Induction Effect and Genetic Analysis of NG to Conchospores of Porphyra

(整期优先)网络出版时间:2002-02-12
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ThenewspreadingconchosporesotPorphyrayezoensis,P.haitanensisandP.katadaivar.hemiphyllaareinducedbyN-Methy-N'-Nitro-N-Nitrosoguanidine(NG),astrongmutagentoinducethemutantofchromatophore.Itisshowedthat(1)nomutantshavebeeninvestigatedinallcontrolgroup.(2)TheconchosporesofallthethreespeciesofPorphyraareeasytobeinducedbyNGfortheyareinthestageofbreeding,Inthelowestdose(10μg/mL)ofthisexperiment,themutantrateofP.yezoensisisupto57.9%,thatofP.haitanensis30.7%andthatofP.katadaivar.hemiphylla51.7%.(3)Themutantratecannotincreaseobviouslybyincreasingthereagentconcentrationorprolongingtheinductiontime.Withinthescopeofexperiment,theinducementeffectsofthreespeciesofPorphyraaremuchsimilar,andthemutantrateisnear50%.TheoptimuminductionconcentrationofNGis10μg/mL,andtheoptimuminductiontimeis30minutes.