简介：Whitebackedplanthopper(WBPH)-resistanceinajaponica/indicadoubledhaploid(DH)ricepopulationestablishedfromacrossbetweenWBPH-resistantjaponicaChunjiang06andsusceptibleindicaTN1,wascomparativelyevaluatedthroughafieldexperimentbasedontheWBPHimmigrantdensityandstandardizedseedboxscreeningtest(SSST).AllthesusceptibleDHlinesinthefieldexperimentbehavedaccordinglyinSSST.However,35ofresistant66lines(53%)inthefield,werecategorizedtosusceptiblegroupsinSSST.Likewise,therewerenosignificantdifferencesinWBPHimmigrantdensitiesamong70DHlinesthatwerehighlyresistanttosusceptibleinSSST.TheresultsrevealedthatSSSTcouldnotevaluateproperlyWBPHresistanceintheDHlines.FourQTLsforWBPH-resistancephenotypedbytheimmigrantdensityweredetectedonchromosomes2,3,4,and11.Ofthem,theQTLonchromosome4wasthemosteffective(LOD21.8,variance78%).FiveQTLsassociatedwithseedlingmortalityweremappedonchromosomes2,3,4,5and6.InadditiontotheQTL(LOD10.5,variance68%)onchromosome4,therewasanothermajorQTL(LOD12.7,variance71%)locatedonchromosome5,whichwasSSST-specificbutmightbeirrespectiveoftheWBPHresistancetraits.

简介：Apopulationofricebrownplanthopper(BPH)NilaparvatalugenscollectedfromapaddyfieldinHangzhouwassuccessivelyrearedonsusceptiblericeTaichungNative1(TN1)inalaboratoryfreefrominsecticidesformorethan14generations.Thechangesinsusceptibilitytoinsecticidesandecologicalfitnessondifferentresistantricevarietiesweremonitoredineachgeneration.Theresistanceratiotoimidaclopridsharplydeclinedwiththesuccessionofrearinggenerationswithoutinsecticidesfrom359.94-foldatF1to6.50-foldatF14comparedwiththesusceptiblestrain,andtheresistanceratiotochlorpyrifoswasfrom9.90-foldatF1to5.94-foldatF14.Nymphaldurationandweightsofnewlyhatchedfemaleadultsweresignificantlyaffectedbyricevariety,generationandtheirinteractions,butnymphalsurvivalwassignificantlyaffectedbythegenerationonly.Theratioofbrachypterousadultsinmaleswasaffectedbythegenerationandgeneration×varietyinteraction,whereasnodifferencewasfoundinfemales.Nymphaldurationextendedwithincreasinggenerations,andthefemalenymphaldurationwasshorterinthesusceptiblevarietyTN1thanthoseintheresistantvarietiesIR26andIR36.Inaddition,thefemaleadultweightinTN1washigherthanthoseinIR26andIR36.TheseresultsindicatedthattheresistanceoffieldBPHpopulationtoinsecticideswasreversedafterseveralgenerationsofno-exposuretoinsecticides,andtheecologicalfitnessinTN1washigherthanthoseinIR26andIR36.ThesefindingssuggestedtherationalandreduceduseofinsecticidesincombinationwiththemanipulationofresistantricevarietieswouldbeeffectiveforBPHmanagement.