简介:AIMHumanglutathioneS-transferaseA1(GSTA1)isanimportantphaseⅡmetabolizingenzymeinvolvedinthemetabolismofmanytherapeuticdrugsandisresponsibleforthemetabolicdetoxificationofnumerouspromutagensandprocarcinogens.ThegeneticpolymorphismofGSTA1hasimportantimplicationsfordrugefficacyandcancersusceptibility.Inthisstudy,wedeterminedthedistributionofGSTA1geneticpolymorphisminMainlandChinese.Andwealsoinvestigatedwhetherthereexiststhepotentialphenotypealterationscausedbythegeneticpolymorphisminhuman.METHODSGenomicDNAwasex-tractedfromperipheralbloodof140Chinesepeopleand16livertissuesobtainedfromnon-liverishpatientswhounderwentpartialhepatectomy.AndthenthegenotypesofhumanGSTA1genewereanalyzedbypolymerasechainreaction-restrictedfragmentlengthpolymorphism(PCR-RFLP).