简介:YoungWistarratsweredividedintosixgroups,theexperimentalgroupsweregivenLa(NO3)3atdoseof20,10,2,0.2,0.1mg*kg-1andthecontrolgroupwasgivenphysiologicalsalinerespectivelyforsixmonths.Theanimalswereweighedandtheratiosofthelivertobodyweightwerecounted.Pathologicalchangesofliverwereobservedbylightmicroscopeandtransmissionelectronmicroscope.Glutamic-oxalacetictransaminase(GOT),glutamic-pyruvictransaminase(GPT),gamma-glutamyltransferase(γ-GT)andalklinephosphatase(ALP)intheserumweremeasured.Theresultsindicatethatthebodyweightofanimalsgainesslowlyinthegroupof20mg*kg-1La(NO3)3,butitgainedquicklyintheratsfedwith0.1mg*kg-1La(NO3)3.Biochemicalindexeshavenoabnormalchanges.Inthegroupof20mg*kg-1La(NO3)3,therewerelipiddropletsanddecreaseofglycogeninthehepatocytes,densermatrixofthemitochondria,deformationofthenucleiofsomehepatocytestodifferentdegreeandinfiltrationofinflammatorycellsatportalarea.ThemorethedoseofLa(NO3)3weregiventotherats,themorethenumberofbodiescontaininghighlyelectronicdensegravel-likegranulesandthesecondarylysosomeswithdensebodies.Theratsfedwith20mg*kg-1La(NO3)3forsixmonthsshowsinjuriouseffectsonthehepatocytes.
简介:ExperimentswerecarriedoutonavegetablefieldwithPekingcabbage(Brassicapekinensis(Lour.)Rupr.),cabbage(Brassicachinensisvar.OleiferaMakinoandnemoto),greencabbage(BrassicachinensisL.),spinach(SpinaciaoleraceaL.)andrape(BrassicacampestrisL.)tostudytheeffectsofNformsandNratesontheirgrowthandnitrateaccumulation.Theresultsindicatedthatapplicationofammoniumchloride,ammoniumnitrate,sodiumnitrateandureasignificantlyincreasedtheyieldsandnitrateconcentrationsofPekingcabbageandspinach.Althoughnosignificantdifferencewasfoundintheyieldsafterapplicationofthe4Nforms,nitrateNincreasednitrateaccumulationinvegetablesmuchmorethanammoniumN.ThevegetableyieldswerenotincreasedcontinuouslywithNrateincrease,andoversupplyofNreducedtheplantgrowth,leadingtoayielddecline.Thistrendwasalsotruefornitrateconcentrationsinsomevegetablesandatsomesamplingtimes.However,asawhole,thenitrateconcentrationsinvegetableswerepositivelycorrelatedwithNrates.Thus,additionofNfertilizertosoilwasthemajorcauseforincreasesinnitrateconcentrationsinvegetables.Nitrateconcentrationsweremuchhigherinroots,stemsandpetiolesthaninbladesatanyNrate.
简介:Theeffectofbicarbonate(HCO3-)onthegrowthanddevelopmentofplantsvariesbyspecies.Tobetterunderstandinorganiccarbonandnitrogenassimilationchangesofkarst-adaptableplantsunderdifferentHCO3-treatments,weconductedexperimentsonseedlingsandinvitroplantletsofOrychophragmusviolaceus(Ov).Wefoundthatthevitalphotosynthesispotential(asmeasuredbynetphotosyntheticrate,actualphotochemicalefficiencyofphotosystem-Ⅱ,photochemicalquenchingcoefficient,andtheinstantaneouscarbonisotoperatioof3-phosphoglycerate)wasconsistentunderdifferentHCO3-treatmentsofOv.Bicarbonate’slackofeffectoncarbonassimilationofOvmayberelatedtocarbonicanhydraseinOvconvertingHCO3-toH2OandCO2.Inthisway,OvcouldpreventHCO3-iontoxicityandhighpHfromharmingitsgrowthanddevelopmentunderHCO3-stress.ThisstudyalsofoundthathighHCO3-concentrationscouldpromotenitrogenassimilationandutilizationofOvthroughchangesinrelatedindexes(foliarnitrogenisotopefractionationratio,stablenitrogenisotopeassimilationratio,foliarstablenitrogenisotopefractionation,nitratenitrogenutilizationefficiency,andnitrateutilizationshare)underdifferentHCO3-treatments.BicarbonatehasdifferenteffectsonphotosynthesisandoninorganicnitrogenassimilationofOv,whichmaybeconnectedtophotosynthesisprovidingelectronsfornitrate/nitritereductionthroughthephotosyntheticchain.
简介:During1985~1987,theconcentrationofnitratenitrogenwashigherintheLaizhouBayandtheBohaiBaywhilethatofnitritenitrogenwashigherintheLiaodongBayandtheBohaiBay,Theconcentrationofnitratenitrogenwashighestinwinterandlowestinsummerwhilethatofnitritenitrogenwashighestinautumnandlowestinspring.theseasonalvariationoftheconcentrationofnitratenitrogenwasmaximumintheLaizhouBayandtheBohaiBaywhilethatoftheconcentrationofnitritenitrogenwasmaximumintheLiaodongBay.Therewasagreatdifferenceintheconcentrationofnitratenitrogenbetweenthesurfaceandthebottominautumnandintheconcentrationofnitritenitrogenbetweenthesurfaceandthebottominsummer.Themainreasonfortheseasonalvariationsoftheconcentrationofnitratenitrogenandnitritenitrogenwasthemarinebiochemicalprocess.ThenitratenitrogenandnitritenitrogenintheBohaiSeabasicallymaintainedaquasi-equilibriumstateseasonalcycle,Thequesi-equilibriumstateseasonalcycleofnitratenitrogenandnitritenitrogenatthebottomwasstablewhilethatatthesurfacewasliabletovariationscausedbyotherfactors.
简介:Aconductometricnitratebiosensorbasedonmethylviologen/Nafion/nitratereductase(NR)interdigitatedelectrodeforheavymetaldeterminationwasproposed.TheactivityofNR(EC1.6.6.2)fromAspergillusniger(A.niger)wasassayedasafunctionofmetalconcentrationinthepresenceofCu2+,Zn2+,Cd2+andPb2+.Parametersinfluencingtheperformanceofthebiosensorwereoptimizedfortheapplicationofitintheinhibitiondeterminationofheavymetalions.DetectionlimitsforCu2+,Zn2+,Cd2+andPb2+wereabout0.05,0.5,0.1and1.0μmol/L,respectively.TheresultsshowthatNRactivitycouldnotberegainedafterexposuretoCu2+,butcouldbepartiallyrecoveredafterexposuretoZn2+,Pb2+andCd2+.
简介:Nitratereductaseactivity(NRA)indifferentplantorgansandleavesindifferentpositionsofCamptothecaacuminataseedlingswasdeterminedbyanInvivoassay,thediurnalvariationrhythmofNRAinleavesofdifferentpositionswasobserved,andthecorrelationsbetweenleafNRA,leafareaandlaminamassperunitarea(LMA)werealsoexamined.TheresultsshowedthatNRAintheleafwassignificantlyhighest,comparedwiththatinotherorganssuchasroots,stemsandleaves.Inthisexperiment,the10leaveswereselectedfromtheapextothebaseoftheseedlingsinorder.ThedifferentNRAoccurredobviouslyinleavesofdifferentpositionsofC.acuminataseedlingsfromtheapextothebase,andNRAwashigherinthe4th-6thleaves.ThediurnalchangerhythmofleafNRAshowedaonepeakcurve,andmaximumNRAvalueappearedataboutmidday(at12:30orso).NoobviouscorrelationsbetweenNRAandleafareaorlaminamassperunitareawereobserved.ThisstudyofferedscientificfoundationforthefurtherresearchonnitrogenmetabolismofC.acuminata.
简介:Theeffectofnitrate(NO3-)onrice(OryzasativaL.)growthaswellasNabsorptionandassimilationduringdifferentgrowthstageswasexaminedusingthreetypicalricecultivars.Dryweight,yield,Nuptake,nitratereductaseactivity(NRA)inleaves,andglutaminesynthetaseactivity(GSA)inrootsandleavesduringtheirentiregrowthperiods,aswellasthekineticparametersofammonium(NH4+)uptakeattheseedlingstage,weremeasuredwithsolutioncultureexperiments.ResultsindicatedthatadditionofNH4+-NandNO3--Nataratioof75:25(NH4++NO3-treatment)whencomparedwiththatofNH4+-Nalone(NH4+treatment)increasedthedryweightof'Nanguang'cultivarby30%and'Yunjing38'cultivarby31%,andalsoincreasedtheirgrainyieldby21%and17%,respectively.Forthefourgrowthstages,thetotalNaccumulationinplantsincreasedbyanaverageof36%for'Nanguang'and31%for'Yunjing38',whereastheincreasingeffectofNO3-inthe'4007'cultivarwasonlyfoundattheseedlingstage.IntheNH4++NO3-treatmentcomparedtotheNH4+treatment,NRAintheleavesincreasedby2.09folds,andGSAincreasedby92%intherootsand52%intheleavesofthethreecultivars.NO3-supplyincreasedthemaximumuptakerate(Vmax)inthe'Nanguang'and'Yunjing38'cultivars,reflectingthattheNO3-itself,nottheincreasingNconcentration,increasedtheuptakerateofNH4+byrice.TherewasnoeffectontheapparentMichaelis-Mentenconstant(Km)ofthethreecultivars.Thus,somereplacementofNH4+withNO3-couldgreatlyimprovethegrowthofriceplants,mainlyonaccountoftheincreaseduptakeofNH4+promotedbyNO3-,andfuturestudiesshouldfocusonthemolecularmechanismoftheincreaseduptakeofNH4+byNO3-.
简介:Longaluminafiberswith60cminlengthwerepreparedbysol-gelmethod.Thespinningsolwasobtainedbymixingaluminumnitrate,oxalicacid,andpolyvinylpyrrolidonewithamassratioof10∶3∶2.Fouriertransforminfrared(FTIR)spectra,X-raydiffraction(XRD),andscanningelectronmicroscopy(SEM)wereusedtocharacterizethepropertiesofthegelandceramicfibers.Thefiberswithuniformdiameterandsmoothsurfacewereobtainedbysinteringat1200℃for1h,andtheirmainphasewasindentifiedasα-Al2O3.
简介:ThesolventextractionofPr(Ⅲ)andSm(Ⅲ)withtrialkylphosphineoxide(Cyanex923)inchloroform,carbontetrachloride,n-octane,cyclohexane,1,2-dichloroethane,benzene,toluene,xyleneandnitrobenzenefromacidicnitratemediumwasinvestigated.Onthebasisofslopeanalysisdata,thecompositionoftheextractedspecieswasdeterminedas(M(NO3)3?2CY923),CY923referstoCyanex923.Thevaluesoftheequilibriumconstantsandtheseparationfactorswerecalculated.Theeffectofthediluentsonthemetalextracti...
简介:Elevennitratenon-utilizing(nit)mutantswererecoveredfromsixisolatesofMagnaporthegriseaculturedonMMmedia,amendedwith60g/Lpotassiumchlorate,withafrequencyof1.42°.Somebiologicalproperties,suchasgrowthrate,growthbiomass,culturalcharacters,conidialproduction,sexualreproductionability,andpathogenicitywerecomparedbetweennitmutantsandtheirparentisolates.Resultsshowedthatallthenitmutantswereresistanttochlorate.SomeimportantbiologicalpropertiessuchasthegrowthrateonYPSA,conidialproductionabilityonTPSA,pathogenicity,hadnosignificantdifferencesbetweennitmutantsandtheirparentisolates.Matingtypedidn'tchange,butperitheciaproductionabilityoffertileisolateschangedsignificantlyascomparedwiththatoftheirparentisolates.Therefore.thenitcanbeusedasageneticmarkertostudythegeneticssuchaspathogenicity,fungicideresistanceinMagnaporthegrisea.
简介:印射土壤硝酸盐氮(NO3-N)的空间分发是重要的指导氮申请以及估计沥滤进地下水的NO3-N的环境风险。我们采用了univariate和混合geostatistical方法在东北佛罗里达越过一处风景印射土壤NO3-N的空间分发。土壤样品从四深度增长被收集(030,3060,60120和120180厘米)基于土壤,土地使用和举起阶层从用成层的随机识别并且嵌套采样的147个采样地点设计。在二层是的顶的土壤NO3-N分布空间地,汽车相关并且印射使用木头正常kriging。为NO3-N预言的环境关联模型用线性、非线性的回归方法被导出,并且采用了开发NO3-N趋势地图。陆地使用和它从卫星形象导出的相关变量作为重要变量被识别用环境关联模型预言NO3-N。当木头正常kriging生产了顺利变化的地图时,从环境关联模型导出的趋势地图产生了空间地异构的地图。趋势地图与趋势模型剩余的平常的kriging预言被相结合开发回归kriging预言地图,它给了最好的NO3-N预言。因为土地使用和遥远地察觉到的数据是容易地可得到的并且有大部分,与地相比的更好的空间分辨率取样了土壤,我们的调查结果基于为风景的使用和遥远地察觉到的数据放大土壤NO3-N印射的土地建议了环境关联模型的功效。实现的方法论为在另外的风景土壤NO3-N印射是可转让的。
简介:narG基因经常为细菌的减少硝酸盐的社区分析被用作一个分子的标记。在这研究,指向narG基因的教材的一个新集合被设计并且把使中毒反应的坡度胶化电气泳动(PCR-DGGE)试金用于嵌套半的聚合酶链。新教材的潜力在直接从在中央、南部的意大利散布的五个不同试验性的地点从土壤提取的DNA上被验证。教材的特性被切除决定,扩大,并且乐队定序由DGGE解决了。种系发生的分析显示出在从学习的土壤检索的序列和narG序列之间的关联从并且-Proteobacteria。这些教材扩展了存在为在土壤的减少硝酸盐的细菌的社区的尺寸和差异上的生态的学习的分子的工具。
简介:为了减少沥滤的硝酸盐,在土豆(茄属tuberosumL.)以后冒险收获并且改进氮化肥使用效率,一棵土豆卷心菜两倍收割系统(DCS)在Hetao被建立,诺思中国,有灌溉土地的一个干旱区域。一个二年的地实验证明在塑料林地覆盖物下面种早成熟的土豆栽培变种弄短它由14d的生长时期并且允许卷心菜的第二庄稼清除土壤剩余NO3S-N到160厘米的深度,实质地减少硝酸盐沥滤进地下水的风险。在DCS的每年全部的N举起在常规收割系统(CCS)是多于那的大约110kgha1,即,种的单音的土豆。这说明了明显的氮恢复(ANR)在DCS的16.90%26.57%的改进作为与那相比在为两年的CCS。作为结果,在在DCS的0160厘米土壤侧面的土壤剩余NO3-N在CCS是比那低的。太阳的精力使用效率和土壤使用效率实质地也与DCS被增加。
简介:Thetransportandchemicalproductionprocessesofnitrate,sulfate,andammoniumaerosolsoverEastAsiawereinvestigatedbyuseoftheModels-3CommunityMulti-scaleAirQuality(CMAQ)modelingsystemcoupledwiththeRegionalAtmosphericModelingSystem(RAMS).Fortheevaluationofthemodel'sabilityindepictingtheir3-dimensionalconcentrationdistributionsandtemporalvariations,modeledconcentrationsofnitrate,sulfate,andammoniumaerosolsarecomparedwiththeobservationsobtainedatagroundstationinJapaninMarch2001andonboardofanaircraftDC-8on18and21March2001duringtheTransportandChemicalEvolutionoverthePacific(TRACE-P)fieldcampaign.Comparisonshowsthatsimulatedvaluesofnitrate,sulfate,andammoniumaerosolsaregenerallyingoodagreementwiththeirobserveddata,andthemodelcapturesmostimportantobservedfeatures,andreproducestemporalandspatialvariationsofnitrate,sulfate,andammoniumaerosolconcentrationsreasonablywell,e.g.,thetimingandlocationsoftheconcentrationspikesofnitrate,sulfate,andammoniumaerosolsarewellreproduced,butlargediscrepanciesbetweenobservedandsimulatedvaluesarealsoclearlyseenatsomepointsandsometimesduetothecoarsegridresolutionanduncertaintiesoftheemissionsusedinthisstudy.ThiscomparisonresultsindicatethatCMAQisabletosimulatethedistributionsofnitrate,sulfate,andammoniumaerosolsandtheirrelatedspeciesinthetroposphereoverEastAsiareasonablywell.