简介：Toelucidatethemechanismofphotodynamicdamageofcells,theeffectofHPDpluslightontranscriptlonalectlvltyInthemucleusIsolatefromthenormalratliverwasstudiedinvitorby3H-UTPincorporationintoRNA.MeasurementsoffluorescencespectrumshowedthatHPDwasboundtothenucleusanditsfluorescenceIntensityIncreasedwiththeIncreaseofHPDconcentration.TheexperimentalresultsIndicatedthatnochangescouldbeobservedwheneitherHPDorlightwasusedalone.WhereasthenucleartranscriptionactivitywasfoundtobeinhibitedsignificantlybybothHPDandlighttreatment,andthedegreeofInhibitionwasdependentontheHPDconcentrationandthetimeofexposuretolight.Aftertreatmentby3μg/mlHPD,theinhibitionrateofthenucleartranscriptionactivitywas23%,45%,69%,80%and90%,respectivelyforlightexposureof2,5,10,20and30minutes.Ourresultssuggestedthatdose-dependentdecreasesinthenucleartranscriptionactivity,andmarkedinhibitionofthe

简介：Duringautologousbonemarrowgraftintreatmentofmalignantdiseases,itiscriticaltopurgemalignantcellsfromthemarrow.Inthepresentstudy,thesensitivitytophotodynamicinactivationof3leukemiccelllineswascomparedwiththeircounterpartnormalhematopoieticcells.AftermouseleukemicL1210cellsweretreatedwithapreparationofhematoporphyrinderivatives,YHpD,10μg/mlfor1hr.andirradiatedwithblacklight(peakwavelength395nm,lightintensity0.6mW/cm2)for5minutes,thesurvivalrateofclonogeniccellsdecreasedto<10%,whilethatofbonemarrowgranulocytemacrophageprogenitorcells(CFU-GM)inDBA/2miceremainedatnearlynormallevel(>80%).SimilarresultswereobtainedwhenhumanleukemicHL-60cellswerecomparedwithhumanCFU-GMandmouseleukemicL615cellswithCFU-GMin615strainmice.ItissuggestedthathematoporphyrinphotoradiationmaybeusefulforIselectivelykillingleukemiccellsinbonemarrow.