Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells-中国期刊网

摘要 ShortinterferingRNA(siRNA)iswidelyusedforstudyingpost-transcriptionalgenesilencingandholdsgreatpromiseasatoolforbothidentifyingfunctionofnovelgenesandvalidatingdrugtargets.TwosiRNAfragments(siRNA-aand-b),whichweredesignedagainstdifferentspecificareasofcodingregionofthesametargetgreenfluorescentprotein(GFP)gene,wereusedtosilenceGFPexpressioninculturedgfptransgeniccellsofrice(OryzasativaL.;OS),cotton(GossypiumhirsutumL.;GH),Fraserfir[Abiesfraseri(Pursh)Poir;AF],andVirginiapine(PinusvirginianaMill.;PV).DifferentialgenesilencingwasobservedinthebombardedtransgeniccellsbetweentwosiRNAs,andtheseresultswereconsistentwiththeinactivationofGFPconfirmedbylaserscanningmicroscopy,Northernblot,andsiRNAanalysisintestedtransgeniccellcultures.ThesedatasuggestthatsiRNA-mediatedgeneinactivationcanbethesiRNAspecificindifferentplantspecies.TheseresultsindicatethatsiRNAisahighlyspecifictoolfortargetedgeneknockdownandforestablishingsiRNA-mediatedgenesilencing,whichcouldbeareliableapproachforlarge-scalescreeningofgenefunctionanddrugtargetvalidation.

1WeiTang HilaryCollver KatherineKinken. Dexamethasone-Inducible Green Fluorescent Protein Gene Expression in Transgenic Plant Cells.分子生物学,2004-01.
2MIAOJUNYING;SATOHIKOARAKI;等. SHORT COMMINICATION——Involvement of gene expressions in apoptosis of vascular endothelial cells induced by rattlesnake venom.细胞生物学,1999-03.
3季加孚;张霁;焦春雨;顾晋;谭立新;张平;李培详. POST-TRANSCRIPTIONAL REGULATION OF P21WAF1/CIP1 BY P53.肿瘤,2001-02.
4孙立军;黄强;兰青;杜子威;胡庚熙;王爱东. GENE EXPRESSION PROFILING OF PHENYLBUTYRATE INDUCED DIFFERENTIATION OF GLIOMA CELLS BY cDNA ARRAY.肿瘤,2003-01.
5Wu Ruqun Zhu Jiayun Huang Junjun Du Yarong Xu An Wu Lijun Hu Burong. 3 - 91 Difference of Radiation-induced Bystander Effects in Cells Cultured in Monolayer or Three Dimensional Levels.物理,2010-01.
6Zain Paroo;Qinghua Liu;Xiaodong Wang. Biochemical mechanisms of the RNA-induced silencing complex.细胞生物学,2007-03.
7Aili Li;Long Mao. Evolution of plant microRNA gene families.细胞生物学,2007-03.
8Shen;Songdong(沈颂东);Dai;Jixun(戴继勋). Isolated Cells of Porphyra yezoensis Cultured on Solid Medium.海洋科学,2001-01.
9. Irradiation Effects of Heavy Ionson Cultured Mammalian Cells.物理,1997-10.
10WANG Wei;Wu Chao;LIU Mei;LIU Xu-ri;Hu Guo-cheng;SI Hua-min;SUN Zong-xiu;LIU Wen-zhen;Fu Ya-ping. Resistance of Antimicrobial Peptide Gene Transgenic Rice to Bacterial Blight.作物学,2011-01.

Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells

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Post-transcriptional Gene Silencing Induced by Short Interfering RNAs in Cultured Transgenic Plant Cells

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