简介：TofindoptimalconditionsforexpressingthesolubleformofsFv-2F3andtostudythepurificationandpropertyofitsderivativeSe-sFv-2F3,thepreferredexpressionconditionswereinvestigatedbymeansoforthogonaldesign.Thesecultureconditionsincludedincubationtemperature,inducerconcentration,inductiontimeandcellconcentration.TheevaluationofexpressionwasaccomplishedbytheanalysisofwholecelllysatesandtheyieldofsolublesFv-2F3wascalculatedaccordingtotheanalysisofProfinder(FTI-500,Pharmacia).Thepurificationprocedurewascarriedoutviaatwo-steppurificationprocedureconsistingofion-exchangechromatography,followedbyimmobilizedmetalaffinitychromatography(IMAC).TheantioxidantefficacyofSe-sFv-2F3wasdemonstratedbythedeterminationofthecontentofthemainproductoflipidperoxidation,MDA,theviabilityofcellsandtheactivityofLDH.WeobtainedthepreferredcultureconditionstogrowtheengineeredbacteriaandtheprocedureforpreparingsolublesFv-2F3andconfirmedtheantioxidantefficacyofSe-sFv-2F3.

简介：胶体的水晶模板或蛋白石与一包装结束以脸为中心立方(fcc)格子被准备从单音由垂直沉积驱散聚苯乙烯(PS)范围。为单体先锋的渗入的提供的空空格压克力填写了的模板从随后的copolymerization每硫酸盐，以及微胶化吃了酸，压克力酰胺和铵。样品为完全移开PS范围形成PAM反的蛋白石水疗院胶化(IOHPAM)或PAM/PAA反的蛋白石水疗院胶化(IOHPAM/PAA)沉浸于dimethylbenzenephotonic晶体。PS范围坐飞机被代替范围，它通过窗户互连对方。对为IOHPAM和IOHPAM/PAA电影有二座山峰的pH表演的回答的学习，而是IOHPAM/PAA达到顶点到更高的pH的移动，和山峰与AA内容是独立的。

简介：ToanalyzetheantitumorpotentialandmechanismofactionofsimultaneousNewcastlediseasevirus(NDV)hemagglutinin-neuraminidase(HN)andhumaninterleukin18(hIL-18)genetransferinC57BL/6micewithH22hepatoma,themousemodelwithH22hepatomawasestablishedinC57BL/6mice,andtheantitumoreffectsofthecombinedapplicationofNDVHNandhIL-18wereevaluatedinvivo.Theresultsshowthatthegrowthofestablishedtumorsinmiceimmunizedwithadenovirus(Ad)-HNinconjunctionwithAd-hIL-18wassignificantlyinhibitedcomparedwiththatinmiceimmunizedwithAd-HN,Ad-hIL-18alone,ortheemptyvector(Ad-mock).Furthermore,theimmunizationofmicewithAd-HNinconjunctionwithAd-hIL-18elicitedstrongnaturalkilleractivityandH22tumor-specificcytotoxicTlymphocyte(CTL)responsesinvivo.Inaddition,TcellsfromthelymphnodesofmiceimmunizedwithAd-hIL-18orAd-HN+Ad-hIL-18secretedhighlevelsoftheTh1cytokineIL-2andinterferon-γ(IFN-γ),indicatingthattheregressionoftumorcellsisrelatedtoaTh1-typedominantimmuneresponse.TheseresultsdemonstratethatvaccinationwithNDVHNtogetherwithhIL-18maybeanovelandpowerfulstrategyforcancerimmunotherapy.