简介：<正>ThesusceptibilityofprimaryBcellstoFas(APO-1,CD95)-mediatedapoptosisisregulatedbysignalsderivedfromadditionalsurfacereceptors.CD40engagementproducesupregulationofFasexpressionandinducesmarkedsensitivitytoFas-inducedcelldeath,whereasBcellantigenreceptor(BCR)engagementinhibitsFaskillingandtherebyproducesFas-resistance,eveninotherwisesusceptible,CD40-stimulatedtargets.BCRsignalingforinducibleFas-resistancedevelopsoveraperiodof12hoursanddependson

简介：热吃惊(HS)表明的在transducing的肌醇1,4,5-trisphosphate(IP(3))的角色在Arabidopsis被检验。整个植物的IP(3)水平在37度C在HS的1min以内增加了。在HS的3min以后，IP(3)水平到达了最大值2.5褶层增加。用有AtHsp18.2promoter-beta-glucuronidase(GUS)的转基因的Arabidopsis植物熔化基因，GUS活动的水平在non-HS和HS温度由将关入笼中的IP(3)的增加是起来调整的并且由phospholipaseC(PLC)禁止者是下面调整的，这被发现{1-[6-((17beta-3-Methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-2,5-pyrrolidinedione}(U-73122)。细胞内部免费的钙离子集中([Ca(2+)](i))在表示apoaequorin的暂停有教养的Arabidopsis房间在37度C在HS期间增加了。有U-73122的处理阻止了增加[Ca(2+)](i)到某程度。上面的结果在更高的植物在HS信号transduction为IP(3)的可能的参与提供了主要证据。

简介：Themethodoflasercapturemicrodissection(LCM)combinedwithsuppressivesubtractivehybridization(SSH)wasdevelopedtoisolatespecificgermcellsfromhumantestissectionsandtoidentifythegenesexpressedduringdifferentiationanddevelopment.Inthepresentstudy,over10,000primaryspermatocytesandroundspermatidcellsweresuccessfullyisolatedbyLCM.UsingthecDNAsfromprimaryspermatocytesandroundspermatids,SSHcDNAslibraryofprimaryspermatocyte-specificwasconstructed.TheaverageinsertsizeofthecDNAisolatedfrom75randomlypickedwhitecloneswas500bp,rangingfrom250bpto1.7kb.Usingthedot-blotmethod,atotalof421cloneswereexamined,resultingintheidentificationof390positiveclonesemittingstrongsignals.PartialsequenceofcDNAspreparedfromeachclonewasdeterminedwithanoverallsuccessrateof84.4%.GenesencodingcytochromecoxidaseⅡandtherescuefactor-humaninweremostfrequentlyexpressedinprimaryspermatocytes,suggestingtheirrolesinvolvedinmeiosis.