简介：Objective:Toinvestigatetherelationshipbetweengenemutationandpathologicaltypeoflungcancer,inspectandverifytheconsistencybetweenhomologousgenesmutationinvariouspathologictype.Methods:CombinedwiththeCOSMICandUniProtdatabase,weobtainedthereportedoverallbig-samplemutationdataoflungcancerandtheproteinsequencesofthetop20mutatedgenes,respectively.Analyzethedataandclustertheproteinsequencesandthendeducethehomologousgene.Ultimately,analyzethemutationsofdifferentpathologicaltypesofhomologousgenes.Results:TP53(32.32%)hasthehighestmutationrateinlungcancer,followedbyEGFR(29.12%).Thecopynumbervariability(CNV)ofgenes:KRAS,LRP1B,CDKN2A,KMT2C,FAT1,PIK3CA,RB1,ERBB4,GRIN2AandKDRbetweeneachpathologicaltypeisstaticallysignificant(P<0.05).ThegenedifferentialexpressionratebetweenadenocarcinomaandsquamouscarcinomaofgeneTP53,KRAS,LRP1B,CDKN2A,STK11,FAT4,KMT2D,NFE2L2,KEAP1,PIK3CA,RB1,ERBB4,SMARCA4andKDRarestatisticallysignificant(P<0.05).ThesimilarityoftheproteinsequenceofEGFRandERBB4canreach93%,andFAT4andFAT1are81%.Forsmallcellcarcinoma,there’snodifferenceinCNVbetweenthetwogroupsofhomologousgenes,andnodifferencebetweenFAT4andFAT1inadenocarcinoma.Conclusion:TheCNVandgeneexpressionoflungcancer-associatedgenesarerelevanttopathologictypes.GFRandERBB4arehomologous,FAT4andFAT1arealsoamongthetop20mutationgenes.Additionally,there’snodifferenceinCNVbetweenthetwogroupsofsmallcellcarcinoma,whichisthesamebetweenFAT4andFAT1inadenocarcinoma.

简介：ＥＦＦＥＣＴＳＯＦＡＰＡＴＩＴＥＣＥＲＡＭＩＣＳＯＮＣＥＬＬＧＲＯＷＴＨＳＡＮＤＤＮＡＳＹＮＴＨＥＳＥＳＩＮＨＵＭＡＮＯＳＴＥＯＢＬＡＳＴＬＩＫＥＣＥＬＬＥＦＦＥＣＴＳＯＦＡＰＡＴＩＴＥＣＥＲＡＭＩＣＳＯＮＣＥＬＬＧＲＯＷＴＨＳＡＮＤＤＮＡＳＹＮＴＨ...

简介：ＴＨＥＩＮＦＬＵＥＮＣＥＳＯＦＳＭＡＬＬＭＯＬＥＣＵＬＥＳＯＮＦＩＢＲＩＮＯＧＥＮＣＯＡＧＵＬＡＢＩＬＩＴＹＴＨＥＩＮＦＬＵＥＮＣＥＳＯＦＳＭＡＬＬＭＯＬＥＣＵＬＥＳＯＮＦＩＢＲＩＮＯＧＥＮＣＯＡＧＵＬＡＢＩＬＩＴＹＷａｎｇＣｈｕｎｒｅｎ；ＹａｎｇＺ...

简介：Redbloodcells(RBC)’flickeringpresentthedynamicpropertiesofthecytomembrane.Itscomplexitycouldbeusedforaginganalysisortheevaluationforthestoragequality.Theflickeringactivityisakindofreversibleperpendicularmotionofthespecifiedpixel.Therefore,thecomplexityanalysisdependsonthereliabledetectionoftemporalvariationforthegray-scalevaluesfromeachpixelofthecells.Inthispaper,weimprovedourpreviousworkonthescreeningofthehorizontaldriftedcellswithasurfacebasedoncellregistrationmethodandtheeffectofGSMexposuretothedynamicpropertiesoftheRBCsintermsofmulti-scalesampleentropywaspresentedinthepaper.

简介：Inordertounderstandthedevelopmentofstemcellsintospecializedmaturecellsitisnecessarytostudythegrowthofcellsinculture.Forthispurposeitisveryusefultohaveanefficientcomputerizedcelltrackingsystem.Inthispaperaprototypesystemfortrackingneuralstemcellsinasequenceofimagesisdescribed.Inordertogetreliabletrackingresultsitisimportanttohavegoodandrobustsegmentationofthecells.Toachievethiswehaveimplementedthreelevelsofsegmentation.Theprimarylevel,appliedtoallframes,isbasedonfuzzythresholdandwatershedsegmentationofafuzzygrayweighteddistancetransformedimage.Thesecondlevel,appliedtodifficultframeswherethefirstalgorithmseemstohavefailed,isbasedonafastgeometricactivecontourmodelbasedonthelevelsetalgorithm.Finally,theautomaticsegmentationresultonthecrucialfirstframecanbeinteractivelyinspectedandcorrected.Visualinspectionandcorrectioncanalsobeappliedtootherframesbutthisisgenerallynotneeded.Forthetrackingallcellsareclassifiedintoinactive,active,dividingandclusteredcells.Differentalgorithmsareusedtodealwiththedifferentcellcategories.Aspecialbacktrackingstepisusedtoautomaticallycorrectforsomecommonerrorsthatappearintheinitialforwardtrackingprocess.

简介：UsingFX-4000strainunit,theprolierationinhumanlungadenocarcinomaA549cellsthatunderwentmechanicalstrainofdifferentwaveform、frequencyanddurationwerestudied.Imageanalysisrevealedthatcellularproliferationrate(PR)reducedsignificantlyaftercellsweresubjectedtosquarewavewith0～20%elongationatfrequency30,40,50and60cycles/minfor2h.ThePRhadnodistinctdifferenceatheartwave,trianglewaveandsinewavegroupcomparedwithcontrol.ItisconcludedthatsquarewaveandhigherfrequencyplayanimportantroleininhibitingA549cellsproliferation.

简介：VanAndelResearchInstitute(VARI)investigatorsworkingincollaborationwithClevelandClinicresearchershavedeterminedthattwotypesofkidneytumorspreviouslythoughttobedifferentdiseasesareactuallyvariationsofthesamedisease.Thisfindingwillhelpdoctorstomoreaccuratelydiagnosethediseaseinpatients,anddemonstratestheimpor-tanceofusingmoleculardatatodiagnosecanceringeneral.

简介：AcomputerscientistatWashingtonUniversityinSt.Louishasdevelopedawaytocoaxcellstodonaturalthingsunderunnaturalcircumstances,whichcouldbeusefulforstemcellresearch,genetherapyandbiofuelproduction.MichaelBrent,theHenryEdwinSeverProfessorofEngineeringintheSchoolofEngineering&

简介：Rabbitlimbalcornealepithelialcells,cornealendothelialcellsandkeratocyteswereculturedonamnioticmembrane.Phasecontrastmicroscopeexaminationwasperformeddaily.Histologicalandscanelectronmicroscopicexaminationswerecarriedouttoobservethegrowth,arrangementandadhesionofcultivatedcells.Resultsshowedthatthreecornealcelltypesseededonamnioticmembranegrewwellandhadnormalcellmorphology.Culturedcellsattachedfirmlyonthesurfaceofamnioticmembrane.Cornealepithelialcellsshowedsingularlayerorstratification.Cellboundarieswereformedandtightlyopposed.Cornealendothelialcellsshowedcobblestoneorpolygonalmorphologiccharacteristicsthatappeareduniforminsize.Thecellulararrangementwascompact.Keratocyteselongatedandshowedtriangleordendriticmorphologywithmanyintercellularjointswhichcouldformnetworks.Inconclusion,amnioticmembranehasgoodscaffoldproperty,diffusioneffectandcompatibilitywithcornealcells.Thebasementmembranesideofamnioticmembranefacilitatedthegrowthofcornealepithelialcellsandendothelialcellsandcelljunctionsweretightlydeveloped.Thespongylayerofamnioticmembranefacilitatedthegrowthofkeratocytesandintercellularjointswererich.Amnioticmembraneisanidealbiomaterialforlayeringtissueengineeredcornea.

简介：Ananomeshhasbeendevelopedthatnearlytriplessolarcellefficiency.Theresearchteammanagedtoreducethereflectivityofthematerialsused,effectivelyincreasingtheamountofcapturedlight.Inadditiontothis,thenewsolarcellsarealsomuchthinnerthancurrentonesonthemarket.Thestructureiscreatedbysandwichingmetalwithnanomeshandisbeingcalled'PlasomnicCavity

简介：ＴＨＥＰＲＯＰＥＲＴＩＥＳＯＦＬＥＵＫＯＣＹＴＥＡＮＤＥＲＹＴＨＲＯＣＹＴＥＡＤＨＥＲＥＤＴＯＥＮＤＯＴＨＥＬＩＡＬＣＥＬＬＩＮＦＬＯＷＦＩＥＬＤＴＨＥＰＲＯＰＥＲＴＩＥＳＯＦＬＥＵＫＯＣＹＴＥＡＮＤＥＲＹＴＨＲＯＣＹＴＥＡＤＨＥＲＥＤＴＯＥＮＤＯＴ...

简介：Theapplicationofhumanhomografthasbeenpopularincardiovascularsurgeryandshownperfecteffects.Butthedifficultytogetgraftgreatlylimiteditsclinicalap-plication.Thethawedhomograftcannotbeusedinsomecasessinceitisnotclearwhetheritcanbere-cryopreservedwithoutdamage.Weconductedthepresentstudytoknowthechangesinviabilityofthehomograftafterrefreezinginsixhumanpul-

简介：Thispaperdevelopesatemperaturecontrolsysteminordertocarryoutarealtimeandcontinuousobservationonlivingcellsunderthemicroscope.Inthissystem,transparentindiumtinoxid(ITO)conductivefilmwasusedasaheater,temperaturewasmeasuredbythetemperaturedependenceofresistancebehavioroftheITOfilm,C8051F340single-clupwasusedasthecontrolhardwarecore,andthemodifiedPIDalgorithmandpulsewidthmodulation(PWM)wereadoptedasthekeycontrolsoftwareprograms.Thetemperaturesystemhadasimplestructurewithoutaddedtemperaturesensor,anuniformtemperaturedistributionwithin±1℃ontheITOfilmsurfaceandtemperaturecontrolprecisionof±0.2℃couldbeobtained.ThesystemcouldmaintainagoodgrowthstateforBHK-21livingcellunderthemicroscopefor48h.

简介：Inarecentstudy,researcherstookadultfemaleFischerratsandperformedaspinalcordtransectionontheminanattempttostudythegrowthoftransplantedearly-stageneurons.Whensuchearly-stageneuronsweretransplantedintoratssufferingfromparalysis,remarkableaxonalgrowthwasobserved.Theresultwasmanynewrelaycircuitsthatformed,whichsignificantlyimprovedfunction,

简介：

简介：Anewtechnologythatsimulatestumorshasbeenshowntoperformaswellasresearchanimalsintestingchemotherapydrugs,representingapotentialtoolforscreeningdrugsbeforetreatingapatient.Along-termgoalistoincorporatebiopsiedcancercellsfrompatientsandtesttheeffectivenessofdifferentdrugsonthepatient-derivedcells.

简介：Recentinterestinmobile-basedhealthcarehasdrivensignificantdemandsonresearchingnon-contactelectrodesforelectrocardiogram(ECG)measurement.Whiletheconductivegelachievestherequirementinmakingagoodcontactbetweentheelectrodesandskin,severalproblemsappear.Agel-free,non-contactelectrodebasedoncapacitivecouplingtheorywasprovidedinthispaper,whichwasintegratedontheprintcircuitboard(PCB).TheexperimentalresultsshowedthatclearECGsignalscouldbeacquiredinthelaboratoryconditionsbycouplingtheelectrodestothechestofpatientsthroughcottonbelts.

简介：Tworecentstudieshavediscoveredasurprisinglinkbetweenthreeseeminglydifferentclinicaldiseases:onethatdestroysbonemarrow,anotherthatpreventssundamagefrombeingrepaired,andathirdwhichcauseschildrentoshowsignsofagingextremelyearly.Ithasbeenfoundthatallthreediseasesarecausedbymutationsinthesamegene.Mutationscomeinanumberofdifferentflavors,

简介：ＰＲＥＬＩＭＩＮＡＲＹＲＥＳＥＡＲＣＨＯＦＨＵＭＡＮＨＥＰＡＴＯＣＹＴＥＣＥＬＬＬＩＮＥＡＳＴＨＥＢＩＯＬＯＧＩＣＡＬＭＡＴＥＲＩＡＬＯＦＥＬＡＤＰＲＥＬＩＭＩＮＡＲＹＲＥＳＥＡＲＣＨＯＦＨＵＭＡＮＨＥＰＡＴＯＣＹＴＥＣＥＬＬＬＩＮＥＡＳＴＨＥＢＩＯ...

简介：Theresearchgroupwasinterestedinthetherapeuticpotentialoflimbalfibroblasts,whicharepre-sentinthecorneallimbus.Recently,inmuchstemcellresearch,researchersandadvocatesarestress-ingtheimportanceofutilizingcellsfromthenichewhichtheywilltarget.Thismeans,thatadiposetissueandbonemarrowderivedstemcells,arelikelynotappropriateandevendangeroustoapplyu-biquitouslyastherapiestootherpartsofthebodyincertaincases.Severalresearchgroupsandclini-caltrialshaveshownthistobethecase,includingcasesofblindness,braininflammation,anddeathasadverseevents.