简介:BACKGROUND:Nerveallograftrejectionisanunavoidableproblemfornerveallografts.Traumaticperipheralnerveinjuriesarecommonlyreconstructedusingautogenousnervegrafts.However,thisformofreconstructionislimitedbyinsufficientautologousnervesforlargegaprepairsandbymorbidityatthenervedonorsite.OBJECTIVE:ToexaminesciaticnerveregenerationandimmunetolerancereactionafterintragastricadministrationofultravioletB-irradiated(UVB)donorsplenocytes.DESIGN,TIMEANDSETTING:Acompleterandomizedgroupingdesignandcontrolledexperiment.TheexperimentswereconductedintheDepartmentofOrthopedics,theFirstAffiliatedHospitaltoShanxiMedicalUniversity,China,betweenMarchandOctober2007.MATERIALS:FourteenadultmaleSDratsandfourteenmaleWistarrats,weighing250–300g,wererandomlymatchedasdonorsandacceptors.AfurthersevenmaleSDrats(weight250–300g,age12–16weeks)wereusedfornerveisografts.ImmunepreparationsandtheEpicsXLflowcytometerwerepurchasedfromB-DCompany,USA.Acomputer-assistedelectromyographmachinewasprovidedbyKeypointP,DantelCompany,Denmark.METHODS:SplenocytesfromWistarratswereisolated,purified,andcultured,andthenirradiatedwithultravioletB.Inthefirstcontrolgroup(Group1),theSDratsreceivedasyngeneicSDnerveisograft.Inthesecondcontrolgroup(Group2),theSDratsreceivedanerveallograftfromWistarratswithoutpretreatment.Intheoral-tolerancetreatedgroup(Group3),theSDrecipientratswereinoculatedwith2.5×107LewisUVB-irradiateddonorsplenocytecellsbyintestinaltractadministrationatsevendaysbeforetransplantation.MAINOUTCOMEMEASURES:(1)Therecentendandthemiddleanddistalendofthetransplantednervewerecutat8and12weeksafteroperation.RecoveryofnerveregenerationwasmeasuredwithHEstainingusingthetotalnumber,density,anddiameterofthenervefibers.(2)ThelevelofCD25+Tlymphocytesinperipheralbloodwasdetec
简介:WithintheCNSnuclearfactor-kappaB(NF-κB)transcriptionfactorsareinvolvedinawiderangeoffunctionsbothinhomeostasisandinpathology.Overtheyears,ourandothergroupsproducedavastarrayofinformationonthecomplexinvolvementofNF-κBproteinsindifferentaspectsofpostnatalneurogenesisInparticular,severalextracellularsignalsandmembranereceptorshavebeenidentifiedasbeingabletoaffectneuralprogenitorcells(NPC)andtheirprogenyviaNF-κBactivation.AcrucialroleintheregulationofneuronalfatespecificationinadulthippocampalNPCisplayedbytheNF-κBp50subunit.NF-κBp50KOmicedisplayaremarkablereductioninadulthippocampalneurogenesiswhichcorrelateswithaselectivedefectinhippocampal-dependentshort-termmemory.MoreoverabsenceofNF-κBp50canprofoundlyaffecttheinvitroproneurogenicresponseofadulthippocampalNPC(ahNPC)toseveralendogenoussignalsanddrugs.HereinwebrieflyreviewthecurrentknowledgeonthepivotalroleofNF-κBp50intheregulationofadulthippocampalneurogenesis.InadditionwediscussmorerecentdatathatfurtherextendtherelevanceofNF-κBp50tonovelastroglia-derivedsignalswhichcaninfluenceneuronalspecificationofahNPCandtoastrocyte-NPCcross-talk.
简介:目的评价磁共振多体素波谱扫描技术对颞叶癫痫的定位诊断价值.方法选择具有明显颞叶癫痫表现,并经脑电图检查定位诊断明确的单侧颞叶癫痫患者30例作为研究对象;排除MRI检查存在颅脑肿瘤、脑血管畸形等症状性癫痫患者.患者均行常规MRI及双侧海马区多体素波谱扫描,完成检查后将以代谢物峰值含量为灰阶的伪彩图与倾斜冠状重度T2WI图像进行融合,测量双侧海马区N-乙酰天冬氨酸、胆碱峰值含量和胆碱/N-乙酰天冬氨酸比值,并直观分析双侧海马区两代谢物的分布情况.结果患侧N-乙酰天冬氨酸峰值含量为(13587.61±3913.99)MRunits,健侧为(17683.10±5610.61)MRunits,患侧低于健侧差异有显著性意义(t=-6.728,P<0.001);患侧胆碱/N-乙酰天冬氨酸比值为(1.26±0.11),健侧比值为(1.01±0.14),患侧高于健侧差异亦有显著性意义(t=7.883,P<0.001).将两代谢物含量及比值伪彩图与常规MRI图像进行融合,可直观发现双侧海马区N-乙酰天冬氨酸峰值含量及胆碱/N-乙酰天冬氨酸比值存在明显差异.结论磁共振多体素波谱技术不仅可以通过对某些代谢物含量的定量分析进行癫痫灶的定位、定侧诊断,还可通过常规MRI图像与多体素磁共振波谱图像的融合图像直观评价代谢物分布状态,描记癫痫灶的大致轮廓,为制定手术方案提供依据.
简介:目的研究大蒜素(allicin)在脊髓缺血再灌注损伤中的保护作用及其相关机制.方法实验前1w实验用SD大鼠预先给予不同浓度大蒜素腹腔注射,每天一次持续7d,采用肾下腹主动脉阻断法建立脊髓缺血再灌注损伤模型.损伤后24h通过测定脊髓组织含水量、梗死体积和正常神经元计数评价大鼠脊髓组织损伤程度.采用(Basso-Beattie-Bresnahan,BBB)评分标准对大鼠进行后肢功能评分以反映损伤后运动功能.损伤后4h和24h检测线粒体膜电位、活性氧自由基(ROS)含量和线粒体ATP的变化,讨论大蒜素保护作用与线粒体信号通路的关系.结果大蒜素能减轻脊髓缺血再灌注损伤,促进运动功能恢复,抑制线粒体功能障碍.结论大蒜素通过保护线粒体功能发挥对脊髓缺血再灌注损伤的保护作用.
简介:目的观察脑梗死合并胰岛素抵抗患者内脏脂肪堆积及其相关因素.方法收集病程在2h至3d的急性脑梗死患者93例,随机分为胰岛素抵抗组45例和非胰岛素抵抗组41例.采用彩色多普勒超声仪测量颈总动脉的内中膜厚度及其斑块以及内脏脂肪厚度.结果两组患者相比内脏脂肪厚度差异有统计学意义(P<0.01).内脏脂肪厚度分别与腰臀比(r=0.361,P=0.000)、空腹胰岛素(r=0.291,P=0.005)、胰岛素抵抗指数(r=0.326,P=0.001)、三酰甘油(r=0.319,P=0.002)、内中膜厚度(r=0.433,P=0.000)、空腹血糖(r=0.215,P=0.039)、2h血浆葡萄糖浓度(r=0.243,P=0.019)、腹壁脂肪厚度(r=0.220,P=0.034)呈正相关.结论脑梗死患者合并胰岛素抵抗时内脏脂肪堆积程度明显增高,且与动脉粥样硬化程度密切相关.提示,胰岛素抵抗时存在内脏脂肪堆积,并且可以加重动脉硬化的程度.
简介:目的利用RNA干扰(RNAinterference,RNAi)技术特定沉默胶质瘤U251细胞株的血小板源生长因子-B(PDGF-B)基因,观察其对U251细胞株细胞凋亡和增殖的影响。方法利用脂质体将针对PDGF-B基因的siRNA转染进入U251细胞,利用实时荧光定量多聚核苷酸链式反应(RTPCR)检测PDGF-B基因表达;Westernblot检测显示siRNA转染组PDGF-B蛋白表达,采用MTT法检测胶质瘤U251细胞的增殖,应用流式细胞计数观察抑制PDGF-B基因后胶质瘤U251细胞的凋亡情况。结果RT-PCR检测PDGF-B基因表达明显下降;Westernblot检测显示siRNA转染组PDGF-B蛋白表达明显抑制(抑制率〉60%),MTT结果显示siRNA转染组U251细胞增殖较对照组明显降低;流式细胞学检测提示降低PDGF-B在胶质瘤细胞的表达能抑制胶质瘤细胞的有丝分裂,促进细胞的凋亡。结论构建针对胶质瘤细胞PDGF-B的RNA干扰质粒并转染人胶质瘤U251细胞株后,可明显抑制U251细胞株PDGF的表达,对人胶质瘤U251细胞株有明显的生长抑制和促进凋亡作用。
简介:ThedefiningneuropathologicalfeatureofParkinson’sdisease(PD)isthelossofnigrostriataldopaminergic(DA)projections.Thisresultsinabiochemicalreductionofstriataldopaminelevelsandmovementdisorders,suchasatremoratrest,rigidityofthelimbs,bradykinesia,andposturalinstability(Kimetal.,2011;Kimetal.,2012;BurkeandO’Malley,2013;Leemetal.,2014;Nametal.,2014).BecausetheetiologyofPDisnotfullyunderstood,itcannotguidethedevelopmentofknowledge-basedtargetedtherapeutics.