuly2016.DATASELECTION:RandomizedcontrolledtrialscomparingstemcelltransplantationwithrehabilitationtreatmentforpatientswithSCI.Inclusioncriteria:(1)PatientswithSCIdiagnosedaccordingtotheAmericanSpinalInjuryAssociation(ASIA)InternationalstandardsforneurologicalclassificationofSCI;(2)patientswithSCIwhoreceivedonlystemcelltransplantationtherapyorstemcelltransplantationcombinedwithrehabilitationtherapy;(3)oneormoreofthefollowingoutcomesreported:outcomesconcerningneurologicalfunctionincludingsensoryfunctionandlocomotorfunction,activitiesofdailyliving,urinationfunctions,andseverityofSCIoradverseeffects.Studiescomprisingpatientswithcomplications,withoutfull-text,andpreclinicalanimalmodelswereexcluded.QualityoftheincludedstudieswasevaluatedusingtheCochraneriskofbiasassessmenttoolandRevManV5.3software,providedbytheCochraneCollaboration,wasusedtoperformstatisticalanalysis.OUTCOMEMEASURES:ASIAmotorscore,ASIAlighttouchscore,ASIApinprickscore,ASIAimpairmentscalegradingimprovementrate,activitiesofdailylivingscore,residualurinevolume,andadverseevents.RESULTS:Tenstudiescomprising377patientswereincludedintheanalysisandtheoverallriskofbiaswasrelativelylowlevel.Fourstudiesdidnotdetailhowrandomsequencesweregenerated,twostudiesdidnotclearlystatetheblindingoutcomeassessment,twostudieslackedblindingoutcomeassessment,onestudylackedfollow-upinformation,andfourstudiesc

简介：BACKGROUND:Transplantedmononuclearcell(MNC)ofumbilicalbloodcansurviveincentralnervoussystem(CNS)ofhostthroughbloodbrainbarrier,differentiateintonervercells,migratetodamagedsiteandintegratemorphologicalstrucghandfunctionwithnervecellsofhostsoastoimprovedeficienciesofsensatoryfunction,motorfunctionandcognitivefunctionandinfluenceonstrokesequela.OBJECTIVE:Toobservetheveintransplantationofhumanumbilicalcordbloodstemcells(HUCBSC)forimprovingneurologicalfunction,limbfuntionandactivityofdailylivingofpatientswithstrokeandevaluatethereliability.DESIGN:Self-controlledstudy.SETTING:DepartmentofNeurosurgery,theSecondPeople'sHospitalofZhengzhouCity;Red-crossedBloodCenterofHenanProvince;DepartmentofNeurosurgery,theFistAffiliatedHospitalofZhenzhouUniversity.PARTICIPANTS:Atotalof10patientswithstokesequelawereselectedfromDepartmentofCerebralSurgery,theSecondPeople'sHospitalofZhengzhouCityfromApriltoDecember2005.Therewere9malesand1femaleagedfrom35to75yearswiththemeanageof56years.AllofthemwerediagnosedwithCTandMRIexaminationandcoincidencewithdiagnosticcriteriaofstrokeestablishedbytheFourthNationalAcademicMeetingforCerebrovascularDisease.Allpatientsprovidedinformedconsent.METHODS:80-140mLumbilicalbloodoftermbirthofnewbornwasselectedhermeticallyandmaintainedinsterileplasticbag.Andthen,thebloodwascentrifugatedatthespeedof1500r/minfor30minutesat22℃inordertoseparateMNC,i.e.,HUCBSC.Inaddition,afterfinaldiagnosisduringhospitalization,strokepatientswereperfusedwithHUCBSCthroughsuperficialveinofbackofthehand.Eachpatientwasaveragelypenfusedwith6portionsofHUCBSC(cellularnumbers≥1×108/portion)andtheintervalbetweeneachportionwas1-7dayswiththemeanintervalof4days.MAINOUTCOMEMEASURES:①Neurologicalfunctionofstrokepatientswasevaluatedwithneurologicalfunctiondeficiency(NFD)befor

简介：BACKGROUND:Duetothelackofautografttransplantrejection,Schwanncells(SCs)canpromotetheproliferationofembryonicstemcellsandtheinductionofdopaminergicneurons.Mesencephalicstemcellscanbeinducedtoproducedopaminergicneurons.Thetherapeuticeffectsofco-graftsofSCsandneuralstemcells(NSCs)deservesfurtherstudyandverificationinParkinsoniananimalmodels.OBJECTIVE:ToinvestigatetheeffectsofSchwanncellsandmesencephalicNSCco-graftsinParkinsoniananimalmodelsonanimalbehaviorandhistology.DESIGN:Randomizedcontrolledexperiment.SETTING:FudanUniversity;InstituteofNeuroscience,ChineseAcademyofSciences.MATERIALS:ThefollowinganimalswereobtainedfromtheExperimentalAnimalCenter,ShanghaiInstituteforBiologicalScience,ChineseAcademyofSciences:5Sprague-Dawleyrats,embryonicday(E)13-16;16neonatalSprague-Dawleyrats,postnatalday1-3;and18adultSDratsofbothgenders.Animalexperimentationmetanimalethicalapproval.METHODS:TheexperimentwasperformedattheDepartmentofAnatomy,HistologyandEmbryology,ShanghaiMedicalCenter,FudanUniversityfromSeptember2005toJanuary2007.ThemesencephalicNSCswereobtainedfromthebrainsofSDratsatE13-16,andSCswereharvestedfromthesciaticnervesofneonatalratsatday1-3.Hemiparkinsonianrats(n=18)wereselectedfortransplantationafterestimatingrotationalbehaviorinresponsetoapomorphineandwererandomlyassignedtothreegroups:controlgroup,NSCgroup,andco-graftgroup.Therewere6ratsineachgroup.Eitherphosphatebufferedsaline(PBS),NSCs,orSCsplusNSCsweretransplantedintotherightneostriatumofParkinsonianrats,respectively.MAINOUTCOMEMEASURES:①Rotationalbehaviorwasinducedbyapomorphine(0.05mg/kg,i.p.)2,4,6,8,and10weeksaftertransplantation,andthenumberofrotationswerecounted.②Differentiationandsurvivalofdopaminergicneuronsintherightneostriatumwerequantifiedbytyrosinehydroxylaseimmu

简介：Similarlytootheradulttissues,ahierarchicalstructurehasbeenestablishedforthebrain,wherethedifferentiatedcelltypes(neurons,oligodendrocytesandastrocytes)aregeneratedfromprimaryprogenitorcells,knownastypeB

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简介：ExogenoussubstancePaccelerateswoundhealingindiabetes,butthemechanismremainspoorlyunderstood.Here,weestablishedaratmodelbyintraperitoneallyinjectingstreptozotocin.Fourwounds(1.8cmdiameter)weredrilledusingaself-madepunchontotheback,bilateraltothevertebralcolumn,andthentreatedusingamnioticmembranewithepidermalstemcellsand/orsubstanceParoundandinthemiddleofthewounds.Withthecombinedtreatmentthewound-healingratewas100%at14days.Withprolongedtime,typeIcollagencontentgraduallyincreased,yettypeIIIcollagencontentgraduallydiminished.Abundantproteingeneproduct9.5-andsubstanceP-immunoreactivenervefibersregenerated.Partialnervefiberendingsextendedtotheepidermis.ThetherapeuticeffectsofcombinedsubstancePandepidermalstemcellswerebetterthanwithamnioticmembraneandeitherfactoralone.OurresultssuggestthatthecombinationofsubstancePandepidermalstemcellseffectivelycontributestonerveregenerationandwoundhealingindiabeticrats.

简介：Theextracellularmatrix,whichincludescollagens,laminin,orfibronectin,playsanimportantroleinperipheralnerveregeneration.Recently,aSchwanncell-derivedextracellularmatrixwithclassicalbiomaterialwasusedtomimictheneuralniche.However,extensiveclinicaluseofSchwanncellsremainslimitedbecauseofthelimitedorigin,lossofanautologousnerve,andextendedinvitroculturetimes.Inthepresentstudy,humanumbilicalcord-derivedmesenchymalstemcells(hUCMSCs),whichareeasilyaccessibleandmoreproliferativethanSchwanncells,wereusedtoprepareanextracellularmatrix.WeidentifiedthemorphologyandfunctionofhUCMSCsandinvestigatedtheireffectonperipheralnerveregeneration.Comparedwithanon-coateddishtissueculture,thehUCMSC-derivedextracellularmatrixenhancedSchwanncellproliferation,upregulatedgeneandproteinexpressionlevelsofbrain-derivedneurotrophicfactor,glialcell-derivedneurotrophicfactor,andvascularendothelialgrowthfactorinSchwanncells,andenhancedneuriteoutgrowthfromdorsalrootganglionneurons.ThesefindingssuggestthatthehUCMSC-derivedextracellularmatrixpromotesperipheralnerverepairandcanbeusedasabasisfortherationaldesignofengineeredneuralniches.

简介：Tourette'ssyndromeistreatedbybehavioralorpharmacologicaltherapy.However,patientswithmalignantTourette'ssyndromealsoexhibitlife-threateningsymptoms,whichareunresponsivetoconservativetreatmentsorneurosurgicalprocedures,suchasdeepbrainstimulation.Inrecentyears,mesenchymalstemcells(MSCs)haveshowntherapeuticpotentialinmanyneurologicaldiseases.Therefore,thepresentstudyproposedtouseMSCtransplantationasanoveltherapyforTourette'ssyndrome.StereotypicbehaviorsinTourette'ssyndromeratsdecreasedsignificantlyat21daysafterhumanMSCstransplantationintothestriatum.ImmunohistochemistryanalysesrevealedsurvivaloftransplantedhumanMSCsanddifferentiationintoneuronsandastrocytesintheratbrain.ResultssuggestthatintrastriataltransplantationofhumanMSCscouldprovidetherapeuticpotentialforTourette'ssyndrome.

简介：BACKGROUND:Microgliaareverysensitivetoenvironmentalchanges,oftenbecomingactivatedbypathologicalconditions.Activatedmicrogliacanexertadualroleininjuryandrepairinvariousdiseasesofthecentralnervoussystem,includingcerebralischemia,Parkinson’sdisease,andAlzheimer’sdisease.OBJECTIVE:Animmortalmicroglialcellline,BV2,wastreatedwithvaryingconcentrationsoflipopolysaccharide(LPS)toinduceapathologicalsituation.Supernatantwasharvestedandincubatedwithbonemarrowmesenchymalstemcellsand,concomitantly,bonemarrowmesenchymalstemcelldifferentiationwasobserved.DESIGN:Acontrolledobservation,invitroexperiment.SETTING:DepartmentofNeurology,FirstAffiliatedHospitalofChinaMedicalUniversity.MATERIALS:Fivemale2–3-week-oldSpragueDawleyratswerepurchasedfromAnimalLaboratoryCenterofChinaMedicalUniversityandincludedinthisstudy.Theprotocolwasperformedinaccordancewithethicalguidelinesfortheuseandcareofanimals.ThemicroglialcelllineBV2wasproducedbyCellResearchInstituteofChineseAcademyofSciences.LPSwasproducedbySigmaCompany,USA.METHODS:ThisstudywasperformedintheCentralLaboratoryofChinaMedicalUniversityfromSeptember2006toMarch2007.Ratfemoralandtibialbonemarrowwascollectedforseparationandprimarycultureofbonemarrowmesenchymalstemcells.Bonemarrowmesenchymalstemcellculturesweredividedinto5groups:controlgroup,non-activatedgroup,aswellaslow-,medium-,andhigh-doseLPSgroups.Inthecontrolgroup,bonemarrowmesenchymalstemcellswereculturedwithDulbecco’smodifiedEagle’smedium(DMEM)supplementedwithfetalbovineserum(volumefraction0.1).Inthenon-activatedgroup,bonemarrowmesenchymalstemcellswereincubatedwithnon-activatedBV2supernatant.Inthelow-,medium-,andhigh-doseLPSgroups,bonemarrowmesenchymalstemcellswereincubatedwithLPS(0.01,0.1and1μg/L,respectively)-activatedBV2supernatant.MAIN

简介：Recentadvancesinstemcelltechnologieshaveopenednewavenuesforthetreatmentofanumberofdiseasesstilllackingeffectivetherapeuticoptions.Celltransplantationhasemergedasamongthemostpromisingclinicalinterventionfordisorderssuchasinjuries,diabetes,liver

简介：Excessivenoise,ototoxicdrugs,infections,autoimmunediseases,andagingcancauselossofspiralganglionneurons,leadingtopermanentsensorineuralhearinglossinmammals.Stemcellshavebeenconfirmedtobeabletodifferentiateintospiralganglionneurons.Littlehasbeenreportedonadiposetissue-derivedstemcells(ADSCs)forrepairofinjuredspiralganglionneurons.Inthisstudy,wehypothesizedthattransplantationofneuralinduced-humanADSCs(NI-hADSCs)canrepairtheinjuredspiralganglionneuronsinguineapigswithneomycin-inducedsensorineuralhearingloss.NI-hADSCswereinducedwithculturemediumcontainingbasicfibroblastgrowthfactorandforskolinandtheninjectedtotheinjuredcochleae.GuineapigsthatreceivedinjectionofHanks’balancedsaltsolutionintothecochleaewereusedascontrols.Hematoxylin-eosinstainingshowedthatat8weeksaftercelltransplantation,thenumberofsurvivingspiralganglionneuronsinthecelltransplantationgroupwassignificantlyincreasedthanthatinthecontrolgroup.Alsoat8weeksaftercelltransplantation,immunohistochemicalstainingshowedthatagreaternumberofNI-hADSCsinthespiralganglionsweredetectedinthecelltransplantationgroupthaninthecontrolgroup,andtheseNI-hADSCsexpressedneuronalmarkersneurofilamentproteinandmicrotubule-associatedprotein2.Within8weeksaftercelltransplantation,theguineapigsinthecelltransplantationgrouphadagraduallydecreasedauditorybrainstemresponsethreshold,whilethoseinthecontrolgrouphadalmostnoresponseto80dBofclicksorpuretoneburst.ThesefindingssuggestthatalargeamountofNI-hADSCsmigratedtothespiralganglions,survivedforaperiodoftime,repairedtheinjuredspiralganglioncells,andtherebycontributedtotherecoveryofsensorineuralhearinglossinguineapigs.

简介：Traumaticinjuriestospinalcordelicitdiversesignalingpathwaysleadingtounselectiveandcomplexpathologicaloutcomes:deathofmultipleclassesofneuralcells,formationofcysticcavitiesandglialscars,disruptionofaxonalconnections,anddemyelinationofsparedaxons,allofwhichcancontributemoreorlesstodebilitatingfunctionalimpairmentsfoundinpatientswithspinal

简介：BACKGROUND:Culturesfrommultipleportionsofumbilicalcordbloodmesenchymalstemcellshavebeenshowntoundergomorerapidproliferationandattachmentthansingleportions.OBJECTIVE:Toobservegrowthofbasicfibroblastgrowthfactor(bFGF)-inducedculturesofhumanamnion-derivedmesenchymalstemcells(AMSCs)anddifferentiationintoneuronal-likecells.DESIGN,TIMEANDSETTING:Comparativeobservation.ThestudywasperformedattheLaboratoryofMicrobiologyandImmunology,BasicMedicalSchoolofZhengzhouUniversityfromJanuarytoMay2008.METHODS:Amniafromfull-term,uterine-incisiondeliveryweredonatedby12healthywomen.AMSCswereobtainedbycellseparationandculturetechniques,andwerepassagedandinducedbybFGF.Fromthethirdpassage,atotalof1mLAMSCs,atadensityof1.0×10~4/mL,wasseparatelyharvestedfromsixsamples,whichservedasgroupA.Atotalof1mLAMSCs,atadensityof1.0×10~4/mL,washarvestedseparatelyfromtheremainingsixsamples,whichservedasgroupB.Atotalof0.5mLfromthesixsamplesofgroupAand0.5mLfromthesixsamplesofgroupBwerecombinedtoformgroupC.MAINOUTCOMEMEASURES:Differencesincellquantityamongthethreegroupswerecomparedbycellquantificationand3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)analysis.Expressionofaglialcellmarker,neuron-specificenolase,andnestinwasdetectedinthethreegroupsbyimmunocytochemistry.RESULTS:CellquantificationandMTTanalysisoflivecells,aswellasAMSCabsorbance,weresignificantlygreateringroupCcomparedwithgroupsAandBat18daysofculture(P<0.05),andnosignificantdifferencewasobservedbetweengroupsAandB.Glialfibrillaryacidicprotein,neuron-specificenolase,andnestinwereexpressedinallgroupsfollowingbFGFinduction.CONCLUSION:MixedAMSCculturespromotedproliferation,andbFGF-inducedAMSCsdifferentiatedintoneuronal-likecells.

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