简介:AlantolactoneisanaturalcompoundidentifiedfromtherootsofInulaheleniumL.thathasmultiplebio-activities.Weexamineditsinhibitoryeffectsonhumannon-smallcelllungcancer(NSCLC)A549cells.Thean-tiproliferativeeffectofalantolactoneonA549cellswasinvestigatedviaMTT[3′-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide]assayanditsapoptosis-inducingeffectwasdeterminedbyHoechststainingandflowcytometry.WefoundthatalantolactonesignificantlyinhibitedtheproliferationofA549cellsandinducedmorphologicalchangestypicalforapoptosis.Flowcytometryanalysisindicatesdose-dependentcellcycleretardationatG0/G1andSstages.Theresultsindicatethatalantolactonecouldbeanattractivesmall-molecularnaturalcompoundforfurtherdevelopmentasatherapeuticdrugagainstNSCLC.
简介:CunanoclusterswereelectrochemicallydepositedonthefilmofaNafion-solubilizedmulti-wallcarbonnanotubes(CNTs)modifiedglassycarbonelectrode(CNTs-GCE),whichfabricatedaCu-CNTscompositesensor(Cu-CNTs-GCE)todetectglucosewithnon-enzyme.Thelinearrangeis7.0×10-7to3.5×10-3mol/Lwithahighsensitivityof17.76μA/(mmolL),withalowdetectionlimit2.1×10-7mol/L,fastresponsetime(within5s),goodreproducibilityandstability.
简介:Thenanoscalealuminumbowlswerederivedfromtheporousaluminaandwereusedastheflexiblenanoscalereactorsforthepreparationofnanoparticles.Bothsinglesourceprecursorandprepreparednanoparticleswereinducedinthenanobowlsbymeltingtheprecursor/polymerfilmsspin-coatedonaluminumnanobowlsfortheformationofnanostructuralcompositesinthenanobowls.Wehavepreparedasinglenanoparticleorjustasmallnumberofmetal(e.g.Pt)nanoparticlesorsemiconductornanoparticles(e.g.CdSeorCdSe/ZnScore-shellnanostructures)inthenanobowls.
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简介:Itwasfoundthatphosphorylationofsmallpeptidecouldimprovethesensitivityinmassspectrometry.DensityfunctionaltheorycalculationsshowedthattheenergyfortheprotonationofN-(O,O′-dimethyl)phosphorylglycylglycineislowerthanthatofglycylglycine.Thesecouldhelptounderstandingtheexperimentalresults.
简介:Basedonageneralclassificationandcharacteristiccomparisonoftheexistingmodels,anewmodelfornon-catalyticgas-solidreactionsisproposedandageneralformulationforthemodelintermsofthesolidconversion,X,ispresentedinthispaper.Themodel,referredtothegeneralizedmodel,isdemonstratedtobeapplicabletoanysolidreactantofgeneralstructurerangingfromhighlyporoustononporousmaterials.Itisshownthatthegeneralizedmodelincorporatesthegrainandporestructureforasolidpelletandcanbereducedtothegrainandrandomporemodelsasextremecases.
简介:Theauthorsfocusedtheirattentionontheestablishmentofamesenchymalstemcell(MSC)modelforscreeningtraditionalChinesemedicines(TCMs)soastoinvestigatetheeffectsofShuanglongFormula(SLF)components(Ginsenosidesandsalvianolicacids)andingredients(ginsenosideRb1andsalvianolicacidB)oncardiomyocytedifferentiationfromMSCs.TheSLFcomponentswereanalyzedandquantifiedbyHPLC-TOF-MS.CardiomyocytedifferentiationwasinducedbyculturingMSCsintheinductionmediumsupplementedwithSLFingredients,SLFcomponents,5-azacytidine(5-aza),5-aza+SLFingredientsand5-aza+SLFcomponents,respectively,forupto30d,andevulatedbytheexpressionofCardiac-specificmyosinheavychain(MHC)andtroponinI(TnI)viaimmunofluoresentstaining.Slowgrowthrateandchangedmorphologywereobservedduringcardiomyocytedifferentiation.After20dofinduction,differentiatingMSCswerepositiveforMHCandTnIstaining.TheeffectsofSLFcomponentswerebetterthanthoseofSLFingredients.Takentogether,SLFcaninducethedifferentiationofMSCsintocardiomyogeniccellsinvitro,andMSCscanbeusedasapowerfultoolforscreeningTCMs.
简介:Aseriesof5-aminolevulinicacidanditsalkylestermethanesulfonateswasexploitedtophotodynamictherapy(PDT)ofhumanlymphocyticcells,U-937invitro.ThePDTefficiencyisinfluencedbytheconcentrationandincubationtime.Generally,forALAanditsalkylestermethanesulfonates,thecellsurvivalratedecreasesandtheaccumulationabilityofPpIXincreaseswiththeconcentrationandincubationtime.Wefoundthatthelongercarbonchainmethanesulfonates(C5-S,C6-S,C8-S)exhibitbetterPDTeffectthanALAmethanesulfonate.ThispossiblyprovidesapromisingroutetotheclinicalapplicationofPpIX-mediatedPDTtocancercell.
简介:Twenty10-hydrocarbylacridonesand2-methylacridone,1-hydroxyacridoneweresynthesizedfromacridoneandcharacterizedbymp,IR,UV,1HNMRandMS.UsingNd:YAGasalasersource,thesecond-orderharmonicgeneration(SHG)valuesoftheacridonederivativesweremeasuredinpowderstateascomparedwithureapowder.TheresultsshowedthattheSHGvaluesofsomeof10-hydrocarbylacridoneswerehigherthanthatofurea,whileotherswerelower.Althoughthehydrocarbylsubstituents(R)attachedtonitrogenatomofacridoneweredifferentinsizeandelectronegativity,theyhadalittleeffectontheSHGvaluesof10-hydrocarbylacridones.Substituents,suchasmethylorhydroxygroupconnectedtothephenylring,causedalittleeffectontheSHGvalues,too,comparedwithacridone.TheabilityofRtopushelectrontowardthenitrogenatomortopullelectronfromthenitrogenatomplayanimportantroleonthemaximumwavelengthesofUV-visibleabsorptionofacridonederivatives.
简介:Insituphotochromicprocessinthemonolayerofaphotochromicspiropyranderivativewithoutlongalkylchain,wasinvestigated.Thephotochromismattheair/waterinterfaceunderdiffernetsurfacepressureswasstudiedbysurfacepressure-areaisotherms,surfacepressure-timecurves,area-timecurvesandBrewsteranglemicroscopy.Bothformsofthecompoundwerefoundtoformmonolayersattheair/waterinterfacealthouhgitdoesnothavelongalkylchain.Alargeareaexpansioninthemonolayercorrespondingtoazreo^thorderreactionwasfoundattheinitialstageoftheUVlightirradiation.Aseriesofdynamicinvestigationsrevealedthatathighpressureafterphasetransitioninthemonolayer,thesurfacepressurechangesgreatlyumderalternativeirradiationofUVandvisiblelight.Anobviousmorphologicalchangeaccompanyingwiththephotochromismwasobservedinsitu.
简介:Thelackofefficientandnon-toxicgenedelivery,preferablywithnon-viralDNAvectors,isgenerallyregardedasamajorlimitationforgenetherapy.Inthisstudy,awheathistoneH4genewasclonedfromTriticumaestivum,sequenced,modifiedandexpressedinE.coli.ThewheathistoneH4geneandreconstructedH4TLgeneencodedwheathistoneH4andarecombinantproteinof141aminoacidswithanapproximatemolecularweightof15500.GelelectrophoresismobilityshiftassaysdemonstratedthatthepurifiedproteinhadhighaffinityforDNA.Mostsignificantly,thecomplexofplasmidpEGFP/C1withH4TLwastransfectedwithincreasedefficiencyintoMCF-7,HO8910,LNCap,A549andHeLacellsinvitro.Theseresultsdemonstratethatthetargetingofnon-viralvectorstotumor-specificreceptorsprovidesacheap,simpleandhighlyefficienttoolforgenedelivery.