简介：TostudythegeneticcharacterizationoffourstrainsofBorreliaburgdorferiisolatedinChina.PCRtechniquewasusedtoamplifythe5S-23SrRNAintergenicspacerDNAfromthewholecellularDNAofisolatedGXLD-4,9,18andChang14,andthentheamplifiedproductswereclonedintoplasmidpGEM-TEasyandsequenced.Itwasfoundthatthe5S-23SrRNAintergenicspacerDNAofthefourisolateswas242bp,revealingthenucleotidesequenceidentityofmorethan99%.ThefourisolateshadhighersequenceidentifywithBorreliavalaisianathanwithothergeneticgroups.ThesefourisolatesmostlikelybelongtoBorreliavalaisianagenomicgroup.
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